TY - JOUR
T1 - Blood cell counting in neonates
T2 - A comparison between a low volume micromethod and the standard laboratory method
AU - Papa, Fabrizio
AU - Rongioletti, Mauro
AU - Della Ventura, Marco
AU - Di Turi, Francesco
AU - Cortesi, Maurizio
AU - Pasqualetti, Patrizio
AU - Majolini, Maria Bernardetta
AU - Collegiani, Valeria
AU - Cicchese, Marika
AU - Notarmuzi, Maria Letizia
AU - Agostino, Rocco
AU - Liumbruno, Giancarlo Maria
PY - 2011
Y1 - 2011
N2 - Background. Iatrogenic anaemia caused by repeated blood sampling to monitor laboratory parameters can contribute, particularly in neonates, to the need for transfusion. "Point of care" laboratory equipment uses smaller amounts of blood for analytic determinations and could, therefore, help to prevent secondary anaemia. In this study we compared the results of haematological parameters measured using a standard laboratory method and using a "point of care" micromethod, with the aim of validating the use of this latter method in clinical practice in neonatology. Materials and methods. One hundred and fifty venous or capillary blood samples were taken from full-term or premature neonates 2-4 hours or 48 hours after birth. Each sample was processed by a standard haematology analyser and another micromethod instrument. Bland- Altman plots were constructed for each parameter and intra-class coefficients of correlation were calculated in order to evaluate the concordance between the two analysers. Results. The concordance between the data obtained with the two analysers, expressed as the intra-class correlation, was 0.98 for white blood cell count, 0.97 for haemoglobin concentration, 0.96 for haematocrit, 0.95 for mean red cell volume and 0.98 for platelet count. The micromethod produced overestimated mean values for the leucocyte count (+1.27; p
AB - Background. Iatrogenic anaemia caused by repeated blood sampling to monitor laboratory parameters can contribute, particularly in neonates, to the need for transfusion. "Point of care" laboratory equipment uses smaller amounts of blood for analytic determinations and could, therefore, help to prevent secondary anaemia. In this study we compared the results of haematological parameters measured using a standard laboratory method and using a "point of care" micromethod, with the aim of validating the use of this latter method in clinical practice in neonatology. Materials and methods. One hundred and fifty venous or capillary blood samples were taken from full-term or premature neonates 2-4 hours or 48 hours after birth. Each sample was processed by a standard haematology analyser and another micromethod instrument. Bland- Altman plots were constructed for each parameter and intra-class coefficients of correlation were calculated in order to evaluate the concordance between the two analysers. Results. The concordance between the data obtained with the two analysers, expressed as the intra-class correlation, was 0.98 for white blood cell count, 0.97 for haemoglobin concentration, 0.96 for haematocrit, 0.95 for mean red cell volume and 0.98 for platelet count. The micromethod produced overestimated mean values for the leucocyte count (+1.27; p
KW - Neonatal anaemia
KW - Neonatal thrombocytopenia
KW - Neonatology
KW - Point of care
KW - Transfusion trigger
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U2 - 10.2450/2011.0082-10
DO - 10.2450/2011.0082-10
M3 - Article
C2 - 21839016
AN - SCOPUS:80054957239
VL - 9
SP - 400
EP - 406
JO - Blood Transfusion
JF - Blood Transfusion
SN - 1723-2007
IS - 4
ER -