Biological effects of ultrasound stimulus on cells derived from human ovarian follicular liquid

Claudia Omes; Lorenzo Fassina; Giovanni Magenes; Daniela Ogliari; Carmine Tinelli; Federica Riva

doi:10.1109/EMBC.2013.6609634

Biological effects of ultrasound stimulus on cells derived from human ovarian follicular liquid

Claudia Omes, Lorenzo Fassina, Giovanni Magenes, Daniela Ogliari, Carmine Tinelli, Federica Riva

IRCCS Fondazione Policlinico San Matteo

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

Low-Intensity Pulsed Ultrasound Stimulus (LIPUS) accelerates the bone fracture healing in animal models and in clinical studies. In this work, according to the literature, we have chosen the mesenchymal stem cells (MSCs) as precursors of bony tissue, in particular the MSCs derived from the human ovarian follicular liquid (FL), and we have investigated the effects of ultrasounds on their proliferation. We tested two different durations of ultrasound stimulus (2 and 5 min) and compared these data to the control without ultrasound treatment. To quantify the proliferation of these putative MSCs, we used the BrdU incorporation assay: in comparison with the control, the results showed that 5 min of ultrasound stimulus significantly increased the percentage number of cells in intensive proliferative activity; on the other hand, there was no significant difference using 2 min of stimulation, hypothetically because the transmitted energy was not sufficient to stimulate the cells and to consequently enhance their proliferation. In conclusion, the effects of LIPUS on putative MSCs derived from ovarian follicular liquid show potential developments in biotech or medical applications.

Original language	English
Title of host publication	Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS
Pages	850-853
Number of pages	4
DOIs	https://doi.org/10.1109/EMBC.2013.6609634
Publication status	Published - 2013
Event	2013 35th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2013 - Osaka, Japan Duration: Jul 3 2013 → Jul 7 2013

Other

Other	2013 35th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2013
Country	Japan
City	Osaka
Period	7/3/13 → 7/7/13

ASJC Scopus subject areas

Computer Vision and Pattern Recognition
Signal Processing
Biomedical Engineering
Health Informatics

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Biological effects of ultrasound stimulus on cells derived from human ovarian follicular liquid. / Omes, Claudia; Fassina, Lorenzo; Magenes, Giovanni; Ogliari, Daniela; Tinelli, Carmine; Riva, Federica.

Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS. 2013. p. 850-853 6609634.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Omes, C, Fassina, L, Magenes, G, Ogliari, D, Tinelli, C & Riva, F 2013, Biological effects of ultrasound stimulus on cells derived from human ovarian follicular liquid. in Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS., 6609634, pp. 850-853, 2013 35th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2013, Osaka, Japan, 7/3/13. https://doi.org/10.1109/EMBC.2013.6609634

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abstract = "Low-Intensity Pulsed Ultrasound Stimulus (LIPUS) accelerates the bone fracture healing in animal models and in clinical studies. In this work, according to the literature, we have chosen the mesenchymal stem cells (MSCs) as precursors of bony tissue, in particular the MSCs derived from the human ovarian follicular liquid (FL), and we have investigated the effects of ultrasounds on their proliferation. We tested two different durations of ultrasound stimulus (2 and 5 min) and compared these data to the control without ultrasound treatment. To quantify the proliferation of these putative MSCs, we used the BrdU incorporation assay: in comparison with the control, the results showed that 5 min of ultrasound stimulus significantly increased the percentage number of cells in intensive proliferative activity; on the other hand, there was no significant difference using 2 min of stimulation, hypothetically because the transmitted energy was not sufficient to stimulate the cells and to consequently enhance their proliferation. In conclusion, the effects of LIPUS on putative MSCs derived from ovarian follicular liquid show potential developments in biotech or medical applications.",

author = "Claudia Omes and Lorenzo Fassina and Giovanni Magenes and Daniela Ogliari and Carmine Tinelli and Federica Riva",

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N2 - Low-Intensity Pulsed Ultrasound Stimulus (LIPUS) accelerates the bone fracture healing in animal models and in clinical studies. In this work, according to the literature, we have chosen the mesenchymal stem cells (MSCs) as precursors of bony tissue, in particular the MSCs derived from the human ovarian follicular liquid (FL), and we have investigated the effects of ultrasounds on their proliferation. We tested two different durations of ultrasound stimulus (2 and 5 min) and compared these data to the control without ultrasound treatment. To quantify the proliferation of these putative MSCs, we used the BrdU incorporation assay: in comparison with the control, the results showed that 5 min of ultrasound stimulus significantly increased the percentage number of cells in intensive proliferative activity; on the other hand, there was no significant difference using 2 min of stimulation, hypothetically because the transmitted energy was not sufficient to stimulate the cells and to consequently enhance their proliferation. In conclusion, the effects of LIPUS on putative MSCs derived from ovarian follicular liquid show potential developments in biotech or medical applications.

AB - Low-Intensity Pulsed Ultrasound Stimulus (LIPUS) accelerates the bone fracture healing in animal models and in clinical studies. In this work, according to the literature, we have chosen the mesenchymal stem cells (MSCs) as precursors of bony tissue, in particular the MSCs derived from the human ovarian follicular liquid (FL), and we have investigated the effects of ultrasounds on their proliferation. We tested two different durations of ultrasound stimulus (2 and 5 min) and compared these data to the control without ultrasound treatment. To quantify the proliferation of these putative MSCs, we used the BrdU incorporation assay: in comparison with the control, the results showed that 5 min of ultrasound stimulus significantly increased the percentage number of cells in intensive proliferative activity; on the other hand, there was no significant difference using 2 min of stimulation, hypothetically because the transmitted energy was not sufficient to stimulate the cells and to consequently enhance their proliferation. In conclusion, the effects of LIPUS on putative MSCs derived from ovarian follicular liquid show potential developments in biotech or medical applications.

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