Biomimetic mri contrast agent for imaging of inflammation in atherosclerotic plaque of apoe-/- mice

Hasan Alsaid, Genevieve De Souza, Marie Claude Bourdillon, Frédéric Chaubet, Abdulrazzaq Sulaiman, Catherine Desbleds-Mansard, Linda Chaabane, Charaf Zahir, Eric Lancelot, Olivier Rousseaux, Claire Corot, Philippe Douek, Andre Briguet, Didier Letourneur, Emmanuelle Canet-Soulas

Research output: Contribution to journalArticle

Abstract

Objective: Atherosclerosis involves an inflammatory process characterized by cellular and molecular responses. A slow-clearance blood-pool paramagnetic agent (CMD-A2-Gd-DOTA: P717) chemically modified to create a functionalized product (F-P717) for targeting inflammation in vessel walls was evaluated in vivo in mice. Methods and Results: Carboxylate and sulfate groups were grafted onto the macromolecular paramagnetic Gd-DOTA-dextran backbone. Products were also fluorescently labeled with rhodamine isothiocyanate. Pre- and postcontrast MRI was performed on a 2-Tesla magnet in ApoE-/- and control C57BL/6 mice after P717 or F-P717 injection at a dose of 60 μ mol Gd/kg. Axial T1-weighted images of the abdominal aorta were obtained using a 2D multislice spin-echo sequence. F-P717 significantly enhanced the magnetic resonance imaging (MRI) signal in the abdominal aortic wall of ApoE-/-mice (>50% signal-to-noise ratio increase between 10 and 30 minutes), but not of control mice. P717 produced only moderate (-/- mice colocalized F-P717 but not P717 with the inflammatory area revealed by P-selectin labeling. Conclusion: This study demonstrates the efficacy of F-P717 as a new molecular imaging agent for noninvasive in vivo MRI location of inflammatory vascular tree lesions in ApoE-/- mice.

Original languageEnglish
Pages (from-to)151-158
Number of pages8
JournalInvestigative Radiology
Volume44
Issue number3
DOIs
Publication statusPublished - Mar 2009

Keywords

  • Apolipoprotein E knockout mice
  • Atherosclerotic plaque
  • Biomimetic contrast agent
  • Inflammation
  • Magnetic resonance imaging

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging

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  • Cite this

    Alsaid, H., De Souza, G., Bourdillon, M. C., Chaubet, F., Sulaiman, A., Desbleds-Mansard, C., Chaabane, L., Zahir, C., Lancelot, E., Rousseaux, O., Corot, C., Douek, P., Briguet, A., Letourneur, D., & Canet-Soulas, E. (2009). Biomimetic mri contrast agent for imaging of inflammation in atherosclerotic plaque of apoe-/- mice. Investigative Radiology, 44(3), 151-158. https://doi.org/10.1097/RLI.0b013e31819472ac