Biological activity of sea bass (Dicentrarchus labrax L.) recombinant interleukin-1β

Francesco Buonocore, Maria Forlenza, Elisa Randelli, Stefania Benedetti, Paola Bossù, Sabrina Meloni, Christopher J. Secombes, Massimo Mazzini, Giuseppe Scapigliati

Research output: Contribution to journalArticle

Abstract

Biological activities of a putative mature sea bass interleukin-1β peptide, produced as a recombinant protein (rIL-1β) in Escherichia coli, have been investigated. The rIL-1β contains a 6-histidine tag at the N-terminus, and protein purification has been achieved through this tag by affinity chromatography. Biological activities have been investigated both at the cellular and gene expression levels. In in vitro assays sea bass rIL-1β induced the proliferation of murine D10.G4.1 cells and increased yeast phagocytosis by sea bass head kidney leukocytes. The purified cytokine was also tested in a lymphocyte-activation factor assay, where it induced the proliferation of sea bass thymocytes. Finally, in an in vivo assay, rIL-1β administered intraperitoneally increased expression levels of the IL-1β gene and activated macrophages to produce a cyclooxygenase 2 homologue (COX-2) gene in the head kidney.

Original languageEnglish
Pages (from-to)609-617
Number of pages9
JournalMarine Biotechnology
Volume7
Issue number6
DOIs
Publication statusPublished - Dec 2005

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Keywords

  • Cyclooxygenase 2
  • Cytokine
  • European sea bass
  • Interleukin 1β
  • Phagocytosis
  • Proliferation

ASJC Scopus subject areas

  • Aquatic Science
  • Biotechnology
  • Biochemistry

Cite this

Buonocore, F., Forlenza, M., Randelli, E., Benedetti, S., Bossù, P., Meloni, S., Secombes, C. J., Mazzini, M., & Scapigliati, G. (2005). Biological activity of sea bass (Dicentrarchus labrax L.) recombinant interleukin-1β. Marine Biotechnology, 7(6), 609-617. https://doi.org/10.1007/s10126-004-5131-5