Biomimetic modification of synthetic hydrogels by incorporation of adhesive peptides and calcium phosphate nanoparticles: In vitro evaluation of cell behavior

M. Bongio, J. J J P van den Beucken, M. R. Nejadnik, S. C G Leeuwenburgh, L. A. Kinard, F. K. Kasper, A. G. Mikos, J. A. Jansen

Research output: Contribution to journalArticle

Abstract

The ultimate goal of this work was to develop a biocompatible and biomimetic in situ crosslinkable hydrogel scaffold with an instructive capacity for bone regenerative treatment. To this end, synthetic hydrogels were functionalized with two key components of the extracellular matrix of native bone tissue, i.e. the three-amino acid peptide sequence RGD (which is the principal integrin-binding domain responsible for cell adhesion and survival of anchorage-dependent cells) and calcium phosphate (CaP) nanoparticles in the form of hydroxyapatite (which are similar to the inorganic phase of bone tissue). Rat bone marrow osteoblast-like cells (OBLCs) were encapsulated in four different biomaterials (plain oligo(poly(ethylene glycol) fumarate) (OPF), RGD-modified OPF, OPF enriched with CaP nanoparticles and RGD-modified OPF enriched with CaP nanoparticles) and cell survival, cell spreading, proliferation and mineralized matrix formation were determined via cell viability assay, histology and biochemical analysis for alkaline phosphatase activity and calcium. This study showed that RGD peptide sequences promoted cell spreading in OPF hydrogels and hence play a crucial role in cell survival during the early stage of culture, whereas CaP nanoparticles significantly enhanced cell-mediated hydrogel mineralization. Although cell spreading and proliferation activity were inhibited, the combined effect of RGD peptide sequences and CaP nanoparticles within OPF hydrogel systems elicited a better biological response than that of the individual components. Specifically, both a sustained cell viability and mineralized matrix production mediated by encapsulated OBLCs were observed within these novel biomimetic composite systems.

Original languageEnglish
Pages (from-to)359-376
Number of pages18
JournalEuropean Cells and Materials
Volume22
Publication statusPublished - Dec 2011

Fingerprint

Biomimetics
Hydrogels
Calcium phosphate
Adhesives
Nanoparticles
Peptides
Cell Survival
Cells
Hydrogel
Bone
Osteoblasts
Bone and Bones
Cell Proliferation
Tissue
Histology
Cell adhesion
Phosphatases
Cell proliferation
Biocompatible Materials
Durapatite

Keywords

  • Biomimetic hydrogels
  • Calcium phosphate nanoparticles
  • Cell-material interactions
  • In vitro mineralization
  • Rgd peptide
  • Synthetic polymers

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Bioengineering
  • Biomedical Engineering
  • Biomaterials
  • Medicine(all)

Cite this

Bongio, M., van den Beucken, J. J. J. P., Nejadnik, M. R., Leeuwenburgh, S. C. G., Kinard, L. A., Kasper, F. K., ... Jansen, J. A. (2011). Biomimetic modification of synthetic hydrogels by incorporation of adhesive peptides and calcium phosphate nanoparticles: In vitro evaluation of cell behavior. European Cells and Materials, 22, 359-376.

Biomimetic modification of synthetic hydrogels by incorporation of adhesive peptides and calcium phosphate nanoparticles : In vitro evaluation of cell behavior. / Bongio, M.; van den Beucken, J. J J P; Nejadnik, M. R.; Leeuwenburgh, S. C G; Kinard, L. A.; Kasper, F. K.; Mikos, A. G.; Jansen, J. A.

In: European Cells and Materials, Vol. 22, 12.2011, p. 359-376.

Research output: Contribution to journalArticle

Bongio, M, van den Beucken, JJJP, Nejadnik, MR, Leeuwenburgh, SCG, Kinard, LA, Kasper, FK, Mikos, AG & Jansen, JA 2011, 'Biomimetic modification of synthetic hydrogels by incorporation of adhesive peptides and calcium phosphate nanoparticles: In vitro evaluation of cell behavior', European Cells and Materials, vol. 22, pp. 359-376.
Bongio, M. ; van den Beucken, J. J J P ; Nejadnik, M. R. ; Leeuwenburgh, S. C G ; Kinard, L. A. ; Kasper, F. K. ; Mikos, A. G. ; Jansen, J. A. / Biomimetic modification of synthetic hydrogels by incorporation of adhesive peptides and calcium phosphate nanoparticles : In vitro evaluation of cell behavior. In: European Cells and Materials. 2011 ; Vol. 22. pp. 359-376.
@article{06007c0bdf074e59aefafc8701de4b1d,
title = "Biomimetic modification of synthetic hydrogels by incorporation of adhesive peptides and calcium phosphate nanoparticles: In vitro evaluation of cell behavior",
abstract = "The ultimate goal of this work was to develop a biocompatible and biomimetic in situ crosslinkable hydrogel scaffold with an instructive capacity for bone regenerative treatment. To this end, synthetic hydrogels were functionalized with two key components of the extracellular matrix of native bone tissue, i.e. the three-amino acid peptide sequence RGD (which is the principal integrin-binding domain responsible for cell adhesion and survival of anchorage-dependent cells) and calcium phosphate (CaP) nanoparticles in the form of hydroxyapatite (which are similar to the inorganic phase of bone tissue). Rat bone marrow osteoblast-like cells (OBLCs) were encapsulated in four different biomaterials (plain oligo(poly(ethylene glycol) fumarate) (OPF), RGD-modified OPF, OPF enriched with CaP nanoparticles and RGD-modified OPF enriched with CaP nanoparticles) and cell survival, cell spreading, proliferation and mineralized matrix formation were determined via cell viability assay, histology and biochemical analysis for alkaline phosphatase activity and calcium. This study showed that RGD peptide sequences promoted cell spreading in OPF hydrogels and hence play a crucial role in cell survival during the early stage of culture, whereas CaP nanoparticles significantly enhanced cell-mediated hydrogel mineralization. Although cell spreading and proliferation activity were inhibited, the combined effect of RGD peptide sequences and CaP nanoparticles within OPF hydrogel systems elicited a better biological response than that of the individual components. Specifically, both a sustained cell viability and mineralized matrix production mediated by encapsulated OBLCs were observed within these novel biomimetic composite systems.",
keywords = "Biomimetic hydrogels, Calcium phosphate nanoparticles, Cell-material interactions, In vitro mineralization, Rgd peptide, Synthetic polymers",
author = "M. Bongio and {van den Beucken}, {J. J J P} and Nejadnik, {M. R.} and Leeuwenburgh, {S. C G} and Kinard, {L. A.} and Kasper, {F. K.} and Mikos, {A. G.} and Jansen, {J. A.}",
year = "2011",
month = "12",
language = "English",
volume = "22",
pages = "359--376",
journal = "European Cells and Materials",
issn = "1473-2262",
publisher = "Swiss Society for Biomaterials",

