Abstract
Biofilm-associated infections remain the leading cause of implant failure. Thanks to its established biocompat-ibility and biomechanical properties, titanium has become one of the most widely used materials for bone implants. Engineered surface modifications of titanium able to thwart biofilm formation while endowing a safe anchorage to eukaryotic cells are being progressively developed. Here surfaces of disks of commercial grade 2 titanium for bone implant were grafted with gallium and silver ions by anodic spark deposition. Scanning electron microscopy of the surface morphology and energy dispersive X-ray spectroscopy were used for characterization. Gallium-grafted titanium was evaluated in comparison with silver-grafted titanium for both in vivo and in vitro antibiofilm properties and for in vitro compatibility with human primary gingival fibroblasts. Surface-modified materials showed: (i) homogeneous porous morphology, with pores of micrometric size; (ii) absence of cytotoxic effects; (iii) ability to support in vitro the adhesion and spreading of gingival fibroblasts; and (iv) antibiofilm properties. Although both silver and gallium exhibited in vitro strong antibacterial properties, in vivo gallium was significantly more effective than silver in reducing number and viability of biofilm bacteria colonies. Gallium-based treatments represent promising titanium antibiofilm coatings to develop new bone implantable devices for oral, maxillofacial, and orthopedic applications.
| Original language | English |
|---|---|
| Pages (from-to) | 1176-1187 |
| Number of pages | 12 |
| Journal | Journal of Biomedical Materials Research - Part A |
| Volume | 103 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - Mar 1 2015 |
Fingerprint
Keywords
- Anodic spark deposition
- Biofilm
- Biomaterials
- Implant infections
ASJC Scopus subject areas
- Biomedical Engineering
- Biomaterials
- Ceramics and Composites
- Metals and Alloys
- Medicine(all)
Cite this
Biofilm formation on titanium implants counteracted by grafting gallium and silver ions. / Cochis, Andrea; Azzimonti, Barbara; Della Valle, Cinzia; Chiesa, Roberto; Arciola, Carla Renata; Rimondini, Lia.
In: Journal of Biomedical Materials Research - Part A, Vol. 103, No. 3, 01.03.2015, p. 1176-1187.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Biofilm formation on titanium implants counteracted by grafting gallium and silver ions
AU - Cochis, Andrea
AU - Azzimonti, Barbara
AU - Della Valle, Cinzia
AU - Chiesa, Roberto
AU - Arciola, Carla Renata
AU - Rimondini, Lia
PY - 2015/3/1
Y1 - 2015/3/1
N2 - Biofilm-associated infections remain the leading cause of implant failure. Thanks to its established biocompat-ibility and biomechanical properties, titanium has become one of the most widely used materials for bone implants. Engineered surface modifications of titanium able to thwart biofilm formation while endowing a safe anchorage to eukaryotic cells are being progressively developed. Here surfaces of disks of commercial grade 2 titanium for bone implant were grafted with gallium and silver ions by anodic spark deposition. Scanning electron microscopy of the surface morphology and energy dispersive X-ray spectroscopy were used for characterization. Gallium-grafted titanium was evaluated in comparison with silver-grafted titanium for both in vivo and in vitro antibiofilm properties and for in vitro compatibility with human primary gingival fibroblasts. Surface-modified materials showed: (i) homogeneous porous morphology, with pores of micrometric size; (ii) absence of cytotoxic effects; (iii) ability to support in vitro the adhesion and spreading of gingival fibroblasts; and (iv) antibiofilm properties. Although both silver and gallium exhibited in vitro strong antibacterial properties, in vivo gallium was significantly more effective than silver in reducing number and viability of biofilm bacteria colonies. Gallium-based treatments represent promising titanium antibiofilm coatings to develop new bone implantable devices for oral, maxillofacial, and orthopedic applications.
AB - Biofilm-associated infections remain the leading cause of implant failure. Thanks to its established biocompat-ibility and biomechanical properties, titanium has become one of the most widely used materials for bone implants. Engineered surface modifications of titanium able to thwart biofilm formation while endowing a safe anchorage to eukaryotic cells are being progressively developed. Here surfaces of disks of commercial grade 2 titanium for bone implant were grafted with gallium and silver ions by anodic spark deposition. Scanning electron microscopy of the surface morphology and energy dispersive X-ray spectroscopy were used for characterization. Gallium-grafted titanium was evaluated in comparison with silver-grafted titanium for both in vivo and in vitro antibiofilm properties and for in vitro compatibility with human primary gingival fibroblasts. Surface-modified materials showed: (i) homogeneous porous morphology, with pores of micrometric size; (ii) absence of cytotoxic effects; (iii) ability to support in vitro the adhesion and spreading of gingival fibroblasts; and (iv) antibiofilm properties. Although both silver and gallium exhibited in vitro strong antibacterial properties, in vivo gallium was significantly more effective than silver in reducing number and viability of biofilm bacteria colonies. Gallium-based treatments represent promising titanium antibiofilm coatings to develop new bone implantable devices for oral, maxillofacial, and orthopedic applications.
KW - Anodic spark deposition
KW - Biofilm
KW - Biomaterials
KW - Implant infections
UR - http://www.scopus.com/inward/record.url?scp=84923035774&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84923035774&partnerID=8YFLogxK
U2 - 10.1002/jbm.a.35270
DO - 10.1002/jbm.a.35270
M3 - Article
C2 - 25044610
AN - SCOPUS:84923035774
VL - 103
SP - 1176
EP - 1187
JO - Journal of Biomedical Materials Research - Part A
JF - Journal of Biomedical Materials Research - Part A
SN - 1549-3296
IS - 3
ER -