Biologic characterization of pleural metastases from lung adenocarcinoma: Description of the new DV90 cell line

F. Monti, S. Szymczuk, L. Chessa, S. Prudente, G. Nicoletti, P. P. Fattori, E. Pini, F. Desiderio, E. Pasquini, V. Tison, A. Ravaioli

Research output: Contribution to journalArticle

Abstract

Aims and background: The characterization of pleural metastases from lung adenocarcinoma is often limited to single biologic features. Methods: The present paper describes the cellular kinetic parameters, as well as immunocytochemical, ultrastructural and genetic characteristics of the new DV90 cell line, established from the pleural effusion of a stage IV lung adenocarcinoma. Results: The cell line has a diploid DNA content, a doubling time of 24 h and 7% cloning efficiency, it is tumorigenic in nude mice. Ultrastructural investigation revealed the typical features of lung adenocarcinoma; the diagnosis was confirmed by its immunohistochemical reactivity with a panel of monoclonal antibodies specifically capable of identifying adenocarcinoma cells. Genetic analysis revealed a 46 X, -Y, +8, der (6)t(6?)(q27;?) karyotype and hyperexpression of the protein codified by genes Her2/Neu and p53. Conclusion: The importance of multidisciplinary biologic characterization in identifying the origin and biological behavior of pleural metastases deriving from lung adenocarcinoma is discussed.

Original languageEnglish
Pages (from-to)378-384
Number of pages7
JournalTumori
Volume80
Issue number5
Publication statusPublished - 1994

Fingerprint

Neoplasm Metastasis
Cell Line
erbB-2 Genes
p53 Genes
Pleural Effusion
Diploidy
Karyotype
Nude Mice
Organism Cloning
Adenocarcinoma
Monoclonal Antibodies
Adenocarcinoma of lung
DNA
Proteins

Keywords

  • cell line
  • lung adenocarcinoma
  • pleural metastasis

ASJC Scopus subject areas

  • Cancer Research

Cite this

Monti, F., Szymczuk, S., Chessa, L., Prudente, S., Nicoletti, G., Fattori, P. P., ... Ravaioli, A. (1994). Biologic characterization of pleural metastases from lung adenocarcinoma: Description of the new DV90 cell line. Tumori, 80(5), 378-384.

Biologic characterization of pleural metastases from lung adenocarcinoma : Description of the new DV90 cell line. / Monti, F.; Szymczuk, S.; Chessa, L.; Prudente, S.; Nicoletti, G.; Fattori, P. P.; Pini, E.; Desiderio, F.; Pasquini, E.; Tison, V.; Ravaioli, A.

In: Tumori, Vol. 80, No. 5, 1994, p. 378-384.

Research output: Contribution to journalArticle

Monti, F, Szymczuk, S, Chessa, L, Prudente, S, Nicoletti, G, Fattori, PP, Pini, E, Desiderio, F, Pasquini, E, Tison, V & Ravaioli, A 1994, 'Biologic characterization of pleural metastases from lung adenocarcinoma: Description of the new DV90 cell line', Tumori, vol. 80, no. 5, pp. 378-384.
Monti, F. ; Szymczuk, S. ; Chessa, L. ; Prudente, S. ; Nicoletti, G. ; Fattori, P. P. ; Pini, E. ; Desiderio, F. ; Pasquini, E. ; Tison, V. ; Ravaioli, A. / Biologic characterization of pleural metastases from lung adenocarcinoma : Description of the new DV90 cell line. In: Tumori. 1994 ; Vol. 80, No. 5. pp. 378-384.
@article{074648b0671e42c9816e730a1a5e810e,
title = "Biologic characterization of pleural metastases from lung adenocarcinoma: Description of the new DV90 cell line",
abstract = "Aims and background: The characterization of pleural metastases from lung adenocarcinoma is often limited to single biologic features. Methods: The present paper describes the cellular kinetic parameters, as well as immunocytochemical, ultrastructural and genetic characteristics of the new DV90 cell line, established from the pleural effusion of a stage IV lung adenocarcinoma. Results: The cell line has a diploid DNA content, a doubling time of 24 h and 7{\%} cloning efficiency, it is tumorigenic in nude mice. Ultrastructural investigation revealed the typical features of lung adenocarcinoma; the diagnosis was confirmed by its immunohistochemical reactivity with a panel of monoclonal antibodies specifically capable of identifying adenocarcinoma cells. Genetic analysis revealed a 46 X, -Y, +8, der (6)t(6?)(q27;?) karyotype and hyperexpression of the protein codified by genes Her2/Neu and p53. Conclusion: The importance of multidisciplinary biologic characterization in identifying the origin and biological behavior of pleural metastases deriving from lung adenocarcinoma is discussed.",
keywords = "cell line, lung adenocarcinoma, pleural metastasis",
author = "F. Monti and S. Szymczuk and L. Chessa and S. Prudente and G. Nicoletti and Fattori, {P. P.} and E. Pini and F. Desiderio and E. Pasquini and V. Tison and A. Ravaioli",
year = "1994",
language = "English",
volume = "80",
pages = "378--384",
journal = "Tumori",
issn = "0300-8916",
publisher = "SAGE Publications Ltd",
number = "5",

