Blastocystis hominis transmission by non-potable water: a case report in Italy

Maria Cristina Angelici, Chiara Nardis, Riccardo Scarpelli, Paola Ade

Research output: Contribution to journalArticle

Abstract

In the reported case, a 41-year-old Italian man came to the clinician's observation reporting cramps, bloating and watery diarrhoea a few days after drinking water indicated as unpotable from a fountain in a farm area. The medical suspicion was directed at both gluten intolerance and enteric infection, eventually of waterborne origin. Gluten intolerance was investigated by intestinal biopsy and excluded, while stool analyses ruled out infective bacteriological or viral agents and parasites. Subsequently, a persistent eosinophilia was revealed and a parasitological analysis was again suggested, planning for a more sensitive molecular method. Therefore, a multiplex-PCR of enteric protozoa species DNA was performed on an intestinal biopsy and faecal samples revealing only Blastocystis hominis protozoa, subsequently typed as subtype 1 by RFLP-PCR method. B. hominis is an anaerobic protozoa found in the human and animal intestinal tract, recently associated with a pathogenic role characterized by chronic development. Since blastocystosis has been demonstrated as a waterborne infection, a sample of water matrix was analysed, revealing the B. hominis subtype 1 DNA inside. A probable water transmission of Blastocystis infection has been demonstrated in this case report. Only a probiotic treatment based on Saccharomyces boulardii was administered to the patient and this apparently resolved the infection. In summary, the case described here is a chronic blastocystosis of possible waterborne origin, controlled by assuming a yeast treatment.

Original languageEnglish
Pages (from-to)173-177
Number of pages5
JournalThe new microbiologica
Volume41
Issue number2
Publication statusPublished - Apr 2018

Fingerprint

Blastocystis hominis
Italy
Glutens
Blastocystis Infections
Water
Infection
Biopsy
Muscle Cramp
Multiplex Polymerase Chain Reaction
DNA
Probiotics
Eosinophilia
Drinking Water
Restriction Fragment Length Polymorphisms
Diarrhea
Parasites
Yeasts
Observation
Polymerase Chain Reaction
Therapeutics

Keywords

  • Adult
  • Blastocystis Infections/parasitology
  • Blastocystis hominis/isolation & purification
  • Humans
  • Italy
  • Male
  • Probiotics/therapeutic use
  • Water/parasitology

Cite this

Blastocystis hominis transmission by non-potable water : a case report in Italy. / Angelici, Maria Cristina; Nardis, Chiara; Scarpelli, Riccardo; Ade, Paola.

In: The new microbiologica, Vol. 41, No. 2, 04.2018, p. 173-177.

Research output: Contribution to journalArticle

Angelici, MC, Nardis, C, Scarpelli, R & Ade, P 2018, 'Blastocystis hominis transmission by non-potable water: a case report in Italy', The new microbiologica, vol. 41, no. 2, pp. 173-177.
Angelici, Maria Cristina ; Nardis, Chiara ; Scarpelli, Riccardo ; Ade, Paola. / Blastocystis hominis transmission by non-potable water : a case report in Italy. In: The new microbiologica. 2018 ; Vol. 41, No. 2. pp. 173-177.
@article{6c5279c8c88d45fda9c13efad0ae5363,
title = "Blastocystis hominis transmission by non-potable water: a case report in Italy",
abstract = "In the reported case, a 41-year-old Italian man came to the clinician's observation reporting cramps, bloating and watery diarrhoea a few days after drinking water indicated as unpotable from a fountain in a farm area. The medical suspicion was directed at both gluten intolerance and enteric infection, eventually of waterborne origin. Gluten intolerance was investigated by intestinal biopsy and excluded, while stool analyses ruled out infective bacteriological or viral agents and parasites. Subsequently, a persistent eosinophilia was revealed and a parasitological analysis was again suggested, planning for a more sensitive molecular method. Therefore, a multiplex-PCR of enteric protozoa species DNA was performed on an intestinal biopsy and faecal samples revealing only Blastocystis hominis protozoa, subsequently typed as subtype 1 by RFLP-PCR method. B. hominis is an anaerobic protozoa found in the human and animal intestinal tract, recently associated with a pathogenic role characterized by chronic development. Since blastocystosis has been demonstrated as a waterborne infection, a sample of water matrix was analysed, revealing the B. hominis subtype 1 DNA inside. A probable water transmission of Blastocystis infection has been demonstrated in this case report. Only a probiotic treatment based on Saccharomyces boulardii was administered to the patient and this apparently resolved the infection. In summary, the case described here is a chronic blastocystosis of possible waterborne origin, controlled by assuming a yeast treatment.",
keywords = "Adult, Blastocystis Infections/parasitology, Blastocystis hominis/isolation & purification, Humans, Italy, Male, Probiotics/therapeutic use, Water/parasitology",
author = "Angelici, {Maria Cristina} and Chiara Nardis and Riccardo Scarpelli and Paola Ade",
year = "2018",
month = "4",
language = "English",
volume = "41",
pages = "173--177",
journal = "New Microbiologica",
issn = "1121-7138",
publisher = "Luigi Ponzio e figlio Editori",
number = "2",

