Bioinspired negatively charged calcium phosphate nanocarriers for cardiac delivery of MicroRNAs

Vittoria Di Mauro, Michele Iafisco, Nicolò Salvarani, Marco Vacchiano, Pierluigi Carullo, Gloria Belén Ramírez-Rodríguez, Tatiana Patrício, Anna Tampieri, Michele Miragoli, Daniele Catalucci

Research output: Contribution to journalArticle

Abstract

Aim: To develop biocompatible and bioresorbable negatively charged calcium phosphate nanoparticles (CaP-NPs) as an innovative therapeutic system for the delivery of bioactive molecules to the heart. Materials & methods: CaP-NPs were synthesized via a straightforward one-pot biomineralization-inspired protocol employing citrate as a stabilizing agent and regulator of crystal growth. CaP-NPs were administered to cardiac cells in vitro and effects of treatments were assessed. CaP-NPs were administered in vivo and delivery of microRNAs was evaluated. Results: CaP-NPs efficiently internalized into cardiomyocytes without promoting toxicity or interfering with any functional properties. CaP-NPs successfully encapsulated synthetic microRNAs, which were efficiently delivered into cardiac cells in vitro and in vivo. Conclusion: CaP-NPs are a safe and efficient drug-delivery system for potential therapeutic treatments of polarized cells such as cardiomyocytes.

Original languageEnglish
Pages (from-to)891-906
Number of pages16
JournalNanomedicine
Volume11
Issue number8
DOIs
Publication statusPublished - Apr 1 2016

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Keywords

  • calcium phosphate nanoparticles
  • cardiomyocytes
  • drug-delivery systems
  • microRNA
  • nanomedicine

ASJC Scopus subject areas

  • Materials Science(all)
  • Bioengineering
  • Biomedical Engineering
  • Medicine (miscellaneous)
  • Development

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