Biofilm formation on titanium implants counteracted by grafting gallium and silver ions

Andrea Cochis, Barbara Azzimonti, Cinzia Della Valle, Roberto Chiesa, Carla Renata Arciola, Lia Rimondini

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Biofilm-associated infections remain the leading cause of implant failure. Thanks to its established biocompat-ibility and biomechanical properties, titanium has become one of the most widely used materials for bone implants. Engineered surface modifications of titanium able to thwart biofilm formation while endowing a safe anchorage to eukaryotic cells are being progressively developed. Here surfaces of disks of commercial grade 2 titanium for bone implant were grafted with gallium and silver ions by anodic spark deposition. Scanning electron microscopy of the surface morphology and energy dispersive X-ray spectroscopy were used for characterization. Gallium-grafted titanium was evaluated in comparison with silver-grafted titanium for both in vivo and in vitro antibiofilm properties and for in vitro compatibility with human primary gingival fibroblasts. Surface-modified materials showed: (i) homogeneous porous morphology, with pores of micrometric size; (ii) absence of cytotoxic effects; (iii) ability to support in vitro the adhesion and spreading of gingival fibroblasts; and (iv) antibiofilm properties. Although both silver and gallium exhibited in vitro strong antibacterial properties, in vivo gallium was significantly more effective than silver in reducing number and viability of biofilm bacteria colonies. Gallium-based treatments represent promising titanium antibiofilm coatings to develop new bone implantable devices for oral, maxillofacial, and orthopedic applications.

Original languageEnglish
Pages (from-to)1176-1187
Number of pages12
JournalJournal of Biomedical Materials Research - Part A
Volume103
Issue number3
DOIs
Publication statusPublished - Mar 1 2015

Fingerprint

Gallium
Biofilms
Titanium
Silver
Ions
Bone
Fibroblasts
Bone and Bones
X-Ray Emission Spectrometry
Microbial Viability
Orthopedics
Eukaryotic Cells
Electric sparks
Interfacial energy
Electron Scanning Microscopy
Surface morphology
Surface treatment
Bacteria
Adhesion
Equipment and Supplies

Keywords

  • Anodic spark deposition
  • Biofilm
  • Biomaterials
  • Implant infections

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials
  • Ceramics and Composites
  • Metals and Alloys
  • Medicine(all)

Cite this

Biofilm formation on titanium implants counteracted by grafting gallium and silver ions. / Cochis, Andrea; Azzimonti, Barbara; Della Valle, Cinzia; Chiesa, Roberto; Arciola, Carla Renata; Rimondini, Lia.

In: Journal of Biomedical Materials Research - Part A, Vol. 103, No. 3, 01.03.2015, p. 1176-1187.

Research output: Contribution to journalArticle

Cochis, Andrea ; Azzimonti, Barbara ; Della Valle, Cinzia ; Chiesa, Roberto ; Arciola, Carla Renata ; Rimondini, Lia. / Biofilm formation on titanium implants counteracted by grafting gallium and silver ions. In: Journal of Biomedical Materials Research - Part A. 2015 ; Vol. 103, No. 3. pp. 1176-1187.
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