BKV infection and hemorrhagic cystitis after allogeneic bone marrow transplant

D. Fioriti, A. M. Degener, M. Mischitelli, M. Videtta, A. Arancio, S. Sica, F. Sorà, Valeria Pietropaolo

Research output: Contribution to journalArticle

Abstract

Hemorrhagic cystitis (HC) is a well-known complication after allogeneic bone marrow transplant (BMT) and can be related to adenovirus or human polyomavirus BK (BKV) infections. In this study a group of 20 patients after allogeneic BMT has been examined. BMT urine samples were analysed for the presence of Adenovirus and BKV DNA by means of polymerase chain reaction (PCR). 5/20 BMT patients developed HC after BMT. The presence of BKV DNA in urine samples was evident in 3/15 patients without HC and in 5/5 patients with HC. In 2/5 HC-patients the BKV DNA was not found after therapy with Cidofovir and Ribavirin. The search for adenovirus DNA in all samples was negative. The analysis of BKV non-coding control region (NCCR) isolated from urine samples revealed a structure very similar to the archetype in all samples. The RFLP (Restriction Fragment Length Polymorphism assay) showed the presence of BKV subtypes I and IV, with the prevalence of subtype I (4/5). This study supports the hypothesis that HC is mainly related to BKV rather than to adenovirus infection in BMT patients. Moreover, since BKV subtype I was predominant, it is reasonable to hypothesize that a specific BKV subtype could be associated with the development of HC.

Original languageEnglish
Pages (from-to)309-316
Number of pages8
JournalInternational Journal of Immunopathology and Pharmacology
Volume18
Issue number2
Publication statusPublished - Apr 2005

Fingerprint

Cystitis
Bone Marrow
Transplants
Infection
Adenoviridae
Urine
DNA
BK Virus
Adenoviridae Infections
Ribavirin
DNA-Directed DNA Polymerase
Restriction Fragment Length Polymorphisms
Polymerase Chain Reaction

Keywords

  • Adenovirus
  • BKV
  • Hemorrhagic cystitis
  • Polymerase chain reaction

ASJC Scopus subject areas

  • Pharmacology

Cite this

Fioriti, D., Degener, A. M., Mischitelli, M., Videtta, M., Arancio, A., Sica, S., ... Pietropaolo, V. (2005). BKV infection and hemorrhagic cystitis after allogeneic bone marrow transplant. International Journal of Immunopathology and Pharmacology, 18(2), 309-316.

BKV infection and hemorrhagic cystitis after allogeneic bone marrow transplant. / Fioriti, D.; Degener, A. M.; Mischitelli, M.; Videtta, M.; Arancio, A.; Sica, S.; Sorà, F.; Pietropaolo, Valeria.

In: International Journal of Immunopathology and Pharmacology, Vol. 18, No. 2, 04.2005, p. 309-316.

Research output: Contribution to journalArticle

Fioriti, D, Degener, AM, Mischitelli, M, Videtta, M, Arancio, A, Sica, S, Sorà, F & Pietropaolo, V 2005, 'BKV infection and hemorrhagic cystitis after allogeneic bone marrow transplant', International Journal of Immunopathology and Pharmacology, vol. 18, no. 2, pp. 309-316.
Fioriti D, Degener AM, Mischitelli M, Videtta M, Arancio A, Sica S et al. BKV infection and hemorrhagic cystitis after allogeneic bone marrow transplant. International Journal of Immunopathology and Pharmacology. 2005 Apr;18(2):309-316.
Fioriti, D. ; Degener, A. M. ; Mischitelli, M. ; Videtta, M. ; Arancio, A. ; Sica, S. ; Sorà, F. ; Pietropaolo, Valeria. / BKV infection and hemorrhagic cystitis after allogeneic bone marrow transplant. In: International Journal of Immunopathology and Pharmacology. 2005 ; Vol. 18, No. 2. pp. 309-316.
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