}

TY - JOUR

T1 - Blastocystis hominis transmission by non-potable water

T2 - a case report in Italy

AU - Angelici, Maria Cristina

AU - Nardis, Chiara

AU - Scarpelli, Riccardo

AU - Ade, Paola

PY - 2018/4

Y1 - 2018/4

N2 - In the reported case, a 41-year-old Italian man came to the clinician's observation reporting cramps, bloating and watery diarrhoea a few days after drinking water indicated as unpotable from a fountain in a farm area. The medical suspicion was directed at both gluten intolerance and enteric infection, eventually of waterborne origin. Gluten intolerance was investigated by intestinal biopsy and excluded, while stool analyses ruled out infective bacteriological or viral agents and parasites. Subsequently, a persistent eosinophilia was revealed and a parasitological analysis was again suggested, planning for a more sensitive molecular method. Therefore, a multiplex-PCR of enteric protozoa species DNA was performed on an intestinal biopsy and faecal samples revealing only Blastocystis hominis protozoa, subsequently typed as subtype 1 by RFLP-PCR method. B. hominis is an anaerobic protozoa found in the human and animal intestinal tract, recently associated with a pathogenic role characterized by chronic development. Since blastocystosis has been demonstrated as a waterborne infection, a sample of water matrix was analysed, revealing the B. hominis subtype 1 DNA inside. A probable water transmission of Blastocystis infection has been demonstrated in this case report. Only a probiotic treatment based on Saccharomyces boulardii was administered to the patient and this apparently resolved the infection. In summary, the case described here is a chronic blastocystosis of possible waterborne origin, controlled by assuming a yeast treatment.

AB - In the reported case, a 41-year-old Italian man came to the clinician's observation reporting cramps, bloating and watery diarrhoea a few days after drinking water indicated as unpotable from a fountain in a farm area. The medical suspicion was directed at both gluten intolerance and enteric infection, eventually of waterborne origin. Gluten intolerance was investigated by intestinal biopsy and excluded, while stool analyses ruled out infective bacteriological or viral agents and parasites. Subsequently, a persistent eosinophilia was revealed and a parasitological analysis was again suggested, planning for a more sensitive molecular method. Therefore, a multiplex-PCR of enteric protozoa species DNA was performed on an intestinal biopsy and faecal samples revealing only Blastocystis hominis protozoa, subsequently typed as subtype 1 by RFLP-PCR method. B. hominis is an anaerobic protozoa found in the human and animal intestinal tract, recently associated with a pathogenic role characterized by chronic development. Since blastocystosis has been demonstrated as a waterborne infection, a sample of water matrix was analysed, revealing the B. hominis subtype 1 DNA inside. A probable water transmission of Blastocystis infection has been demonstrated in this case report. Only a probiotic treatment based on Saccharomyces boulardii was administered to the patient and this apparently resolved the infection. In summary, the case described here is a chronic blastocystosis of possible waterborne origin, controlled by assuming a yeast treatment.

KW - Adult

KW - Blastocystis Infections/parasitology

KW - Blastocystis hominis/isolation & purification

KW - Humans

KW - Italy

KW - Male

KW - Probiotics/therapeutic use

KW - Water/parasitology

M3 - Article

C2 - 29498738

VL - 41

SP - 173

EP - 177

JO - New Microbiologica

JF - New Microbiologica

SN - 1121-7138

IS - 2

ER -