Biological dynamics of hepatitis B virus load in dialysis population

Fabrizio Fabrizi, Giovanna Lunghi, Giancarlo Alongi, Sergio Bisegna, Gesualdo Campolo, Stefano Mangano, Aurelio Limido, Bruno Pagliari, Fabio Tettamanzi, Claudio Ponticelli

Research output: Contribution to journalArticle

Abstract

Background: Control of the spread of hepatitis B virus (HBV) infection in dialysis units has been one of major advances in the management of end-stage renal disease. However, the natural history of HBV in dialysis patients remains unclear. The aim of this study is to measure monthly HBV viral load (HBV DNA) in a large cohort (n = 29) of hepatitis B surface antigen (HBsAg)-positive chronic dialysis patients during 12 months. Methods: HBV DNA was measured using the Amplicor HBV Monitor Test (Roche Diagnostics, Branchburg, NJ), an in vitro assay using polymerase chain reaction nucleic acid amplification and DNA hybridization for the quantitative measurement of HBV DNA in serum. Results: We observed three HBV DNA patterns: (1) patients persistently positive by Amplicor HBV Monitor Test (persistent HBV DNA; 7 of 29 patients; 24.1%), (2) individuals with alternatively positive and negative results (intermittent HBV DNA; 18 of 29 patients; 62.1%), and (3) patients persistently negative by Amplicor HBV Monitor Test (4 of 29 patients; 13.8%). HBV viral load was greater in patients with persistent compared with intermittent HBV DNA (persistently HBV DNA positive; 2.686 × 104 copies/mL; 95% confidence interval [CI], 5.2499 × 104 to 1.8158 × 104 copies/mL) versus intermittently HBV DNA positive (1.071 × 103 copies/mL; 95% CI, 8.524 × 103 to 4.09 × 102 copies/mL; P = 0.0001). In the entire group, HBV load at study entry was low and did not change versus the end of follow-up. Conclusion: Three patterns of HBV viremia in dialysis patients over time were assessed; HBV load was not high and was relatively stable. HBsAg-positive patients who were intermittently HBV DNA positive had less HBV viral load than persistently HBV DNA-positive patients. Periodic testing for HBV DNA to assess the virological status of HBsAg-positive dialysis patients is recommended.

Original languageEnglish
Pages (from-to)1278-1285
Number of pages8
JournalAmerican Journal of Kidney Diseases
Volume41
Issue number6
DOIs
Publication statusPublished - Jun 1 2003

Fingerprint

Hepatitis B virus
Dialysis
Population
DNA
Hepatitis B Surface Antigens
Viral Load
Confidence Intervals
Viremia

Keywords

  • Chronic hepatitis B virus (HBV) infection
  • Dialysis
  • Fluctuations
  • Hepatitis B virus viremia (HBV DNA)

ASJC Scopus subject areas

  • Nephrology

Cite this

Biological dynamics of hepatitis B virus load in dialysis population. / Fabrizi, Fabrizio; Lunghi, Giovanna; Alongi, Giancarlo; Bisegna, Sergio; Campolo, Gesualdo; Mangano, Stefano; Limido, Aurelio; Pagliari, Bruno; Tettamanzi, Fabio; Ponticelli, Claudio.

In: American Journal of Kidney Diseases, Vol. 41, No. 6, 01.06.2003, p. 1278-1285.

