Biofilm extracellular-DNA in 55 Staphylococcus epidermidis clinical isolates from implant infections

Stefano Ravaioli, Davide Campoccia, Livia Visai, Valter Pirini, Ilaria Cangini, Tolmino Corazzari, Alessandra Maso, Claudio Poggio, Francesco Pegreffi, Lucio Montanaro, Carla Renata Arciola

Research output: Contribution to journalArticlepeer-review


Biofilm formation is broadly recognized as an important virulence factor in many bacterial species implicated in implant-related opportunistic infections. In spite of a long history of research and many investigative efforts aimed at elucidating their chemical composition, structure, and function, the nature of bacterial biofilms still remains only partly revealed. Over the years, different extracellular polymeric substances (EPS) have been described that contribute functionally and structurally to the organization of biofilms. Recently extracellular DNA (eDNA) has emerged as a quantitatively conspicuous and potentially relevant structural component of microbial biofilms of many microbial species, Staphylococcus aureus and S. epidermidis among them. The present study aims at comparatively investigating the amount of eDNA present in the biofilm of 55 clinical isolates of S. epidermidis from postsurgical and biomaterial-related orthopedic infections. Quantification of eDNA was performed by a non-destructive method directly on bacterial biofilms formed under static conditions on the plastic surface of 96-well plates.

Original languageEnglish
Pages (from-to)840-846
Number of pages7
JournalInternational Journal of Artificial Organs
Issue number9
Publication statusPublished - Sep 2011


  • Biofilm
  • Exopopolysaccaride
  • Extracellular DNA
  • Implant orthopedic infections
  • Staphylococcus epidermidis

ASJC Scopus subject areas

  • Biomaterials
  • Biomedical Engineering
  • Bioengineering
  • Medicine (miscellaneous)


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