Biological dynamics of hepatitis B virus load in dialysis population

Fabrizio Fabrizi, Giovanna Lunghi, Giancarlo Alongi, Sergio Bisegna, Gesualdo Campolo, Stefano Mangano, Aurelio Limido, Bruno Pagliari, Fabio Tettamanzi, Claudio Ponticelli

Research output: Contribution to journalArticlepeer-review

Abstract

Background: Control of the spread of hepatitis B virus (HBV) infection in dialysis units has been one of major advances in the management of end-stage renal disease. However, the natural history of HBV in dialysis patients remains unclear. The aim of this study is to measure monthly HBV viral load (HBV DNA) in a large cohort (n = 29) of hepatitis B surface antigen (HBsAg)-positive chronic dialysis patients during 12 months. Methods: HBV DNA was measured using the Amplicor HBV Monitor Test (Roche Diagnostics, Branchburg, NJ), an in vitro assay using polymerase chain reaction nucleic acid amplification and DNA hybridization for the quantitative measurement of HBV DNA in serum. Results: We observed three HBV DNA patterns: (1) patients persistently positive by Amplicor HBV Monitor Test (persistent HBV DNA; 7 of 29 patients; 24.1%), (2) individuals with alternatively positive and negative results (intermittent HBV DNA; 18 of 29 patients; 62.1%), and (3) patients persistently negative by Amplicor HBV Monitor Test (4 of 29 patients; 13.8%). HBV viral load was greater in patients with persistent compared with intermittent HBV DNA (persistently HBV DNA positive; 2.686 × 104 copies/mL; 95% confidence interval [CI], 5.2499 × 104 to 1.8158 × 104 copies/mL) versus intermittently HBV DNA positive (1.071 × 103 copies/mL; 95% CI, 8.524 × 103 to 4.09 × 102 copies/mL; P = 0.0001). In the entire group, HBV load at study entry was low and did not change versus the end of follow-up. Conclusion: Three patterns of HBV viremia in dialysis patients over time were assessed; HBV load was not high and was relatively stable. HBsAg-positive patients who were intermittently HBV DNA positive had less HBV viral load than persistently HBV DNA-positive patients. Periodic testing for HBV DNA to assess the virological status of HBsAg-positive dialysis patients is recommended.

Original languageEnglish
Pages (from-to)1278-1285
Number of pages8
JournalAmerican Journal of Kidney Diseases
Volume41
Issue number6
DOIs
Publication statusPublished - Jun 1 2003

Keywords

  • Chronic hepatitis B virus (HBV) infection
  • Dialysis
  • Fluctuations
  • Hepatitis B virus viremia (HBV DNA)

ASJC Scopus subject areas

  • Nephrology

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