Research output: Contribution to journalArticle

Fabrizi, F, Lunghi, G, Alongi, G, Bisegna, S, Campolo, G, Mangano, S, Limido, A, Pagliari, B, Tettamanzi, F & Ponticelli, C 2003, 'Biological dynamics of hepatitis B virus load in dialysis population', American Journal of Kidney Diseases, vol. 41, no. 6, pp. 1278-1285. https://doi.org/10.1016/S0272-6386(03)00360-3
Fabrizi, Fabrizio ; Lunghi, Giovanna ; Alongi, Giancarlo ; Bisegna, Sergio ; Campolo, Gesualdo ; Mangano, Stefano ; Limido, Aurelio ; Pagliari, Bruno ; Tettamanzi, Fabio ; Ponticelli, Claudio. / Biological dynamics of hepatitis B virus load in dialysis population. In: American Journal of Kidney Diseases. 2003 ; Vol. 41, No. 6. pp. 1278-1285.
@article{711dadb586b34ee9a34edb8a388462c1,
title = "Biological dynamics of hepatitis B virus load in dialysis population",
abstract = "Background: Control of the spread of hepatitis B virus (HBV) infection in dialysis units has been one of major advances in the management of end-stage renal disease. However, the natural history of HBV in dialysis patients remains unclear. The aim of this study is to measure monthly HBV viral load (HBV DNA) in a large cohort (n = 29) of hepatitis B surface antigen (HBsAg)-positive chronic dialysis patients during 12 months. Methods: HBV DNA was measured using the Amplicor HBV Monitor Test (Roche Diagnostics, Branchburg, NJ), an in vitro assay using polymerase chain reaction nucleic acid amplification and DNA hybridization for the quantitative measurement of HBV DNA in serum. Results: We observed three HBV DNA patterns: (1) patients persistently positive by Amplicor HBV Monitor Test (persistent HBV DNA; 7 of 29 patients; 24.1{\%}), (2) individuals with alternatively positive and negative results (intermittent HBV DNA; 18 of 29 patients; 62.1{\%}), and (3) patients persistently negative by Amplicor HBV Monitor Test (4 of 29 patients; 13.8{\%}). HBV viral load was greater in patients with persistent compared with intermittent HBV DNA (persistently HBV DNA positive; 2.686 × 104 copies/mL; 95{\%} confidence interval [CI], 5.2499 × 104 to 1.8158 × 104 copies/mL) versus intermittently HBV DNA positive (1.071 × 103 copies/mL; 95{\%} CI, 8.524 × 103 to 4.09 × 102 copies/mL; P = 0.0001). In the entire group, HBV load at study entry was low and did not change versus the end of follow-up. Conclusion: Three patterns of HBV viremia in dialysis patients over time were assessed; HBV load was not high and was relatively stable. HBsAg-positive patients who were intermittently HBV DNA positive had less HBV viral load than persistently HBV DNA-positive patients. Periodic testing for HBV DNA to assess the virological status of HBsAg-positive dialysis patients is recommended.",
keywords = "Chronic hepatitis B virus (HBV) infection, Dialysis, Fluctuations, Hepatitis B virus viremia (HBV DNA)",
author = "Fabrizio Fabrizi and Giovanna Lunghi and Giancarlo Alongi and Sergio Bisegna and Gesualdo Campolo and Stefano Mangano and Aurelio Limido and Bruno Pagliari and Fabio Tettamanzi and Claudio Ponticelli",
year = "2003",
month = "6",
day = "1",
doi = "10.1016/S0272-6386(03)00360-3",
language = "English",
volume = "41",
pages = "1278--1285",
journal = "American Journal of Kidney Diseases",
issn = "0272-6386",
publisher = "W.B. Saunders Ltd",
number = "6",