}

TY - JOUR

T1 - Biomimetic modification of synthetic hydrogels by incorporation of adhesive peptides and calcium phosphate nanoparticles

T2 - In vitro evaluation of cell behavior

AU - Bongio, M.

AU - van den Beucken, J. J J P

AU - Nejadnik, M. R.

AU - Leeuwenburgh, S. C G

AU - Kinard, L. A.

AU - Kasper, F. K.

AU - Mikos, A. G.

AU - Jansen, J. A.

PY - 2011/12

Y1 - 2011/12

N2 - The ultimate goal of this work was to develop a biocompatible and biomimetic in situ crosslinkable hydrogel scaffold with an instructive capacity for bone regenerative treatment. To this end, synthetic hydrogels were functionalized with two key components of the extracellular matrix of native bone tissue, i.e. the three-amino acid peptide sequence RGD (which is the principal integrin-binding domain responsible for cell adhesion and survival of anchorage-dependent cells) and calcium phosphate (CaP) nanoparticles in the form of hydroxyapatite (which are similar to the inorganic phase of bone tissue). Rat bone marrow osteoblast-like cells (OBLCs) were encapsulated in four different biomaterials (plain oligo(poly(ethylene glycol) fumarate) (OPF), RGD-modified OPF, OPF enriched with CaP nanoparticles and RGD-modified OPF enriched with CaP nanoparticles) and cell survival, cell spreading, proliferation and mineralized matrix formation were determined via cell viability assay, histology and biochemical analysis for alkaline phosphatase activity and calcium. This study showed that RGD peptide sequences promoted cell spreading in OPF hydrogels and hence play a crucial role in cell survival during the early stage of culture, whereas CaP nanoparticles significantly enhanced cell-mediated hydrogel mineralization. Although cell spreading and proliferation activity were inhibited, the combined effect of RGD peptide sequences and CaP nanoparticles within OPF hydrogel systems elicited a better biological response than that of the individual components. Specifically, both a sustained cell viability and mineralized matrix production mediated by encapsulated OBLCs were observed within these novel biomimetic composite systems.

AB - The ultimate goal of this work was to develop a biocompatible and biomimetic in situ crosslinkable hydrogel scaffold with an instructive capacity for bone regenerative treatment. To this end, synthetic hydrogels were functionalized with two key components of the extracellular matrix of native bone tissue, i.e. the three-amino acid peptide sequence RGD (which is the principal integrin-binding domain responsible for cell adhesion and survival of anchorage-dependent cells) and calcium phosphate (CaP) nanoparticles in the form of hydroxyapatite (which are similar to the inorganic phase of bone tissue). Rat bone marrow osteoblast-like cells (OBLCs) were encapsulated in four different biomaterials (plain oligo(poly(ethylene glycol) fumarate) (OPF), RGD-modified OPF, OPF enriched with CaP nanoparticles and RGD-modified OPF enriched with CaP nanoparticles) and cell survival, cell spreading, proliferation and mineralized matrix formation were determined via cell viability assay, histology and biochemical analysis for alkaline phosphatase activity and calcium. This study showed that RGD peptide sequences promoted cell spreading in OPF hydrogels and hence play a crucial role in cell survival during the early stage of culture, whereas CaP nanoparticles significantly enhanced cell-mediated hydrogel mineralization. Although cell spreading and proliferation activity were inhibited, the combined effect of RGD peptide sequences and CaP nanoparticles within OPF hydrogel systems elicited a better biological response than that of the individual components. Specifically, both a sustained cell viability and mineralized matrix production mediated by encapsulated OBLCs were observed within these novel biomimetic composite systems.

KW - Biomimetic hydrogels

KW - Calcium phosphate nanoparticles

KW - Cell-material interactions

KW - In vitro mineralization

KW - Rgd peptide

KW - Synthetic polymers

UR - http://www.scopus.com/inward/record.url?scp=84857953503&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84857953503&partnerID=8YFLogxK

M3 - Article

C2 - 22179935

AN - SCOPUS:84857953503

VL - 22

SP - 359

EP - 376

JO - European Cells and Materials

JF - European Cells and Materials

SN - 1473-2262

ER -