Bioink Composition and Printing Parameters for 3D Modeling Neural Tissue

Valentina Fantini, Matteo Bordoni, Franca Scocozza, Michele Conti, Eveljn Scarian, Stephana Carelli, Anna Maria Di Giulio, Stefania Marconi, Orietta Pansarasa, Ferdinando Auricchio, Cristina Cereda

Research output: Contribution to journalArticle

Abstract

Neurodegenerative diseases (NDs) are a broad class of pathologies characterized by the progressive loss of neurons in the central nervous system. The main problem in the study of NDs is the lack of an adequate realistic experimental model to study the pathogenic mechanisms. Induced pluripotent stem cells (iPSCs) partially overcome the problem, with their capability to differentiate into almost every cell types; even so, these cells alone are not sufficient to unveil the mechanisms underlying NDs. 3D bioprinting allows to control the distribution of cells such as neurons, leading to the creation of a realistic in vitro model. In this work, we analyzed two biomaterials: sodium alginate and gelatin, and three different cell types: a neuroblastoma cell line (SH-SY5Y), iPSCs, and neural stem cells. All cells were encapsulated inside the bioink, printed and cultivated for at least seven days; they all presented good viability. We also evaluated the maintenance of the printed shape, opening the possibility to obtain a reliable in vitro neural tissue combining 3D bioprinting and iPSCs technology, optimizing the study of the degenerative processes that are still widely unknown.

Original languageEnglish
JournalCells
Volume8
Issue number8
DOIs
Publication statusPublished - Aug 5 2019

Fingerprint

Neurodegenerative diseases
Stem cells
Printing
Induced Pluripotent Stem Cells
Bioprinting
Tissue
Neurodegenerative Diseases
Chemical analysis
Neurons
Neurology
Biocompatible Materials
Pathology
Gelatin
Neural Stem Cells
Neuroblastoma
Cells
Theoretical Models
Central Nervous System
Maintenance
Three Dimensional Printing

Cite this

Bioink Composition and Printing Parameters for 3D Modeling Neural Tissue. / Fantini, Valentina; Bordoni, Matteo; Scocozza, Franca; Conti, Michele; Scarian, Eveljn; Carelli, Stephana; Di Giulio, Anna Maria; Marconi, Stefania; Pansarasa, Orietta; Auricchio, Ferdinando; Cereda, Cristina.

In: Cells, Vol. 8, No. 8, 05.08.2019.

Research output: Contribution to journalArticle

Fantini, V, Bordoni, M, Scocozza, F, Conti, M, Scarian, E, Carelli, S, Di Giulio, AM, Marconi, S, Pansarasa, O, Auricchio, F & Cereda, C 2019, 'Bioink Composition and Printing Parameters for 3D Modeling Neural Tissue', Cells, vol. 8, no. 8. https://doi.org/10.3390/cells8080830
Fantini, Valentina ; Bordoni, Matteo ; Scocozza, Franca ; Conti, Michele ; Scarian, Eveljn ; Carelli, Stephana ; Di Giulio, Anna Maria ; Marconi, Stefania ; Pansarasa, Orietta ; Auricchio, Ferdinando ; Cereda, Cristina. / Bioink Composition and Printing Parameters for 3D Modeling Neural Tissue. In: Cells. 2019 ; Vol. 8, No. 8.
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