TY - JOUR
T1 - Biosynthesis of dTTP during differentation of chick embryo myoblasts in culture
T2 - Changes in thymidine kinase, thymidilate kinase and thymidilate synthetase activities
AU - Cossu, Guilio
AU - Grippo, Pasquale
AU - Marino, Mirella
AU - Molinaro, Mario
PY - 1979
Y1 - 1979
N2 - Thymidine kinase, thymidilate kinase and thymidilate synthetase activities have been studied during differentation in culture of muscle cells from chick embryo both in dplicating myoblasts and in post-mitotic myotubes. Thymidine kinase activity starts decreasing long before fusion, when myoblasts are still actively engaged in DNA synthesis and it reaches a minimum value at fusion (approx. 70 h of culture). Also thymidilate synthetase undergoes a precocious alteration of its activity which is low at 24 h culture, it increases up to 48 h and drops to a very low level after fusion. Finally, thymidilate kinase activity remains constant during the duplicative period and declines only after fusion. Pulse-labelling experiments with [3H]thymidine and [3H]deoxycytidine show that the incorporation of these precursors into the deoxynucleotide pool and into DNA parallels the patterns of the enzymatic activities measured in vitro.
AB - Thymidine kinase, thymidilate kinase and thymidilate synthetase activities have been studied during differentation in culture of muscle cells from chick embryo both in dplicating myoblasts and in post-mitotic myotubes. Thymidine kinase activity starts decreasing long before fusion, when myoblasts are still actively engaged in DNA synthesis and it reaches a minimum value at fusion (approx. 70 h of culture). Also thymidilate synthetase undergoes a precocious alteration of its activity which is low at 24 h culture, it increases up to 48 h and drops to a very low level after fusion. Finally, thymidilate kinase activity remains constant during the duplicative period and declines only after fusion. Pulse-labelling experiments with [3H]thymidine and [3H]deoxycytidine show that the incorporation of these precursors into the deoxynucleotide pool and into DNA parallels the patterns of the enzymatic activities measured in vitro.
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U2 - 10.1016/0045-6039(79)90007-1
DO - 10.1016/0045-6039(79)90007-1
M3 - Article
C2 - 228863
AN - SCOPUS:0018696314
VL - 8
SP - 323
EP - 329
JO - Cell Differentiation
JF - Cell Differentiation
SN - 0045-6039
IS - 4
ER -