Biomimetic PMMA-based bone substitutes: A comparative in vitro evaluation of the effects of pulsed electromagnetic field exposure

Paola Torricelli, M. Fini, G. Giavaresi, R. Botter, D. Beruto, R. Giardino

Research output: Contribution to journalArticle

Abstract

Pulsed electromagnetic fields (PEMFs) are known to be effective in the stimulation of cultured osteoblasts and in vivo healing of delayed and nonunion fractures. In the present in vitro study the effects of PEMFs on osteoblastic cell cultures (MG63 human osteoblast-like cells) grown in the presence of poly-methylmethacrylate (PMMA) and of a biomimetic bone substitute made of a PMMA matrix added with alfa-tricalcium phosphate (PMMA+α-TCP) were evaluated, to assess the biological response at the cell-biomaterial interaction. Cultures were stimulated with PEMFs (75 Hz, 2.3 mT, 1.3-ms pulse duration) 12 h/day for 3 days and evaluations (MTT, ALP, OC, PICP, TGFβ-1, IL-6) were performed at 3 and 6 days. PMMA had a negative effect on osteoblasts, whereas PMMA+α-TCP enhanced production of ALP, PICP, OC and TGFβ-1, and reduced IL-6 levels. Cells responded positively to PEMF stimulation even when cultured with a poorly biocompatible material, such as PMMA. This effect was more evident in the presence of PMMA+α-TCP (further improvement in proliferation and synthetic activity) both at 3 and at 6 days. The properties of PMMA+α-TCP look promising, and the present results support the use of PEMFs to improve tissue response to biomaterials implanted as bone substitutes.

Original languageEnglish
Pages (from-to)182-188
Number of pages7
JournalJournal of Biomedical Materials Research - Part A
Volume64
Issue number1
Publication statusPublished - Jan 1 2003

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Methylmethacrylate
Bone Substitutes
Biomimetics
Electromagnetic Fields
Electromagnetic fields
Bone
Osteoblasts
Biomaterials
Biocompatible Materials
Interleukin-6
Cell culture
Phosphates
In Vitro Techniques
Tissue
Cell Communication
Cell Culture Techniques

Keywords

  • Biomaterials
  • Bone substitutes
  • Osteoblasts
  • Pulsed electromagnetic field

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials

Cite this

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title = "Biomimetic PMMA-based bone substitutes: A comparative in vitro evaluation of the effects of pulsed electromagnetic field exposure",
abstract = "Pulsed electromagnetic fields (PEMFs) are known to be effective in the stimulation of cultured osteoblasts and in vivo healing of delayed and nonunion fractures. In the present in vitro study the effects of PEMFs on osteoblastic cell cultures (MG63 human osteoblast-like cells) grown in the presence of poly-methylmethacrylate (PMMA) and of a biomimetic bone substitute made of a PMMA matrix added with alfa-tricalcium phosphate (PMMA+α-TCP) were evaluated, to assess the biological response at the cell-biomaterial interaction. Cultures were stimulated with PEMFs (75 Hz, 2.3 mT, 1.3-ms pulse duration) 12 h/day for 3 days and evaluations (MTT, ALP, OC, PICP, TGFβ-1, IL-6) were performed at 3 and 6 days. PMMA had a negative effect on osteoblasts, whereas PMMA+α-TCP enhanced production of ALP, PICP, OC and TGFβ-1, and reduced IL-6 levels. Cells responded positively to PEMF stimulation even when cultured with a poorly biocompatible material, such as PMMA. This effect was more evident in the presence of PMMA+α-TCP (further improvement in proliferation and synthetic activity) both at 3 and at 6 days. The properties of PMMA+α-TCP look promising, and the present results support the use of PEMFs to improve tissue response to biomaterials implanted as bone substitutes.",
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AU - Torricelli, Paola

AU - Fini, M.

AU - Giavaresi, G.

AU - Botter, R.

AU - Beruto, D.

AU - Giardino, R.

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N2 - Pulsed electromagnetic fields (PEMFs) are known to be effective in the stimulation of cultured osteoblasts and in vivo healing of delayed and nonunion fractures. In the present in vitro study the effects of PEMFs on osteoblastic cell cultures (MG63 human osteoblast-like cells) grown in the presence of poly-methylmethacrylate (PMMA) and of a biomimetic bone substitute made of a PMMA matrix added with alfa-tricalcium phosphate (PMMA+α-TCP) were evaluated, to assess the biological response at the cell-biomaterial interaction. Cultures were stimulated with PEMFs (75 Hz, 2.3 mT, 1.3-ms pulse duration) 12 h/day for 3 days and evaluations (MTT, ALP, OC, PICP, TGFβ-1, IL-6) were performed at 3 and 6 days. PMMA had a negative effect on osteoblasts, whereas PMMA+α-TCP enhanced production of ALP, PICP, OC and TGFβ-1, and reduced IL-6 levels. Cells responded positively to PEMF stimulation even when cultured with a poorly biocompatible material, such as PMMA. This effect was more evident in the presence of PMMA+α-TCP (further improvement in proliferation and synthetic activity) both at 3 and at 6 days. The properties of PMMA+α-TCP look promising, and the present results support the use of PEMFs to improve tissue response to biomaterials implanted as bone substitutes.

AB - Pulsed electromagnetic fields (PEMFs) are known to be effective in the stimulation of cultured osteoblasts and in vivo healing of delayed and nonunion fractures. In the present in vitro study the effects of PEMFs on osteoblastic cell cultures (MG63 human osteoblast-like cells) grown in the presence of poly-methylmethacrylate (PMMA) and of a biomimetic bone substitute made of a PMMA matrix added with alfa-tricalcium phosphate (PMMA+α-TCP) were evaluated, to assess the biological response at the cell-biomaterial interaction. Cultures were stimulated with PEMFs (75 Hz, 2.3 mT, 1.3-ms pulse duration) 12 h/day for 3 days and evaluations (MTT, ALP, OC, PICP, TGFβ-1, IL-6) were performed at 3 and 6 days. PMMA had a negative effect on osteoblasts, whereas PMMA+α-TCP enhanced production of ALP, PICP, OC and TGFβ-1, and reduced IL-6 levels. Cells responded positively to PEMF stimulation even when cultured with a poorly biocompatible material, such as PMMA. This effect was more evident in the presence of PMMA+α-TCP (further improvement in proliferation and synthetic activity) both at 3 and at 6 days. The properties of PMMA+α-TCP look promising, and the present results support the use of PEMFs to improve tissue response to biomaterials implanted as bone substitutes.

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