Biosynthesis and apical localization of the urokinase receptor in polarized MDCK epithelial cells

Paola Limongi, Massimo Resnati, Luciano Hernandez-Marrero, Ottavio Cremona, Francesco Blasi, Francesca Fazioli

Research output: Contribution to journalArticlepeer-review

Abstract

The biosynthesis and the surface localization of the urokinase plasminogen activator receptor (uPAR) were analysed in MDCK epithelial cells and in unpolarized fibroblasts. No differences were observed with respect to rate of synthesis, nature of precursors and time of surface appearance. uPAR was localized particularly at the focal and cell-cell contacts when expressed in fibroblasts. On the contrary, in MDCK cells uPAR was found mostly on the apical surface; in agreement with its localization, down-regulation of uPAR by the uPA-PAI-1 complex was observed only from the apical membrane.

Original languageEnglish
Pages (from-to)207-211
Number of pages5
JournalFEBS Letters
Volume369
Issue number2-3
DOIs
Publication statusPublished - Aug 7 1995

Keywords

  • GPI-anchored protein
  • MDCK cell
  • Urokinase receptor

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Genetics
  • Molecular Biology
  • Structural Biology

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