Biomarkers for Monitoring Pre-Analytical Quality Variation of mRNA in Blood Samples

Hui Zhang, Vlasta Korenková, Robert Sjöback, David Švec, Jens Björkman, Mogens Kruhoffer, Paolo Verderio, Sara Pizzamiglio, Chiara Maura Ciniselli, Ralf Wyrich, Uwe Oelmueller, Mikael Kubista, Torbjorn Lindahl, Anders Lönneborg, Edith Rian

Research output: Contribution to journalArticle

Abstract

There is an increasing need for proper quality control tools in the pre-analytical phase of the molecular diagnostic workflow. The aim of the present study was to identify biomarkers for monitoring pre-analytical mRNA quality variations in two different types of blood collection tubes, K2EDTA (EDTA) tubes and PAXgene Blood RNA Tubes (PAXgene tubes). These tubes are extensively used both in the diagnostic setting as well as for research biobank samples. Blood specimens collected in the two different blood collection tubes were stored for varying times at different temperatures, and microarray analysis was performed on resultant extracted RNA. A large set of potential mRNA quality biomarkers for monitoring postphlebotomy gene expression changes and mRNA degradation in blood was identified. qPCR assays for the potential biomarkers and a set of relevant reference genes were generated and used to pre-validate a sub-set of the selected biomarkers. The assay precision of the potential qPCR based biomarkers was determined, and a final validation of the selected quality biomarkers using the developed qPCR assays and blood samples from 60 healthy additional subjects was performed. In total, four mRNA quality biomarkers (USP32, LMNA, FOSB, TNRFSF10C) were successfully validated. We suggest here the use of these blood mRNA quality biomarkers for validating an experimental pre-analytical workflow. These biomarkers were further evaluated in the 2nd ring trial of the SPIDIA-RNA Program which demonstrated that these biomarkers can be used as quality control tools for mRNA analyses from blood samples. Ci.

Original languageEnglish
Article numbere111644
JournalPLoS One
Volume9
Issue number11
DOIs
Publication statusPublished - Nov 1 2014

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Biomarkers
biomarkers
Blood
Messenger RNA
Monitoring
monitoring
blood
sampling
Assays
Workflow
RNA
Quality Control
quality control
Quality control
assays
Molecular Pathology
blood groups
RNA Precursors
RNA Stability
Gene Expression Profiling

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Zhang, H., Korenková, V., Sjöback, R., Švec, D., Björkman, J., Kruhoffer, M., ... Rian, E. (2014). Biomarkers for Monitoring Pre-Analytical Quality Variation of mRNA in Blood Samples. PLoS One, 9(11), [e111644]. https://doi.org/10.1371/journal.pone.0111644

Biomarkers for Monitoring Pre-Analytical Quality Variation of mRNA in Blood Samples. / Zhang, Hui; Korenková, Vlasta; Sjöback, Robert; Švec, David; Björkman, Jens; Kruhoffer, Mogens; Verderio, Paolo; Pizzamiglio, Sara; Ciniselli, Chiara Maura; Wyrich, Ralf; Oelmueller, Uwe; Kubista, Mikael; Lindahl, Torbjorn; Lönneborg, Anders; Rian, Edith.

In: PLoS One, Vol. 9, No. 11, e111644, 01.11.2014.

Research output: Contribution to journalArticle

Zhang, H, Korenková, V, Sjöback, R, Švec, D, Björkman, J, Kruhoffer, M, Verderio, P, Pizzamiglio, S, Ciniselli, CM, Wyrich, R, Oelmueller, U, Kubista, M, Lindahl, T, Lönneborg, A & Rian, E 2014, 'Biomarkers for Monitoring Pre-Analytical Quality Variation of mRNA in Blood Samples', PLoS One, vol. 9, no. 11, e111644. https://doi.org/10.1371/journal.pone.0111644
Zhang H, Korenková V, Sjöback R, Švec D, Björkman J, Kruhoffer M et al. Biomarkers for Monitoring Pre-Analytical Quality Variation of mRNA in Blood Samples. PLoS One. 2014 Nov 1;9(11). e111644. https://doi.org/10.1371/journal.pone.0111644
Zhang, Hui ; Korenková, Vlasta ; Sjöback, Robert ; Švec, David ; Björkman, Jens ; Kruhoffer, Mogens ; Verderio, Paolo ; Pizzamiglio, Sara ; Ciniselli, Chiara Maura ; Wyrich, Ralf ; Oelmueller, Uwe ; Kubista, Mikael ; Lindahl, Torbjorn ; Lönneborg, Anders ; Rian, Edith. / Biomarkers for Monitoring Pre-Analytical Quality Variation of mRNA in Blood Samples. In: PLoS One. 2014 ; Vol. 9, No. 11.
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