Blood-brain barrier preservation in the in vitro isolated guinea pig brain preparation

Laura Librizzi, Damir Janigro, Silvia De Biasi, Marco De Curtis

Research output: Contribution to journalArticle

Abstract

The morphofunctional preservation of the blood-brain barrier (BBB) was evaluated in the isolated guinea pig brain maintained in vitro by arterial perfusion. Electron microscopy evaluation after 5 hr in vitro demonstrated that cerebral capillaries and BBB specializations in this preparation retain features compatible with structural integrity. BBB-impermeable and -permeable atropine derivatives arterially perfused to antagonize carbachol-induced fast oscillatory activity confirmed the functional preservation of the BBB in vitro. To study BBB function further, changes in extracellular K+ concentration during arterial perfusion of a high-K+ solution were measured with K+-sensitive electrodes positioned in the cortex and, as control, at the brain venous outlet, where the solution perfused through the brain arterial system was collected. After 5 hr in vitro, the [K+]o values measured during high-K+ perfusion in the piriform and entorhinal cortices were 5.02±0.17 mM (mean±SE) and 5.2±0.21 mM, respectively (n = 6). Coperfusion of the high-K+ solution with the Na+/K+ pump blocker ouabain (10μM;n=4) induced consistently spreading depression preceded by a rise in [K+]o. Finally, sporadic, isolated spots of extravasation of the fluorescent marker fluorescein isothiocyanate (FITC)-dextran preferentially circumscribed to deep cortical layers was observed in brains perfused with FITC-dextran after 5 hr in vitro. The study demonstrates that the in vitro isolated guinea pig brain is viable for studying cerebrovascular interactions and BBB permeability of compounds active in the central nervous system.

Original languageEnglish
Pages (from-to)289-297
Number of pages9
JournalJournal of Neuroscience Research
Volume66
Issue number2
DOIs
Publication statusPublished - Oct 15 2001

Keywords

  • Cerebrovascular interactions
  • Cortex
  • Endothelial cells
  • Isolated in vitro brain
  • Potassium

ASJC Scopus subject areas

  • Neuroscience(all)

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