TY - JOUR
T1 - Biofilm formation and release of fluoride from dental restorative materials in relation to their surface properties
AU - Hahnel, Sebastian
AU - Ionescu, Andrei C.
AU - Cazzaniga, Gloria
AU - Ottobelli, Marco
AU - Brambilla, Eugenio
PY - 2017/5
Y1 - 2017/5
N2 - Objectives: To elucidate the impact of surface properties and the release of fluoride from different glass ionomer cements on biofilm formation. Methods: Standardized specimens manufactured from various classes of glass ionomer cements (GICs), a resin-based composite (RBC), and human enamel were subjected to surface analyses. Subsequent to simulation of salivary pellicle formation, Streptococcus mutans biofilm formation was initiated using a drip flow reactor for 48. h and 96. h. Biofilms were characterized by determining viable bacterial biomass and 3D biofilm architecture using SEM and CLSM; the release of fluoride from the specimens was measured using the ion selective micro method in dependence on various experimental conditions (incubation with sterile broth/bacteria/acid). Results: Surface properties and biofilm formation correlated poorly, while the release of fluoride correlated well with viable streptococcal biomass and SEM/CLSM analyses. For all investigated materials, biofilm formation was lower than on enamel. The release of fluoride showed a significant dependency on the experimental conditions applied; the presence of biofilms reduced fluoride release in comparison to sterile incubation conditions. Conclusions: Within the limitations of a laboratory study, the results suggest that biofilm formation on GICs cannot be easily predicted as a function of substratum surface parameters. The release of fluoride from glass ionomer cements contributes to control biofilm formation particularly in its early phases. Clinical significance: Glass ionomer cements can actively control microbial biofilm formation, while biofilms modulate the release of fluoride from GIC materials.
AB - Objectives: To elucidate the impact of surface properties and the release of fluoride from different glass ionomer cements on biofilm formation. Methods: Standardized specimens manufactured from various classes of glass ionomer cements (GICs), a resin-based composite (RBC), and human enamel were subjected to surface analyses. Subsequent to simulation of salivary pellicle formation, Streptococcus mutans biofilm formation was initiated using a drip flow reactor for 48. h and 96. h. Biofilms were characterized by determining viable bacterial biomass and 3D biofilm architecture using SEM and CLSM; the release of fluoride from the specimens was measured using the ion selective micro method in dependence on various experimental conditions (incubation with sterile broth/bacteria/acid). Results: Surface properties and biofilm formation correlated poorly, while the release of fluoride correlated well with viable streptococcal biomass and SEM/CLSM analyses. For all investigated materials, biofilm formation was lower than on enamel. The release of fluoride showed a significant dependency on the experimental conditions applied; the presence of biofilms reduced fluoride release in comparison to sterile incubation conditions. Conclusions: Within the limitations of a laboratory study, the results suggest that biofilm formation on GICs cannot be easily predicted as a function of substratum surface parameters. The release of fluoride from glass ionomer cements contributes to control biofilm formation particularly in its early phases. Clinical significance: Glass ionomer cements can actively control microbial biofilm formation, while biofilms modulate the release of fluoride from GIC materials.
KW - Biofilm
KW - Fluoride
KW - Glass ionomer cement
KW - Resin composite
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U2 - 10.1016/j.jdent.2017.02.005
DO - 10.1016/j.jdent.2017.02.005
M3 - Article
AN - SCOPUS:85015267141
VL - 60
SP - 14
EP - 24
JO - Journal of Dentistry
JF - Journal of Dentistry
SN - 0300-5712
ER -