Biological monitoring of human exposure to coal tar - Urinary excretion of total polycyclic aromatic hydrocarbons, 1-hydroxypyrene and mutagens in psoriatic patients

Erminio Clonfero, Mauro Zordan, Paola Venier, Maurizio Paleologo, Angelo G. Levis, Danilo Cottica, Luigi Pozzoli, Frans J. Jongeneelen, Robert P. Bos, Robert B M Anzion

Research output: Contribution to journalArticle

Abstract

Three methods for the biological monitoring of human exposure to coal tar were compared. Levels of 1-hydroxypyrene(1-OH PYR), polycyclic aromatic hydrocarbons (PAH) and mutagens (Ames plate incorporation assay using Salmonella typhimurium strain TA98 in the presence of S9 and β-glucuronidase) were determined in urinary samples from psoriatic patients undergoing topical treatment with mineral coal tar. A single sample of urine with a high content of PAH was diluted with urine of non-exposed, non-smoking subjects in order to obtain nine samples with a decreasing content of PAH metabolites. Mutagenicity of the extracts was detectable down to the dilution corresponding to a content in 1-OH PYR of about 50 μg/g creatinine and total PAH of 7 μg/g creatinine. In a second phase the three indicators of exposure to PAH were compared in 16 urinary samples from four psoriatic patients. The total PAH levels determined by the acidic deconjugation/reduction method were confirmed to be nearly always lower than the corresponding levels of 1-OH PYR alone. Most of the extracts were mutagenic, however, some of the samples with a high content in PAH metabolites were not mutagenic. In all the urinary samples analyzed the excretion of 1-OH PYR was markedly greater than in control subjects. 1-OH PYR and urinary mutagenicity levels were well correlated. The present data suggest that both the determination of mutagenicity and 1-OH PYR in urine may be used to monitor occupational exposure to PAH, the latter method being cheaper and of greater specificity and sensitivity.

Original languageEnglish
Pages (from-to)363-368
Number of pages6
JournalInternational Archives of Occupational and Environmental Health
Volume61
Issue number6
DOIs
Publication statusPublished - Jun 1989

Keywords

  • 1-Hydroxypyrene
  • Ames test
  • Polycyclic aromatic hydrocarbons
  • Psoriasis
  • Urine

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health
  • Health, Toxicology and Mutagenesis

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