Screening biologico mediante CFU-GM assay delle insufficienze midollari immuno-mediate.

Translated title of the contribution: Biological screening of immuno-mediated marrow insufficiency by means of the CFU-GM assay

A. Ghizzi, C. Carlo Stella, M. Bonfichi, M. Gorini, P. Pedrazzoli

Research output: Contribution to journalArticle

Abstract

We studied in agar growth behaviour of bone marrow granulocyte-macrophage progenitor cells (CFU-GM) in 16 patients with marrow failure in order to discriminate patients with cells inhibiting granulopoiesis both in bone marrow and peripheral blood. Our experimental design was based on: a) agar culture of bone marrow cells before and after treatment with antilymphocyte globulin (AGL); b) agar co-culture of marrow cells with autologous lymphocytes from peripheral blood. ALG treatment of marrow cells determined CFU-GM growth increase in 4 out of 10 patients; in the same patients co-culture with autologous lymphocytes showed a significant inhibition of CFU-GM growth. The growth enhancement induced by ALG treatment "in vitro" associated with growth inhibition in the coculture suggests the existence of a lymphocyte population suppressing the granulopoiesis both in bone marrow and peripheral blood. With this work we propose an experimental model in order to discriminate marrow failure based on cell-mediated suppression of CFU-GM growth in patient susceptible of immunosuppressive therapy with ALG.

Original languageItalian
Pages (from-to)2179-2185
Number of pages7
JournalBollettino della Societa Italiana di Biologia Sperimentale
Volume60
Issue number11
Publication statusPublished - Nov 30 1984

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Granulocyte-Macrophage Progenitor Cells
Bone Marrow
Growth
Coculture Techniques
Agar
Lymphocytes
Antilymphocyte Serum
Therapeutics
Immunosuppressive Agents
Bone Marrow Cells
Research Design
Theoretical Models
Population

ASJC Scopus subject areas

  • Medicine(all)

Cite this

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abstract = "We studied in agar growth behaviour of bone marrow granulocyte-macrophage progenitor cells (CFU-GM) in 16 patients with marrow failure in order to discriminate patients with cells inhibiting granulopoiesis both in bone marrow and peripheral blood. Our experimental design was based on: a) agar culture of bone marrow cells before and after treatment with antilymphocyte globulin (AGL); b) agar co-culture of marrow cells with autologous lymphocytes from peripheral blood. ALG treatment of marrow cells determined CFU-GM growth increase in 4 out of 10 patients; in the same patients co-culture with autologous lymphocytes showed a significant inhibition of CFU-GM growth. The growth enhancement induced by ALG treatment {"}in vitro{"} associated with growth inhibition in the coculture suggests the existence of a lymphocyte population suppressing the granulopoiesis both in bone marrow and peripheral blood. With this work we propose an experimental model in order to discriminate marrow failure based on cell-mediated suppression of CFU-GM growth in patient susceptible of immunosuppressive therapy with ALG.",
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AU - Carlo Stella, C.

AU - Bonfichi, M.

AU - Gorini, M.

AU - Pedrazzoli, P.

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