Blockade of membrane transport and disassembly of the Golgi complex by expression of syntaxin 1A in neurosecretion-incompetent cells

Prevention by rbSEC1

Joanna Rowe, Nicoletta Corradi, Maria Luisa Malosio, Elena Taverna, Philippe Halban, Jacopo Meldolesi, Patrizia Rosa

Research output: Contribution to journalArticle

78 Citations (Scopus)

Abstract

The t-SNAREs syntaxin1A and SNAP-25, i.e. the members of the complex involved in regulated exocytosis at synapses and neurosecretory cells, are delivered to their physiological site, the plasma membrane, when transfected into neurosecretion-competent cells, such as PC12 and AtT20. In contrast, when transfection is made into cells incompetent for neurosecretion, such as those of a defective PC12 clone and the NRK fibroblasts, which have no endogenous expression of these t-SNAREs, syntaxin1A (but neither two other syntaxin family members nor SNAP-25) remains stuck in the Golgi-TGN area with profound consequences to the cell: blockade of both membrane (SNAP-25, GAT-1) and secretory (chromogranin B) protein transport to the cell surface; progressive disassembly of the Golgi complex and TGN; ultimate disappearance of the latter structures, with intermixing of their markers (mannosidase II; TGN-38) with those of the endoplasmic reticulum (calreticulin) and with syntaxin1A itself. When, however, syntaxin 1A is transfected together with rbSec1, a protein known to participate in neurosecretory exocytosis via its dynamic interaction with the t-SNARE, neither the blockade nor the alterations of the Golgi complex take place. Our results demonstrate that syntaxin1A, in addition to its role in exocytosis at the cell surface, possesses a specific potential to interfere with intracellular membrane transport and that its interaction with rbSec1 is instrumental to its physiological function not only at the plasma membrane but also within the cell. At the latter site, the rbSec1-induced conversion of syntaxin1A into a form that can be transported and protects the cell from the development of severe structural and membrane traffic alterations.

Original languageEnglish
Pages (from-to)1865-1877
Number of pages13
JournalJournal of Cell Science
Volume112
Issue number12
Publication statusPublished - 1999

Fingerprint

Syntaxin 1
Neurosecretion
Golgi Apparatus
Membranes
SNARE Proteins
Exocytosis
Chromogranin B
Cell Membrane
Qa-SNARE Proteins
Calreticulin
Intracellular Membranes
Protein Transport
Endoplasmic Reticulum
Synapses
Transfection
Clone Cells
Fibroblasts

Keywords

  • Golgi complex
  • Membrane transport
  • rbSec1/munc18-1
  • SNARE

ASJC Scopus subject areas

  • Cell Biology

Cite this

Blockade of membrane transport and disassembly of the Golgi complex by expression of syntaxin 1A in neurosecretion-incompetent cells : Prevention by rbSEC1. / Rowe, Joanna; Corradi, Nicoletta; Malosio, Maria Luisa; Taverna, Elena; Halban, Philippe; Meldolesi, Jacopo; Rosa, Patrizia.

In: Journal of Cell Science, Vol. 112, No. 12, 1999, p. 1865-1877.

Research output: Contribution to journalArticle

Rowe, Joanna ; Corradi, Nicoletta ; Malosio, Maria Luisa ; Taverna, Elena ; Halban, Philippe ; Meldolesi, Jacopo ; Rosa, Patrizia. / Blockade of membrane transport and disassembly of the Golgi complex by expression of syntaxin 1A in neurosecretion-incompetent cells : Prevention by rbSEC1. In: Journal of Cell Science. 1999 ; Vol. 112, No. 12. pp. 1865-1877.
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T1 - Blockade of membrane transport and disassembly of the Golgi complex by expression of syntaxin 1A in neurosecretion-incompetent cells

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AU - Taverna, Elena

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AU - Meldolesi, Jacopo

AU - Rosa, Patrizia

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AB - The t-SNAREs syntaxin1A and SNAP-25, i.e. the members of the complex involved in regulated exocytosis at synapses and neurosecretory cells, are delivered to their physiological site, the plasma membrane, when transfected into neurosecretion-competent cells, such as PC12 and AtT20. In contrast, when transfection is made into cells incompetent for neurosecretion, such as those of a defective PC12 clone and the NRK fibroblasts, which have no endogenous expression of these t-SNAREs, syntaxin1A (but neither two other syntaxin family members nor SNAP-25) remains stuck in the Golgi-TGN area with profound consequences to the cell: blockade of both membrane (SNAP-25, GAT-1) and secretory (chromogranin B) protein transport to the cell surface; progressive disassembly of the Golgi complex and TGN; ultimate disappearance of the latter structures, with intermixing of their markers (mannosidase II; TGN-38) with those of the endoplasmic reticulum (calreticulin) and with syntaxin1A itself. When, however, syntaxin 1A is transfected together with rbSec1, a protein known to participate in neurosecretory exocytosis via its dynamic interaction with the t-SNARE, neither the blockade nor the alterations of the Golgi complex take place. Our results demonstrate that syntaxin1A, in addition to its role in exocytosis at the cell surface, possesses a specific potential to interfere with intracellular membrane transport and that its interaction with rbSec1 is instrumental to its physiological function not only at the plasma membrane but also within the cell. At the latter site, the rbSec1-induced conversion of syntaxin1A into a form that can be transported and protects the cell from the development of severe structural and membrane traffic alterations.

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