bioNMR-based identification of natural anti-Aβ compounds in Peucedanum ostruthium

Alessandro Palmioli, Sara Bertuzzi, Ada De Luigi, Laura Colombo, Barbara La Ferla, Mario Salmona, Ivano De Noni, Cristina Airoldi

Research output: Contribution to journalArticle

Abstract

The growing interest in medicinal plants for the identification of new bioactive compounds and the formulation of new nutraceuticals and drugs prompted us to develop a powerful experimental approach allowing the detailed metabolic profiling of complex plant extracts, the identification of ligands of macromolecular targets of biomedical relevance and a preliminary characterization of their biological activity. To this end, we selected Peucedanum ostruthium, a plant traditionally employed in Austria and Italy for its several potential therapeutic applications, as case study. We combined the use of NMR and UPLC-HR-MS for the identification of the metabolites present in its leaves and rhizome extracts. Due to the significant content of polyphenols, particularly chlorogenic acids, recently identified as anti-amyloidogenic compounds, polyphenols-enriched fractions were prepared and tested for their ability to prevent Aβ1-42 peptide aggregation and neurotoxicity in a neuronal human cell line. STD-NMR experiments allowed the detailed identification of Aβ oligomers' ligands responsible for the anti-amyloidogenic activity. These data provide experimental protocols and structural information suitable for the development of innovative molecular tools for prevention, therapy and diagnosis of Alzheimer's disease.

Original languageEnglish
Pages (from-to)76-86
Number of pages11
JournalBioorganic Chemistry
Volume83
DOIs
Publication statusPublished - Mar 2019

Keywords

  • Amyloid beta-Peptides/antagonists & inhibitors
  • Apiaceae/chemistry
  • Biological Products/analysis
  • Dose-Response Relationship, Drug
  • Molecular Structure
  • Nuclear Magnetic Resonance, Biomolecular
  • Plant Extracts/analysis
  • Plant Leaves/chemistry
  • Structure-Activity Relationship

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