Biomonitoring of exposure to urban air pollutants

analysis of sister chromatid exchanges and DNA lesions in peripheral lymphocytes of traffic policemen.

A. Carere, C. Andreoli, R. Galati, P. Leopardi, F. Marcon, M. V. Rosati, S. Rossi, F. Tomei, A. Verdina, A. Zijno, R. Crebelli

Research output: Contribution to journalArticle

Abstract

In order to elucidate the health effects of occupational exposure to traffic fumes, a few biomarkers of early genetic effect were investigated in Rome traffic policemen. One hundred and ninety healthy subjects engaged in traffic control (133 subjects) or in office work (57 subjects) participated the study. For all subjects, detailed information on smoking habits and other potential confounders were recorded by questionnaires. Average exposure of the study groups to benzene and other aromatic hydrocarbons was evaluated in a parallel exposure survey. All workers were genotyped for the following metabolic polymorphisms: CYP1A1 (m1, m2, and m4 variants), CYP2E1 (PstI and RsaI), NQO1 (Hinf1), GSTM1 and GSTT1 (null variants). In this paper, the results of the analysis of sister chromatid exchanges (SCE) in peripheral lymphocytes, and DNA damage by alkaline (pH 13) comet assay in mononuclear blood cells are reported. No statistically significant difference in the frequency of SCE or high frequency cells (HFC) was observed between traffic wardens and office workers (controls), despite the significantly higher exposure to benzene of the former (average group exposure 9.5 versus 3.8microg/m(3), 7h TWA). Conversely, both SCE per cell and HFC were highly significantly (P

Original languageEnglish
Pages (from-to)215-224
Number of pages10
JournalMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis
Volume518
Issue number2
Publication statusPublished - Jul 25 2002

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Air Pollutants
Sister Chromatid Exchange
Environmental Monitoring
Lymphocytes
Benzene
DNA
Aromatic Hydrocarbons
Cytochrome P-450 CYP2E1
Cytochrome P-450 CYP1A1
Comet Assay
Occupational Exposure
DNA Damage
Habits
Blood Cells
Healthy Volunteers
Biomarkers
Smoking
Health
Surveys and Questionnaires

ASJC Scopus subject areas

  • Health, Toxicology and Mutagenesis
  • Molecular Biology

Cite this

Biomonitoring of exposure to urban air pollutants : analysis of sister chromatid exchanges and DNA lesions in peripheral lymphocytes of traffic policemen. / Carere, A.; Andreoli, C.; Galati, R.; Leopardi, P.; Marcon, F.; Rosati, M. V.; Rossi, S.; Tomei, F.; Verdina, A.; Zijno, A.; Crebelli, R.

In: Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, Vol. 518, No. 2, 25.07.2002, p. 215-224.

Research output: Contribution to journalArticle

Carere, A. ; Andreoli, C. ; Galati, R. ; Leopardi, P. ; Marcon, F. ; Rosati, M. V. ; Rossi, S. ; Tomei, F. ; Verdina, A. ; Zijno, A. ; Crebelli, R. / Biomonitoring of exposure to urban air pollutants : analysis of sister chromatid exchanges and DNA lesions in peripheral lymphocytes of traffic policemen. In: Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis. 2002 ; Vol. 518, No. 2. pp. 215-224.
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