Bioprinted 3D vascularized tissue model for drug toxicity analysis

Solange Massa, Mahmoud Ahmed Sakr, Jungmok Seo, Praveen Bandaru, Andrea Arneri, Simone Bersini, Elaheh Zare-Eelanjegh, Elmira Jalilian, Byung Hyun Cha, Silvia Antona, Alessandro Enrico, Yuan Gao, Shabir Hassan, Juan Pablo Acevedo, Mehmet R. Dokmeci, Yu Shrike Zhang, Ali Khademhosseini, Su Ryon Shin

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

To develop biomimetic three-dimensional (3D) tissue constructs for drug screening and biological studies, engineered blood vessels should be integrated into the constructs to mimic the drug administration process in vivo. The development of perfusable vascularized 3D tissue constructs for studying the drug administration process through an engineered endothelial layer remains an area of intensive research. Here, we report the development of a simple 3D vascularized liver tissue model to study drug toxicity through the incorporation of an engineered endothelial layer. Using a sacrificial bioprinting technique, a hollow microchannel was successfully fabricated in the 3D liver tissue construct created with HepG2/C3A cells encapsulated in a gelatin methacryloyl hydrogel. After seeding human umbilical vein endothelial cells (HUVECs) into the microchannel, we obtained a vascularized tissue construct containing a uniformly coated HUVEC layer within the hollow microchannel. The inclusion of the HUVEC layer into the scaffold resulted in delayed permeability of biomolecules into the 3D liver construct. In addition, the vascularized construct containing the HUVEC layer showed an increased viability of the HepG2/C3A cells within the 3D scaffold compared to that of the 3D liver constructs without the HUVEC layer, demonstrating a protective role of the introduced endothelial cell layer. The 3D vascularized liver model presented in this study is anticipated to provide a better and more accurate in vitro liver model system for future drug toxicity testing.

Original languageEnglish
Article number044109
JournalBiomicrofluidics
Volume11
Issue number4
DOIs
Publication statusPublished - Jul 1 2017

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Endothelial cells
Drug-Related Side Effects and Adverse Reactions
toxicity
Liver
Human Umbilical Vein Endothelial Cells
Toxicity
liver
drugs
veins
Tissue
Microchannels
Pharmaceutical Preparations
microchannels
Hep G2 Cells
Scaffolds (biology)
Bioprinting
hollow
Biomimetics
Preclinical Drug Evaluations
Hydrogel

ASJC Scopus subject areas

  • Molecular Biology
  • Materials Science(all)
  • Genetics
  • Condensed Matter Physics
  • Physical and Theoretical Chemistry

Cite this

Massa, S., Sakr, M. A., Seo, J., Bandaru, P., Arneri, A., Bersini, S., ... Shin, S. R. (2017). Bioprinted 3D vascularized tissue model for drug toxicity analysis. Biomicrofluidics, 11(4), [044109]. https://doi.org/10.1063/1.4994708

Bioprinted 3D vascularized tissue model for drug toxicity analysis. / Massa, Solange; Sakr, Mahmoud Ahmed; Seo, Jungmok; Bandaru, Praveen; Arneri, Andrea; Bersini, Simone; Zare-Eelanjegh, Elaheh; Jalilian, Elmira; Cha, Byung Hyun; Antona, Silvia; Enrico, Alessandro; Gao, Yuan; Hassan, Shabir; Acevedo, Juan Pablo; Dokmeci, Mehmet R.; Zhang, Yu Shrike; Khademhosseini, Ali; Shin, Su Ryon.

In: Biomicrofluidics, Vol. 11, No. 4, 044109, 01.07.2017.

Research output: Contribution to journalArticle

Massa, S, Sakr, MA, Seo, J, Bandaru, P, Arneri, A, Bersini, S, Zare-Eelanjegh, E, Jalilian, E, Cha, BH, Antona, S, Enrico, A, Gao, Y, Hassan, S, Acevedo, JP, Dokmeci, MR, Zhang, YS, Khademhosseini, A & Shin, SR 2017, 'Bioprinted 3D vascularized tissue model for drug toxicity analysis', Biomicrofluidics, vol. 11, no. 4, 044109. https://doi.org/10.1063/1.4994708
Massa S, Sakr MA, Seo J, Bandaru P, Arneri A, Bersini S et al. Bioprinted 3D vascularized tissue model for drug toxicity analysis. Biomicrofluidics. 2017 Jul 1;11(4). 044109. https://doi.org/10.1063/1.4994708
Massa, Solange ; Sakr, Mahmoud Ahmed ; Seo, Jungmok ; Bandaru, Praveen ; Arneri, Andrea ; Bersini, Simone ; Zare-Eelanjegh, Elaheh ; Jalilian, Elmira ; Cha, Byung Hyun ; Antona, Silvia ; Enrico, Alessandro ; Gao, Yuan ; Hassan, Shabir ; Acevedo, Juan Pablo ; Dokmeci, Mehmet R. ; Zhang, Yu Shrike ; Khademhosseini, Ali ; Shin, Su Ryon. / Bioprinted 3D vascularized tissue model for drug toxicity analysis. In: Biomicrofluidics. 2017 ; Vol. 11, No. 4.
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