Biosynthesis of Hb in individual fetal liver bursts. γ-Chain production peaks earlier than β-chain in the erythropoietic pathway

A. M. Gianni, P. Comi, B. Giglioni, S. Ottolenghi, A. R. Migliaccio, G. Migliaccio, F. Lettieri, Y. P. Maguire, C. Peschle

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Abstract

The relative synthesis of α-, β-, Gγ- and Aγ-globin chains has been evaluated in single fetal liver bursts, which were grown in methylcellulose cultures, individually labelled with [3H]leucine and then analysed via iso-electric focusing. Well-hemoglobinized bursts demonstrate a homogeneous globin synthetic pattern, characterized by prevalent HbF (+some HbA) synthesis: thus, they apparently originate from a homogeneously programmed population of erythroid burst-forming unit (BFU-E). On day 8-9 of culture, the synthetic pattern in 'mature' (i.e., well-hemoglobinized) bursts has been compared with that in simultaneously-grown, 'immature' (i.e., poorly-hemoglobinized) colonies. These patterns have been further compared with that in 'matured' bursts (identified in situ as immature on day 8-9 and labelled 2-4 days later when matured). The 'immature' colonies showed very low levels of relative β-globin synthesis, while the 'mature' ones demonstrated a more elevated production of β-chain. Significantly, the 'matured' bursts showed a globin chain synthetic pattern similar to that of previously labelled 'matured' colonies. It is postulated therefore that in fetal liver (and also in adult marrow) the synthesis of γ-chain is linked to an early differentiation stage of erythroblasts, while β-globin synthesis is largely activated at a more advanced maturation stage.

Original languageEnglish
Pages (from-to)345-352
Number of pages8
JournalExperimental Cell Research
Volume130
Issue number2
DOIs
Publication statusPublished - 1980

ASJC Scopus subject areas

  • Cell Biology

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    Gianni, A. M., Comi, P., Giglioni, B., Ottolenghi, S., Migliaccio, A. R., Migliaccio, G., Lettieri, F., Maguire, Y. P., & Peschle, C. (1980). Biosynthesis of Hb in individual fetal liver bursts. γ-Chain production peaks earlier than β-chain in the erythropoietic pathway. Experimental Cell Research, 130(2), 345-352. https://doi.org/10.1016/0014-4827(80)90011-7