Blood coagulation changes in nude mice bearing human colon carcinomas

M. R. Bani, A. Falanga, M. G. Alessio, E. Radice, R. Consonni, R. Giavazzi, M. B. Donati

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Abstract

We studied several blood coagulation parameters and tumor tissue procoagulant activity (PCA) in nude mice bearing human colorectal carcinomas (HCC). In a control group of 51 tumor-free nude mice, platelet number was 1.2 ± 0.03 x 106/μl, thrombotest activity 90% ± 2.6 and fibrinogen 172 ± 11 mg/dl. The same parameters were studied in nude mice (n = 71) bearing 7 different HCC lines subcutaneously (s.c.). The results did not significantly differ from those in control mice but there was broad variability among groups of mice injected with different HCC lines, ranging from 0.36 to 2.55 x 106/μl for platelets, from 100 to 28% for thrombotest activity and from 42 to 460 mg/dl for fibrinogen. The results were significantly (p <0.05) different from those in the tumor-free group when each group of HCC-bearing animals was analyzed individually. A malignant HCC line that grew in the liver of nude mice (n = 24) significantly (p <0.001) reduced thrombotest activity (58% ± 5.9). The PCA of tisse extracts from tumors grown s.c. in nude mice was assayed. All the HCC xenografts expressed PCA which differed significantly for the various tumor lines (from 25.5 ± 1.9 to 2.8 ± 0.6 unit/mg in tumor tissue). Cancer procoagulant (CP), a cysteine proteinase with a direct factor-X-activating effect, was present in different amounts (84.7 ± 4.3 to 59.5 ± 9.0%) in the tumors. Our results indicates that the nude mouse is a suitable model for evaluating the hemostatic changes induced by human tumors and may represent a tool for investigating the underlying biochemical mechanisms.

Original languageEnglish
Pages (from-to)75-79
Number of pages5
JournalInternational Journal of Cancer
Volume50
Issue number1
Publication statusPublished - 1992

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Blood Coagulation
Nude Mice
Colon
Carcinoma
Colorectal Neoplasms
Prothrombin Time
Neoplasms
cancer procoagulant
Fibrinogen
Factor X
Cysteine Proteases
Hemostatics
Platelet Count
Heterografts
Blood Platelets
Control Groups
Liver

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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Blood coagulation changes in nude mice bearing human colon carcinomas. / Bani, M. R.; Falanga, A.; Alessio, M. G.; Radice, E.; Consonni, R.; Giavazzi, R.; Donati, M. B.

In: International Journal of Cancer, Vol. 50, No. 1, 1992, p. 75-79.

Research output: Contribution to journalArticle

Bani, M. R. ; Falanga, A. ; Alessio, M. G. ; Radice, E. ; Consonni, R. ; Giavazzi, R. ; Donati, M. B. / Blood coagulation changes in nude mice bearing human colon carcinomas. In: International Journal of Cancer. 1992 ; Vol. 50, No. 1. pp. 75-79.
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