Blocked signal transduction to the ERK and JNK protein kinases in anergic CD4+ T cells

Wei Li, Carmella D. Whaley, Anna Mondino, Daniel L. Mueller

Research output: Contribution to journalArticle

397 Citations (Scopus)

Abstract

T cells activated by antigen receptor stimulation in the absence of accessory cell-derived costimulatory signals lose the capacity to synthesize the growth factor interleukin-2 (IL-2), a state called clonal anergy. An analysis of CD3- and CD28-induced signal transduction revealed reduced ERK and JNK enzyme activities in murine anergic T cells. The amounts of ERK and JNK proteins were unchanged, and the kinases could be fully activated in the presence of phorbol 12-myristate 13-acetate. Dephosphorylation of the calcineurin substrate NFATp (preexisting nuclear factor of activated T cells) also remained inducible. These results suggest that a specific block in the activation of ERK and JNK contributes to defective IL-2 production in clonal anergy.

Original languageEnglish
Pages (from-to)1272-1276
Number of pages5
JournalScience
Volume271
Issue number5253
Publication statusPublished - Mar 1 1996

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Clonal Anergy
MAP Kinase Kinase 4
Protein Kinases
Interleukin-2
Signal Transduction
NFATC Transcription Factors
T-Lymphocytes
Calcineurin
T-Cell Antigen Receptor
Intercellular Signaling Peptides and Proteins
Acetates
Phosphotransferases
Enzymes
Proteins

ASJC Scopus subject areas

  • General

Cite this

Li, W., Whaley, C. D., Mondino, A., & Mueller, D. L. (1996). Blocked signal transduction to the ERK and JNK protein kinases in anergic CD4+ T cells. Science, 271(5253), 1272-1276.

Blocked signal transduction to the ERK and JNK protein kinases in anergic CD4+ T cells. / Li, Wei; Whaley, Carmella D.; Mondino, Anna; Mueller, Daniel L.

In: Science, Vol. 271, No. 5253, 01.03.1996, p. 1272-1276.

Research output: Contribution to journalArticle

Li, W, Whaley, CD, Mondino, A & Mueller, DL 1996, 'Blocked signal transduction to the ERK and JNK protein kinases in anergic CD4+ T cells', Science, vol. 271, no. 5253, pp. 1272-1276.
Li, Wei ; Whaley, Carmella D. ; Mondino, Anna ; Mueller, Daniel L. / Blocked signal transduction to the ERK and JNK protein kinases in anergic CD4+ T cells. In: Science. 1996 ; Vol. 271, No. 5253. pp. 1272-1276.
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