Bioluminescent flow sensors: L-alanine determination in serum and urine

S. Girotti, A. Roda, A. L. Piacentini, M. A. Angellotti, S. Ghini, S. Piazzi, G. Carrea, R. Bovara

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

A continuous flow bioluminescent method for L-alanine analysis in serum and urine has been developed. Serum can be analyzed directly after simple filtration. Response is linear from 50 to 1500 pmoles in biological matrix. Alanine dehydrogenase is immobilized onto a nylon coil separated from the reactor coil containing bioluminescent enzymes. The stability of nylon immobilized enzymes is high (over three months) and more than 900 samples can be analyzed with few mg of enzymes. The results obtained with the bioluminescent sensor agree well with those obtained by ion exchange chromatography (amino acid analyzer).

Original languageEnglish
Pages (from-to)1315-1330
Number of pages16
JournalAnalytical Letters
Volume20
Issue number8
DOIs
Publication statusPublished - 1987

Fingerprint

Nylons
Alanine
Alanine Dehydrogenase
Enzymes
Urine
Immobilized Enzymes
Sensors
Ion Exchange Chromatography
Chromatography
Serum
Ion exchange
Amino Acids
Amino acids

Keywords

  • Bacterial bioluminescence
  • determination
  • flow sensors
  • immobilized enzymes
  • L-alanine

ASJC Scopus subject areas

  • Analytical Chemistry
  • Spectroscopy
  • Electrochemistry
  • Biochemistry, medical
  • Clinical Biochemistry
  • Biochemistry
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Girotti, S., Roda, A., Piacentini, A. L., Angellotti, M. A., Ghini, S., Piazzi, S., ... Bovara, R. (1987). Bioluminescent flow sensors: L-alanine determination in serum and urine. Analytical Letters, 20(8), 1315-1330. https://doi.org/10.1080/00032718708064611

Bioluminescent flow sensors : L-alanine determination in serum and urine. / Girotti, S.; Roda, A.; Piacentini, A. L.; Angellotti, M. A.; Ghini, S.; Piazzi, S.; Carrea, G.; Bovara, R.

In: Analytical Letters, Vol. 20, No. 8, 1987, p. 1315-1330.

Research output: Contribution to journalArticle

Girotti, S, Roda, A, Piacentini, AL, Angellotti, MA, Ghini, S, Piazzi, S, Carrea, G & Bovara, R 1987, 'Bioluminescent flow sensors: L-alanine determination in serum and urine', Analytical Letters, vol. 20, no. 8, pp. 1315-1330. https://doi.org/10.1080/00032718708064611
Girotti S, Roda A, Piacentini AL, Angellotti MA, Ghini S, Piazzi S et al. Bioluminescent flow sensors: L-alanine determination in serum and urine. Analytical Letters. 1987;20(8):1315-1330. https://doi.org/10.1080/00032718708064611
Girotti, S. ; Roda, A. ; Piacentini, A. L. ; Angellotti, M. A. ; Ghini, S. ; Piazzi, S. ; Carrea, G. ; Bovara, R. / Bioluminescent flow sensors : L-alanine determination in serum and urine. In: Analytical Letters. 1987 ; Vol. 20, No. 8. pp. 1315-1330.
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