}

TY - JOUR

T1 - Biologic characterization of pleural metastases from lung adenocarcinoma

T2 - Description of the new DV90 cell line

AU - Monti, F.

AU - Szymczuk, S.

AU - Chessa, L.

AU - Prudente, S.

AU - Nicoletti, G.

AU - Fattori, P. P.

AU - Pini, E.

AU - Desiderio, F.

AU - Pasquini, E.

AU - Tison, V.

AU - Ravaioli, A.

PY - 1994

Y1 - 1994

N2 - Aims and background: The characterization of pleural metastases from lung adenocarcinoma is often limited to single biologic features. Methods: The present paper describes the cellular kinetic parameters, as well as immunocytochemical, ultrastructural and genetic characteristics of the new DV90 cell line, established from the pleural effusion of a stage IV lung adenocarcinoma. Results: The cell line has a diploid DNA content, a doubling time of 24 h and 7% cloning efficiency, it is tumorigenic in nude mice. Ultrastructural investigation revealed the typical features of lung adenocarcinoma; the diagnosis was confirmed by its immunohistochemical reactivity with a panel of monoclonal antibodies specifically capable of identifying adenocarcinoma cells. Genetic analysis revealed a 46 X, -Y, +8, der (6)t(6?)(q27;?) karyotype and hyperexpression of the protein codified by genes Her2/Neu and p53. Conclusion: The importance of multidisciplinary biologic characterization in identifying the origin and biological behavior of pleural metastases deriving from lung adenocarcinoma is discussed.

AB - Aims and background: The characterization of pleural metastases from lung adenocarcinoma is often limited to single biologic features. Methods: The present paper describes the cellular kinetic parameters, as well as immunocytochemical, ultrastructural and genetic characteristics of the new DV90 cell line, established from the pleural effusion of a stage IV lung adenocarcinoma. Results: The cell line has a diploid DNA content, a doubling time of 24 h and 7% cloning efficiency, it is tumorigenic in nude mice. Ultrastructural investigation revealed the typical features of lung adenocarcinoma; the diagnosis was confirmed by its immunohistochemical reactivity with a panel of monoclonal antibodies specifically capable of identifying adenocarcinoma cells. Genetic analysis revealed a 46 X, -Y, +8, der (6)t(6?)(q27;?) karyotype and hyperexpression of the protein codified by genes Her2/Neu and p53. Conclusion: The importance of multidisciplinary biologic characterization in identifying the origin and biological behavior of pleural metastases deriving from lung adenocarcinoma is discussed.

KW - cell line

KW - lung adenocarcinoma

KW - pleural metastasis

UR - http://www.scopus.com/inward/record.url?scp=0028586002&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028586002&partnerID=8YFLogxK

M3 - Article

C2 - 7839470

AN - SCOPUS:0028586002

VL - 80

SP - 378

EP - 384

JO - Tumori

JF - Tumori

SN - 0300-8916

IS - 5

ER -