Biomechanical and angiogenic properties of tissue-engineered rat trachea using genipin cross-linked decellularized tissue

Johannes Haag, Silvia Baiguera, Philipp Jungebluth, Daniel Barale, Costantino Del Gaudio, Francesca Castiglione, Alessandra Bianco, Camilla E. Comin, Domenico Ribatti, Paolo Macchiarini

Research output: Contribution to journalArticlepeer-review

Abstract

In this study, the obtainment and characterization of decellularized rat tracheal grafts are described. The detergent-enzymatic method, already used to develop bioengineered pig and human trachea scaffolds, has been applied to rat tracheae in order to obtain airway grafts suitable to be used to improve our knowledge on the process of tissue-engineered airway transplantation and regeneration. The results demonstrated that, after 9 detergent-enzymatic cycles, almost complete decellularized tracheae, retaining the hierarchical and mechanical properties of the native tissues with strong in vivo angiogenic characteristics, could be obtained. Moreover, to improve the mechanical properties of decellularized tracheae, genipin is here considered as a naturally derived cross-linking agent. The results demonstrated that the treatment increased mechanical properties, in term of secant modulus, without neither altering the pro-angiogenic properties of decellularized airway matrices or eliciting an in vivo inflammatory response.

Original languageEnglish
Pages (from-to)780-789
Number of pages10
JournalBiomaterials
Volume33
Issue number3
DOIs
Publication statusPublished - Jan 2012

Keywords

  • Angiogenesis
  • Animal model
  • Cross-linking
  • Decellularized trachea
  • Mechanical properties
  • Tissue engineering

ASJC Scopus subject areas

  • Biomaterials
  • Bioengineering
  • Ceramics and Composites
  • Mechanics of Materials
  • Biophysics

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