Biofunctional alendronate-Hydroxyapatite thin films deposited by Matrix Assisted Pulsed Laser Evaporation

Adriana Bigi, Elisa Boanini, Chiara Capuccini, Milena Fini, Ion N. Mihailescu, Carmen Ristoscu, Felix Sima, Paola Torricelli

Research output: Contribution to journalArticle

Abstract

We applied Matrix Assisted Pulsed Laser Evaporation (MAPLE) in order to synthesize alendronate-hydroxyapatite thin films on titanium substrates. Alendronate-hydroxyapatite composite nanocrystals with increasing bisphosphonate content (0, 3.9, 7.1% wt) were synthesized in aqueous medium. Then, they were suspended in deionised water, frozen at liquid nitrogen temperature and used as targets for MAPLE experiments. The depositions were conducted with a KrF* excimer laser source (l = 248 nm, tFWHM = 25 ns) in mild conditions of temperature and pressure. The obtained thin films had a good crystallinity, which slightly decreases with the increase of alendronate content, and exhibited a porous-like structure. Osteoblast-like MG63 cells and human osteoclasts were cultured on the thin films up to 14 days. In the presence of alendronate, MG63 cells displayed a normal morphology, increased proliferation and higher values of differentiation parameters, namely type I collagen, osteocalcin, and osteoprotegerin/TNF-related activation-induced cytokine receptor ratio. In contrast, osteoclasts showed significantly reduced proliferation, and increased level of Caspase 3. Moreover, the coatings synthesized from hydroxyapatite at relatively high bisphosphonate content (7.1% wt) displayed a reduced production of Tumour Necrosis Factor alpha (TNF-α) and Interleukin 6 (IL-6), suggesting a down-regulatory role of alendronate on the inflammatory reaction. The successful deposition of alendronate modified hydroxyapatite thin films yields coatings with enhanced bioactivity, able to promote osteoblast differentiation and to inhibit osteoclast proliferation.

Original languageEnglish
Pages (from-to)6168-6177
Number of pages10
JournalBiomaterials
Volume30
Issue number31
DOIs
Publication statusPublished - Oct 2009

Fingerprint

Alendronate
Durapatite
Pulsed lasers
Hydroxyapatite
Evaporation
Lasers
Thin films
Osteoblasts
Osteoclasts
Diphosphonates
Coatings
Deionized water
Excimer lasers
Liquid nitrogen
Tumor Necrosis Factor-alpha
Bioactivity
Collagen
Nanocrystals
RANK Ligand
Osteoprotegerin

Keywords

  • Alendronate-doped hydroxyapatite films
  • Bisphosphonate
  • MAPLE
  • Osteoblast
  • Osteoclast

ASJC Scopus subject areas

  • Biomaterials
  • Bioengineering
  • Ceramics and Composites
  • Mechanics of Materials
  • Biophysics

Cite this

Biofunctional alendronate-Hydroxyapatite thin films deposited by Matrix Assisted Pulsed Laser Evaporation. / Bigi, Adriana; Boanini, Elisa; Capuccini, Chiara; Fini, Milena; Mihailescu, Ion N.; Ristoscu, Carmen; Sima, Felix; Torricelli, Paola.

In: Biomaterials, Vol. 30, No. 31, 10.2009, p. 6168-6177.

Research output: Contribution to journalArticle

Bigi, Adriana ; Boanini, Elisa ; Capuccini, Chiara ; Fini, Milena ; Mihailescu, Ion N. ; Ristoscu, Carmen ; Sima, Felix ; Torricelli, Paola. / Biofunctional alendronate-Hydroxyapatite thin films deposited by Matrix Assisted Pulsed Laser Evaporation. In: Biomaterials. 2009 ; Vol. 30, No. 31. pp. 6168-6177.
@article{a423b026e7934302a2bfadb3a317f343,
title = "Biofunctional alendronate-Hydroxyapatite thin films deposited by Matrix Assisted Pulsed Laser Evaporation",
abstract = "We applied Matrix Assisted Pulsed Laser Evaporation (MAPLE) in order to synthesize alendronate-hydroxyapatite thin films on titanium substrates. Alendronate-hydroxyapatite composite nanocrystals with increasing bisphosphonate content (0, 3.9, 7.1{\%} wt) were synthesized in aqueous medium. Then, they were suspended in deionised water, frozen at liquid nitrogen temperature and used as targets for MAPLE experiments. The depositions were conducted with a KrF* excimer laser source (l = 248 nm, tFWHM = 25 ns) in mild conditions of temperature and pressure. The obtained thin films had a good crystallinity, which slightly decreases with the increase of alendronate content, and exhibited a porous-like structure. Osteoblast-like MG63 cells and human osteoclasts were cultured on the thin films up to 14 days. In the presence of alendronate, MG63 cells displayed a normal morphology, increased proliferation and higher values of differentiation parameters, namely type I collagen, osteocalcin, and osteoprotegerin/TNF-related activation-induced cytokine receptor ratio. In contrast, osteoclasts showed significantly reduced proliferation, and increased level of Caspase 3. Moreover, the coatings synthesized from hydroxyapatite at relatively high bisphosphonate content (7.1{\%} wt) displayed a reduced production of Tumour Necrosis Factor alpha (TNF-α) and Interleukin 6 (IL-6), suggesting a down-regulatory role of alendronate on the inflammatory reaction. The successful deposition of alendronate modified hydroxyapatite thin films yields coatings with enhanced bioactivity, able to promote osteoblast differentiation and to inhibit osteoclast proliferation.",
keywords = "Alendronate-doped hydroxyapatite films, Bisphosphonate, MAPLE, Osteoblast, Osteoclast",
author = "Adriana Bigi and Elisa Boanini and Chiara Capuccini and Milena Fini and Mihailescu, {Ion N.} and Carmen Ristoscu and Felix Sima and Paola Torricelli",
year = "2009",
month = "10",
doi = "10.1016/j.biomaterials.2009.07.066",
language = "English",
volume = "30",
pages = "6168--6177",
journal = "Biomaterials",
issn = "0142-9612",
publisher = "Elsevier BV",
number = "31",