}

TY - JOUR

T1 - Biological dynamics of hepatitis B virus load in dialysis population

AU - Fabrizi, Fabrizio

AU - Lunghi, Giovanna

AU - Alongi, Giancarlo

AU - Bisegna, Sergio

AU - Campolo, Gesualdo

AU - Mangano, Stefano

AU - Limido, Aurelio

AU - Pagliari, Bruno

AU - Tettamanzi, Fabio

AU - Ponticelli, Claudio

PY - 2003/6/1

Y1 - 2003/6/1

N2 - Background: Control of the spread of hepatitis B virus (HBV) infection in dialysis units has been one of major advances in the management of end-stage renal disease. However, the natural history of HBV in dialysis patients remains unclear. The aim of this study is to measure monthly HBV viral load (HBV DNA) in a large cohort (n = 29) of hepatitis B surface antigen (HBsAg)-positive chronic dialysis patients during 12 months. Methods: HBV DNA was measured using the Amplicor HBV Monitor Test (Roche Diagnostics, Branchburg, NJ), an in vitro assay using polymerase chain reaction nucleic acid amplification and DNA hybridization for the quantitative measurement of HBV DNA in serum. Results: We observed three HBV DNA patterns: (1) patients persistently positive by Amplicor HBV Monitor Test (persistent HBV DNA; 7 of 29 patients; 24.1%), (2) individuals with alternatively positive and negative results (intermittent HBV DNA; 18 of 29 patients; 62.1%), and (3) patients persistently negative by Amplicor HBV Monitor Test (4 of 29 patients; 13.8%). HBV viral load was greater in patients with persistent compared with intermittent HBV DNA (persistently HBV DNA positive; 2.686 × 104 copies/mL; 95% confidence interval [CI], 5.2499 × 104 to 1.8158 × 104 copies/mL) versus intermittently HBV DNA positive (1.071 × 103 copies/mL; 95% CI, 8.524 × 103 to 4.09 × 102 copies/mL; P = 0.0001). In the entire group, HBV load at study entry was low and did not change versus the end of follow-up. Conclusion: Three patterns of HBV viremia in dialysis patients over time were assessed; HBV load was not high and was relatively stable. HBsAg-positive patients who were intermittently HBV DNA positive had less HBV viral load than persistently HBV DNA-positive patients. Periodic testing for HBV DNA to assess the virological status of HBsAg-positive dialysis patients is recommended.

AB - Background: Control of the spread of hepatitis B virus (HBV) infection in dialysis units has been one of major advances in the management of end-stage renal disease. However, the natural history of HBV in dialysis patients remains unclear. The aim of this study is to measure monthly HBV viral load (HBV DNA) in a large cohort (n = 29) of hepatitis B surface antigen (HBsAg)-positive chronic dialysis patients during 12 months. Methods: HBV DNA was measured using the Amplicor HBV Monitor Test (Roche Diagnostics, Branchburg, NJ), an in vitro assay using polymerase chain reaction nucleic acid amplification and DNA hybridization for the quantitative measurement of HBV DNA in serum. Results: We observed three HBV DNA patterns: (1) patients persistently positive by Amplicor HBV Monitor Test (persistent HBV DNA; 7 of 29 patients; 24.1%), (2) individuals with alternatively positive and negative results (intermittent HBV DNA; 18 of 29 patients; 62.1%), and (3) patients persistently negative by Amplicor HBV Monitor Test (4 of 29 patients; 13.8%). HBV viral load was greater in patients with persistent compared with intermittent HBV DNA (persistently HBV DNA positive; 2.686 × 104 copies/mL; 95% confidence interval [CI], 5.2499 × 104 to 1.8158 × 104 copies/mL) versus intermittently HBV DNA positive (1.071 × 103 copies/mL; 95% CI, 8.524 × 103 to 4.09 × 102 copies/mL; P = 0.0001). In the entire group, HBV load at study entry was low and did not change versus the end of follow-up. Conclusion: Three patterns of HBV viremia in dialysis patients over time were assessed; HBV load was not high and was relatively stable. HBsAg-positive patients who were intermittently HBV DNA positive had less HBV viral load than persistently HBV DNA-positive patients. Periodic testing for HBV DNA to assess the virological status of HBsAg-positive dialysis patients is recommended.

KW - Chronic hepatitis B virus (HBV) infection

KW - Dialysis

KW - Fluctuations

KW - Hepatitis B virus viremia (HBV DNA)

UR - http://www.scopus.com/inward/record.url?scp=12444333590&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=12444333590&partnerID=8YFLogxK

U2 - 10.1016/S0272-6386(03)00360-3

DO - 10.1016/S0272-6386(03)00360-3

M3 - Article

C2 - 12776281

AN - SCOPUS:12444333590

VL - 41

SP - 1278

EP - 1285

JO - American Journal of Kidney Diseases

JF - American Journal of Kidney Diseases

SN - 0272-6386

IS - 6

ER -