Biomimetic Concealing of PLGA Microspheres in a 3D Scaffold to Prevent Macrophage Uptake

Silvia Minardi, Bruna Corradetti, Francesca Taraballi, Monica Sandri, Jonathan O. Martinez, Sebastian T. Powell, Anna Tampieri, Bradley K. Weiner, Ennio Tasciotti

Research output: Contribution to journalArticlepeer-review

Abstract

Scaffolds functionalized with delivery systems for the release of growth factors is a robust strategy to enhance tissue regeneration. However, after implantation, macrophages infiltrate the scaffold, eventually initiating the degradation and clearance of the delivery systems. Herein, it is hypothesized that fully embedding the poly(d,l-lactide-co-glycolide acid) microspheres (MS) in a highly structured collagen-based scaffold (concealing) can prevent their detection, preserving the integrity of the payload. Confocal laser microscopy reveals that non-embedded MS are easily internalized; when concealed, J774 and bone marrow-derived macrophages (BMDM) cannot detect them. This is further demonstrated by flow cytometry, as a tenfold decrease is found in the number of MS engulfed by the cells, suggesting that collagen can cloak the MS. This correlates with the amount of nitric oxide and tumor necrosis factor-α produced by J774 and BMDM in response to the concealed MS, comparable to that found for non-functionalized collagen scaffolds. Finally, the release kinetics of a reporter protein is preserved in the presence of macrophages, only when MS are concealed. The data provide detailed strategies for fabricating three dimensional (3D) biomimetic scaffolds able to conceal delivery systems and preserve the therapeutic molecules for release.

Original languageEnglish
JournalSmall
DOIs
Publication statusAccepted/In press - 2016

Keywords

  • Biomimetic
  • Controlled release
  • Delivery systems
  • Scaffolds
  • Tissue engineering

ASJC Scopus subject areas

  • Biomaterials
  • Engineering (miscellaneous)
  • Biotechnology

Fingerprint Dive into the research topics of 'Biomimetic Concealing of PLGA Microspheres in a 3D Scaffold to Prevent Macrophage Uptake'. Together they form a unique fingerprint.

Cite this