Biological monitoring of exposure to tebuconazole in winegrowers

Silvia Fustinoni, Rosa Mercadante, Elisa Polledri, Federico Maria Rubino, Stefan Mandic-Rajcevic, Giorgio Vianello, Claudio Colosio, Angelo Moretto

Research output: Contribution to journalArticlepeer-review


Tebuconazole (TEB) is a fungicide widely used in vineyards and is a suspected teratogen for humans. The aim of this research was to identify urinary biomarkers an. The best sampling time fo. The biological monitoring of exposure to TEB in agricultural workers. Seven vineyard workers o. The Monferrato region, Piedemont, Italy, were investigated for a total of 12 workdays. They treate. The vineyards with TEB for 1-2 consecutive days, one of them for 3 days. During each application coveralls, underwears, hand washing liquids and head coverings were used to estimate dermal exposure. For biomonitoring, spot samples of urine from each individual were collected starting from 24 h befor. The first application, continuing durin. The application, and again afte. The application for about 48 h. TEB and its metabolites TEB-OH and TEB-COOH were measured by liquid chromatography/triple quadrupole mass spectrometry. TEB contamination of coveralls and total dermal exposure showed median levels of 6180 and 1020 μg. Urinary TEB-OH wa. The most abundant metabolite; its excretion rate peaked within 24 h after product application (post 24 h). In this time frame, median levels of TEB-OH and TEB-COOH ranged from 8.0 to 387.8 μg/l and from 5.7 to 102.9 μg/l, respectively, with a ratio betwee. The two metabolites of about 3.5. The total amount of urinary metabolites (U-TEB eq) post 24 h was significantly correlated with both TEB on coveralls and total dermal exposure (Pearson's r=0.756 and 0.577). The amount of metabolites excreted in urine represented about 17% of total dermal TEB exposure. Our results suggest that TEB-OH and TEB-COOH in post-exposure urine samples are promising candidates for biomonitoring TEB exposure in agricultural workers.

Original languageEnglish
Pages (from-to)643-649
Number of pages7
JournalJournal of Exposure Science and Environmental Epidemiology
Issue number6
Publication statusPublished - Nov 25 2014


  • biomonitoring
  • carboxy-tebuconazole
  • hydroxy-tebuconazole
  • pesticide application
  • tebuconazole metabolite
  • urine

ASJC Scopus subject areas

  • Pollution
  • Public Health, Environmental and Occupational Health
  • Toxicology
  • Epidemiology
  • Medicine(all)


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