Blood cell counting in neonates: A comparison between a low volume micromethod and the standard laboratory method

Fabrizio Papa, Mauro Rongioletti, Marco Della Ventura, Francesco Di Turi, Maurizio Cortesi, Patrizio Pasqualetti, Maria Bernardetta Majolini, Valeria Collegiani, Marika Cicchese, Maria Letizia Notarmuzi, Rocco Agostino, Giancarlo Maria Liumbruno

Research output: Contribution to journalArticlepeer-review


Background. Iatrogenic anaemia caused by repeated blood sampling to monitor laboratory parameters can contribute, particularly in neonates, to the need for transfusion. "Point of care" laboratory equipment uses smaller amounts of blood for analytic determinations and could, therefore, help to prevent secondary anaemia. In this study we compared the results of haematological parameters measured using a standard laboratory method and using a "point of care" micromethod, with the aim of validating the use of this latter method in clinical practice in neonatology. Materials and methods. One hundred and fifty venous or capillary blood samples were taken from full-term or premature neonates 2-4 hours or 48 hours after birth. Each sample was processed by a standard haematology analyser and another micromethod instrument. Bland- Altman plots were constructed for each parameter and intra-class coefficients of correlation were calculated in order to evaluate the concordance between the two analysers. Results. The concordance between the data obtained with the two analysers, expressed as the intra-class correlation, was 0.98 for white blood cell count, 0.97 for haemoglobin concentration, 0.96 for haematocrit, 0.95 for mean red cell volume and 0.98 for platelet count. The micromethod produced overestimated mean values for the leucocyte count (+1.27; p

Original languageEnglish
Pages (from-to)400-406
Number of pages7
JournalBlood Transfusion
Issue number4
Publication statusPublished - 2011


  • Neonatal anaemia
  • Neonatal thrombocytopenia
  • Neonatology
  • Point of care
  • Transfusion trigger

ASJC Scopus subject areas

  • Hematology
  • Immunology and Allergy


Dive into the research topics of 'Blood cell counting in neonates: A comparison between a low volume micromethod and the standard laboratory method'. Together they form a unique fingerprint.

Cite this