}

TY - JOUR

T1 - Biofunctional alendronate-Hydroxyapatite thin films deposited by Matrix Assisted Pulsed Laser Evaporation

AU - Bigi, Adriana

AU - Boanini, Elisa

AU - Capuccini, Chiara

AU - Fini, Milena

AU - Mihailescu, Ion N.

AU - Ristoscu, Carmen

AU - Sima, Felix

AU - Torricelli, Paola

PY - 2009/10

Y1 - 2009/10

N2 - We applied Matrix Assisted Pulsed Laser Evaporation (MAPLE) in order to synthesize alendronate-hydroxyapatite thin films on titanium substrates. Alendronate-hydroxyapatite composite nanocrystals with increasing bisphosphonate content (0, 3.9, 7.1% wt) were synthesized in aqueous medium. Then, they were suspended in deionised water, frozen at liquid nitrogen temperature and used as targets for MAPLE experiments. The depositions were conducted with a KrF* excimer laser source (l = 248 nm, tFWHM = 25 ns) in mild conditions of temperature and pressure. The obtained thin films had a good crystallinity, which slightly decreases with the increase of alendronate content, and exhibited a porous-like structure. Osteoblast-like MG63 cells and human osteoclasts were cultured on the thin films up to 14 days. In the presence of alendronate, MG63 cells displayed a normal morphology, increased proliferation and higher values of differentiation parameters, namely type I collagen, osteocalcin, and osteoprotegerin/TNF-related activation-induced cytokine receptor ratio. In contrast, osteoclasts showed significantly reduced proliferation, and increased level of Caspase 3. Moreover, the coatings synthesized from hydroxyapatite at relatively high bisphosphonate content (7.1% wt) displayed a reduced production of Tumour Necrosis Factor alpha (TNF-α) and Interleukin 6 (IL-6), suggesting a down-regulatory role of alendronate on the inflammatory reaction. The successful deposition of alendronate modified hydroxyapatite thin films yields coatings with enhanced bioactivity, able to promote osteoblast differentiation and to inhibit osteoclast proliferation.

AB - We applied Matrix Assisted Pulsed Laser Evaporation (MAPLE) in order to synthesize alendronate-hydroxyapatite thin films on titanium substrates. Alendronate-hydroxyapatite composite nanocrystals with increasing bisphosphonate content (0, 3.9, 7.1% wt) were synthesized in aqueous medium. Then, they were suspended in deionised water, frozen at liquid nitrogen temperature and used as targets for MAPLE experiments. The depositions were conducted with a KrF* excimer laser source (l = 248 nm, tFWHM = 25 ns) in mild conditions of temperature and pressure. The obtained thin films had a good crystallinity, which slightly decreases with the increase of alendronate content, and exhibited a porous-like structure. Osteoblast-like MG63 cells and human osteoclasts were cultured on the thin films up to 14 days. In the presence of alendronate, MG63 cells displayed a normal morphology, increased proliferation and higher values of differentiation parameters, namely type I collagen, osteocalcin, and osteoprotegerin/TNF-related activation-induced cytokine receptor ratio. In contrast, osteoclasts showed significantly reduced proliferation, and increased level of Caspase 3. Moreover, the coatings synthesized from hydroxyapatite at relatively high bisphosphonate content (7.1% wt) displayed a reduced production of Tumour Necrosis Factor alpha (TNF-α) and Interleukin 6 (IL-6), suggesting a down-regulatory role of alendronate on the inflammatory reaction. The successful deposition of alendronate modified hydroxyapatite thin films yields coatings with enhanced bioactivity, able to promote osteoblast differentiation and to inhibit osteoclast proliferation.

KW - Alendronate-doped hydroxyapatite films

KW - Bisphosphonate

KW - MAPLE

KW - Osteoblast

KW - Osteoclast

UR - http://www.scopus.com/inward/record.url?scp=69649088493&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=69649088493&partnerID=8YFLogxK

U2 - 10.1016/j.biomaterials.2009.07.066

DO - 10.1016/j.biomaterials.2009.07.066

M3 - Article

C2 - 19692118

AN - SCOPUS:69649088493

VL - 30

SP - 6168

EP - 6177

JO - Biomaterials

JF - Biomaterials

SN - 0142-9612

IS - 31

ER -