Biopsy diagnosis of Creutzfeldt-Jakob disease by western blot: A case report

R. J. Castellani, P. Parchi, L. Madoff, P. Gambetti, P. Mckeever

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Creutzfeldt-Jakob disease is a clinically and pathologically heterogeneous disorder that often requires brain biopsy for definitive diagnosis. We report the case of a 62-year-old man who underwent brain biopsy for progressive neurological deterioration. Histopathologically, there was minimal spongiform change that could not be unequivocally attributed to Creutzfeldt-Jakob disease. A 16 mg portion of gray matter saved frozen was subsequently analyzed by Western blot and showed definitive protease- resistant prion protein. This case illustrates applicability, ease in interpretation, and accuracy of Western blot analysis for protease-resistant prion protein in small brain biopsy specimens. Given the importance of accurate diagnosis in suspected prion disease, we recommend that a small portion of tissue from any brain biopsy performed in this setting be kept frozen for possible biochemical studies.

Original languageEnglish
Pages (from-to)623-625
Number of pages3
JournalHuman Pathology
Volume28
Issue number5
DOIs
Publication statusPublished - 1997

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Creutzfeldt-Jakob Syndrome
Western Blotting
Biopsy
Brain
Peptide Hydrolases
Prion Diseases
Prion Proteins

Keywords

  • Creutzfeldt-Jakob disease
  • electron microscopy
  • immunohistochemistry
  • prion protein
  • Western blot

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Biopsy diagnosis of Creutzfeldt-Jakob disease by western blot : A case report. / Castellani, R. J.; Parchi, P.; Madoff, L.; Gambetti, P.; Mckeever, P.

In: Human Pathology, Vol. 28, No. 5, 1997, p. 623-625.

Research output: Contribution to journalArticle

Castellani, R. J. ; Parchi, P. ; Madoff, L. ; Gambetti, P. ; Mckeever, P. / Biopsy diagnosis of Creutzfeldt-Jakob disease by western blot : A case report. In: Human Pathology. 1997 ; Vol. 28, No. 5. pp. 623-625.
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