Biopolymeric modification of superoxide dismutase (mPEG-SOD) to prevent muscular ischemia-reperfusion damage

R. Giardino, S. Capelli, M. Fini, G. Giavaresi, L. Orienti, F. M. Veronese, P. Caliceti, M. Rocca

Research output: Contribution to journalArticle

Abstract

The efficacy of preventing ischemia-reperfusion damage by employing native or modified (mPEG-SOD) superoxide dismutase in an experimental animal model of acute ischemia of the left hindlimb was tested. Four hours and thirty minutes complete warm ischemia was induced in the left hindlimb of 43 Wistar rats by clamping the femoral artery and monitoring its efficacy with Laser Doppler flowmetry. After ten days, a significative difference (p = 0.004) of the survival leg rate was found in the group treated with mPEG-SOD (86.6%) compared with the control group (30%). Histomorphological and ultrastructural analysis were performed at different time intervals confirming what the clinical course had already pointed our. These results show that SOD in ifs modified form, despite the lower dosage, can provide good protection against ischemia/reperfusion injury of muscles.

Original languageEnglish
Pages (from-to)167-172
Number of pages6
JournalInternational Journal of Artificial Organs
Volume18
Issue number3
Publication statusPublished - 1995

Keywords

  • Experimental model
  • Limb ischemia
  • Modified superoxide dismutase
  • Reperfusion
  • Skeletal muscle

ASJC Scopus subject areas

  • Biophysics

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