Biologic characterization of pleural metastases from lung adenocarcinoma: Description of the new DV90 cell line

F. Monti, S. Szymczuk, L. Chessa, S. Prudente, G. Nicoletti, P. P. Fattori, E. Pini, F. Desiderio, E. Pasquini, V. Tison, A. Ravaioli

Research output: Contribution to journalArticlepeer-review

Abstract

Aims and background: The characterization of pleural metastases from lung adenocarcinoma is often limited to single biologic features. Methods: The present paper describes the cellular kinetic parameters, as well as immunocytochemical, ultrastructural and genetic characteristics of the new DV90 cell line, established from the pleural effusion of a stage IV lung adenocarcinoma. Results: The cell line has a diploid DNA content, a doubling time of 24 h and 7% cloning efficiency, it is tumorigenic in nude mice. Ultrastructural investigation revealed the typical features of lung adenocarcinoma; the diagnosis was confirmed by its immunohistochemical reactivity with a panel of monoclonal antibodies specifically capable of identifying adenocarcinoma cells. Genetic analysis revealed a 46 X, -Y, +8, der (6)t(6?)(q27;?) karyotype and hyperexpression of the protein codified by genes Her2/Neu and p53. Conclusion: The importance of multidisciplinary biologic characterization in identifying the origin and biological behavior of pleural metastases deriving from lung adenocarcinoma is discussed.

Original languageEnglish
Pages (from-to)378-384
Number of pages7
JournalTumori
Volume80
Issue number5
Publication statusPublished - 1994

Keywords

  • cell line
  • lung adenocarcinoma
  • pleural metastasis

ASJC Scopus subject areas

  • Cancer Research

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