Bleeding time in rats: A comparison of different experimental conditions

E. Dejana, S. Villa, G. De Gaetano

Research output: Contribution to journalArticle

169 Citations (Scopus)

Abstract

The tail bleeding time (BT) in rats definitely varies according to the method applied. Of the various variables that may influence BT, we have evaluated the position (horizontal or vertical) of the tail, the environment (air or saline), the temperature (4°, 23° or 37° C) and the type of anaesthesia. Transection of the tail tip cannot be used to screen drugs active on platelet function since it is sensitive to coagulation defects. Template BT in contrast is not modified by heparin and is sensitive to defects of platelet number and function ('storage pool disease', dipyridamole-like drugs, exogenous prostaglandin). In contrast the test fails to detect aspirin-induced platelet dysfunction. The evidence reported indicates that thromboxane A2-prostacyclin balance is not a factor regulating BT. Aspirin treatment however may be a precipitating factor when associated with other abnormalities of platelet function. Template BT is a valid screening test for platelet disorders and for antiplatelet drugs.

Original languageEnglish
Pages (from-to)108-111
Number of pages4
JournalThrombosis and Haemostasis
Volume48
Issue number1
Publication statusPublished - 1982

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Bleeding Time
Blood Platelets
Tail
Aspirin
Precipitating Factors
Thromboxane A2
Dipyridamole
Platelet Aggregation Inhibitors
Epoprostenol
Platelet Count
Pharmaceutical Preparations
Prostaglandins
Heparin
Anesthesia
Air
Temperature

ASJC Scopus subject areas

  • Hematology

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Bleeding time in rats : A comparison of different experimental conditions. / Dejana, E.; Villa, S.; De Gaetano, G.

In: Thrombosis and Haemostasis, Vol. 48, No. 1, 1982, p. 108-111.

Research output: Contribution to journalArticle

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