BisEDT and RIP act in synergy to prevent graft infections by resistant staphylococci

Philip Domenico, Ellen Gurzenda, Andrea Giacometti, Oscar Cirioni, Roberto Ghiselli, Fiorenza Orlando, Moshe Korem, Vittorio Saba, Giorgio Scalise, Naomi Balaban

Research output: Contribution to journalArticle

Abstract

Staphylococci are a major cause of infections associated with indwelling medical devices. Biofilm formation on these devices adds to the antibiotic resistance seen among clinical isolates. RNAIII-inhibiting peptide (RIP) is a heptapeptide that inhibits staphylococcal pathogenesis, including biofilm formation, by obstructing quorum sensing mechanisms. Bismuth ethanedithiol (BisEDT) also prevents biofilm formation at subinhibitory concentrations. RIP and BisEDT were combined to prevent infections in a rat graft model, using antibiotic sensitive and resistant strains of Staphylococcus aureus and Staphylococcus epidermidis. BisEDT, RIP, or rifampin, or their combinations reduced the graft associated bacterial load over seven days. BisEDT-RIP was the best combination, reducing bacterial load to undetectable levels. BisEDT-RIP may prove useful for coating medical devices to prevent staphylococcal infections.

Original languageEnglish
Pages (from-to)2047-2053
Number of pages7
JournalPeptides
Volume25
Issue number12
DOIs
Publication statusPublished - Dec 2004

Keywords

  • Antimicrobial peptides
  • Biofilms
  • Bismuth thiols
  • RNAIII inhibiting peptide
  • Staphylococci

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Physiology
  • Cellular and Molecular Neuroscience

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  • Cite this

    Domenico, P., Gurzenda, E., Giacometti, A., Cirioni, O., Ghiselli, R., Orlando, F., Korem, M., Saba, V., Scalise, G., & Balaban, N. (2004). BisEDT and RIP act in synergy to prevent graft infections by resistant staphylococci. Peptides, 25(12), 2047-2053. https://doi.org/10.1016/j.peptides.2004.08.005