Abstract
In order to elucidate the health effects of occupational exposure to traffic fumes, a few biomarkers of early genetic effect were investigated in Rome traffic policemen. One hundred and ninety healthy subjects engaged in traffic control (133 subjects) or in office work (57 subjects) participated the study. For all subjects, detailed information on smoking habits and other potential confounders were recorded by questionnaires. Average exposure of the study groups to benzene and other aromatic hydrocarbons was evaluated in a parallel exposure survey. All workers were genotyped for the following metabolic polymorphisms: CYP1A1 (m1, m2, and m4 variants), CYP2E1 (PstI and RsaI), NQO1 (Hinf1), GSTM1 and GSTT1 (null variants). In this paper, the results of the analysis of sister chromatid exchanges (SCE) in peripheral lymphocytes, and DNA damage by alkaline (pH 13) comet assay in mononuclear blood cells are reported. No statistically significant difference in the frequency of SCE or high frequency cells (HFC) was observed between traffic wardens and office workers (controls), despite the significantly higher exposure to benzene of the former (average group exposure 9.5 versus 3.8microg/m(3), 7h TWA). Conversely, both SCE per cell and HFC were highly significantly (P
| Original language | English |
|---|---|
| Pages (from-to) | 215-224 |
| Number of pages | 10 |
| Journal | Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis |
| Volume | 518 |
| Issue number | 2 |
| Publication status | Published - Jul 25 2002 |
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ASJC Scopus subject areas
- Health, Toxicology and Mutagenesis
- Molecular Biology
Cite this
Biomonitoring of exposure to urban air pollutants : analysis of sister chromatid exchanges and DNA lesions in peripheral lymphocytes of traffic policemen. / Carere, A.; Andreoli, C.; Galati, R.; Leopardi, P.; Marcon, F.; Rosati, M. V.; Rossi, S.; Tomei, F.; Verdina, A.; Zijno, A.; Crebelli, R.
In: Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, Vol. 518, No. 2, 25.07.2002, p. 215-224.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Biomonitoring of exposure to urban air pollutants
T2 - analysis of sister chromatid exchanges and DNA lesions in peripheral lymphocytes of traffic policemen.
AU - Carere, A.
AU - Andreoli, C.
AU - Galati, R.
AU - Leopardi, P.
AU - Marcon, F.
AU - Rosati, M. V.
AU - Rossi, S.
AU - Tomei, F.
AU - Verdina, A.
AU - Zijno, A.
AU - Crebelli, R.
PY - 2002/7/25
Y1 - 2002/7/25
N2 - In order to elucidate the health effects of occupational exposure to traffic fumes, a few biomarkers of early genetic effect were investigated in Rome traffic policemen. One hundred and ninety healthy subjects engaged in traffic control (133 subjects) or in office work (57 subjects) participated the study. For all subjects, detailed information on smoking habits and other potential confounders were recorded by questionnaires. Average exposure of the study groups to benzene and other aromatic hydrocarbons was evaluated in a parallel exposure survey. All workers were genotyped for the following metabolic polymorphisms: CYP1A1 (m1, m2, and m4 variants), CYP2E1 (PstI and RsaI), NQO1 (Hinf1), GSTM1 and GSTT1 (null variants). In this paper, the results of the analysis of sister chromatid exchanges (SCE) in peripheral lymphocytes, and DNA damage by alkaline (pH 13) comet assay in mononuclear blood cells are reported. No statistically significant difference in the frequency of SCE or high frequency cells (HFC) was observed between traffic wardens and office workers (controls), despite the significantly higher exposure to benzene of the former (average group exposure 9.5 versus 3.8microg/m(3), 7h TWA). Conversely, both SCE per cell and HFC were highly significantly (P
AB - In order to elucidate the health effects of occupational exposure to traffic fumes, a few biomarkers of early genetic effect were investigated in Rome traffic policemen. One hundred and ninety healthy subjects engaged in traffic control (133 subjects) or in office work (57 subjects) participated the study. For all subjects, detailed information on smoking habits and other potential confounders were recorded by questionnaires. Average exposure of the study groups to benzene and other aromatic hydrocarbons was evaluated in a parallel exposure survey. All workers were genotyped for the following metabolic polymorphisms: CYP1A1 (m1, m2, and m4 variants), CYP2E1 (PstI and RsaI), NQO1 (Hinf1), GSTM1 and GSTT1 (null variants). In this paper, the results of the analysis of sister chromatid exchanges (SCE) in peripheral lymphocytes, and DNA damage by alkaline (pH 13) comet assay in mononuclear blood cells are reported. No statistically significant difference in the frequency of SCE or high frequency cells (HFC) was observed between traffic wardens and office workers (controls), despite the significantly higher exposure to benzene of the former (average group exposure 9.5 versus 3.8microg/m(3), 7h TWA). Conversely, both SCE per cell and HFC were highly significantly (P
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M3 - Article
VL - 518
SP - 215
EP - 224
JO - Mutation Research
JF - Mutation Research
SN - 0027-5107
IS - 2
ER -