TY - JOUR
T1 - Biological role of NHERF1 protein expression in breast cancer
AU - Mangia, Anita
AU - Chiriatti, Annalisa
AU - Bellizzi, Antonia
AU - Malfettone, Andrea
AU - Stea, Baldassarre
AU - Zito, Francesso Alfredo
AU - Reshkin, Stephen Joel
AU - Simone, Giovanni
AU - Paradiso, Angelo
PY - 2009/11
Y1 - 2009/11
N2 - Aims: To determine the role of Na+H+ exchanger regulatory factor (NHERF1) in breast cancerogenesis and progression. Methods and results: NHERF1 expression was examined in normal tissue, ductal carcinoma in situ (DCIS), invasive carcinoma (IBC), synchronous metastatic lymph node and metachronous distant metastases of a retrospective series of breast cancers. Fifty-one IBC, 42 DCIS and normal tissues were examined immunohistochemically, and the colocalization between NHERF1 and HER2neu was studied by immunofluorescence. NHERF1 showed a different localization and pattern of expression in the different compartments of the breast. The mean value of cytoplasmic NHERF1 expression in paired samples was significantly higher in DCIS, IBC, distant metastases and metastatic lymph nodes with respect to normal tissues. Moreover, in metastatic lymph nodes NHERF1 was exclusively cytoplasmic. In the membrane NHERF1 was colocalized with overexpressed HER2neu in DCIS, IBC and distant metastases. Conclusions: Breast cancerogenesis is characterized by increased cytoplasmic expression of NHERF1 as the tumour progresses, suggesting a role in this process. The switch from apical membranous to cytoplasmic expression is compatible with a dual role for NHERF1 as a tumour suppressor or tumour promoter dependent on its subcellular localization.
AB - Aims: To determine the role of Na+H+ exchanger regulatory factor (NHERF1) in breast cancerogenesis and progression. Methods and results: NHERF1 expression was examined in normal tissue, ductal carcinoma in situ (DCIS), invasive carcinoma (IBC), synchronous metastatic lymph node and metachronous distant metastases of a retrospective series of breast cancers. Fifty-one IBC, 42 DCIS and normal tissues were examined immunohistochemically, and the colocalization between NHERF1 and HER2neu was studied by immunofluorescence. NHERF1 showed a different localization and pattern of expression in the different compartments of the breast. The mean value of cytoplasmic NHERF1 expression in paired samples was significantly higher in DCIS, IBC, distant metastases and metastatic lymph nodes with respect to normal tissues. Moreover, in metastatic lymph nodes NHERF1 was exclusively cytoplasmic. In the membrane NHERF1 was colocalized with overexpressed HER2neu in DCIS, IBC and distant metastases. Conclusions: Breast cancerogenesis is characterized by increased cytoplasmic expression of NHERF1 as the tumour progresses, suggesting a role in this process. The switch from apical membranous to cytoplasmic expression is compatible with a dual role for NHERF1 as a tumour suppressor or tumour promoter dependent on its subcellular localization.
KW - Breast cancer
KW - HER2neu
KW - Immunofluorescence
KW - Immunohistochemistry
KW - NHERF1
UR - http://www.scopus.com/inward/record.url?scp=71049145264&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=71049145264&partnerID=8YFLogxK
U2 - 10.1111/j.1365-2559.2009.03424.x
DO - 10.1111/j.1365-2559.2009.03424.x
M3 - Article
C2 - 19912366
AN - SCOPUS:71049145264
VL - 55
SP - 600
EP - 608
JO - Histopathology
JF - Histopathology
SN - 0309-0167
IS - 5
ER -