Blood-Derived Stem Cells (BDSCs) plasticity: In vitro hepatic differentiation

Giorgia Alaimo, Eliana Cozzoli, Gabriella Marfe, Luca Esposito, Marco Ranalli, Dalya Hmada, Antonio Giordano, Alessandra Gambacurta

Research output: Contribution to journalArticle

Abstract

The limited availability of hepatic tissue suitable for the treatment of liver disease and drug research encourages the generation of hepatic-like cells from alternative sources as support for the regenerative medicine. Human blood derived stem cells (BDSCs) express surface markers and genes characteristic of pluripotent stem cells and have the ability to differentiate into different cell types, including tissues of endodermal origin (i.e., liver). Therefore they can represent a valuable source of hepatocytes for medicine. In this investigation, we exploited a fast hepatic differentiation protocol to generate hepatocyte-like cells from human BDSCs using only hepatocyte growth factor (HGF) and fibroblast growth factor-4 (FGF-4) as growth factors. The resulting cell population exhibited hepatic cell-like morphology and it was characterized with a variety of biological endpoint analyses. Here, we demonstrate how human BDSCs can be reprogrammed in hepatocyte-like cells by morphological, functional analysis, reverse transcriptase (RT)-PCR, and Western Blot assay. This study defines a fast and easy reprogramming strategy that facilitates the differentiation of human BDSCs along a hepatic lineage and provides a framework for a helpful source in the stem cells therapy and liver disorders.

Original languageEnglish
Pages (from-to)1249-1254
Number of pages6
JournalJournal of Cellular Physiology
Volume228
Issue number6
DOIs
Publication statusPublished - Jun 2013

Fingerprint

Stem cells
Plasticity
Hepatocytes
Blood
Stem Cells
Liver
Fibroblast Growth Factor 4
Pluripotent Stem Cells
Regenerative Medicine
Hepatocyte Growth Factor
Tissue
Functional analysis
Cell- and Tissue-Based Therapy
Reverse Transcriptase Polymerase Chain Reaction
RNA-Directed DNA Polymerase
Liver Diseases
Blood Cells
Intercellular Signaling Peptides and Proteins
Western Blotting
Medicine

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology
  • Physiology

Cite this

Alaimo, G., Cozzoli, E., Marfe, G., Esposito, L., Ranalli, M., Hmada, D., ... Gambacurta, A. (2013). Blood-Derived Stem Cells (BDSCs) plasticity: In vitro hepatic differentiation. Journal of Cellular Physiology, 228(6), 1249-1254. https://doi.org/10.1002/jcp.24279

Blood-Derived Stem Cells (BDSCs) plasticity : In vitro hepatic differentiation. / Alaimo, Giorgia; Cozzoli, Eliana; Marfe, Gabriella; Esposito, Luca; Ranalli, Marco; Hmada, Dalya; Giordano, Antonio; Gambacurta, Alessandra.

In: Journal of Cellular Physiology, Vol. 228, No. 6, 06.2013, p. 1249-1254.

Research output: Contribution to journalArticle

Alaimo, G, Cozzoli, E, Marfe, G, Esposito, L, Ranalli, M, Hmada, D, Giordano, A & Gambacurta, A 2013, 'Blood-Derived Stem Cells (BDSCs) plasticity: In vitro hepatic differentiation', Journal of Cellular Physiology, vol. 228, no. 6, pp. 1249-1254. https://doi.org/10.1002/jcp.24279
Alaimo G, Cozzoli E, Marfe G, Esposito L, Ranalli M, Hmada D et al. Blood-Derived Stem Cells (BDSCs) plasticity: In vitro hepatic differentiation. Journal of Cellular Physiology. 2013 Jun;228(6):1249-1254. https://doi.org/10.1002/jcp.24279
Alaimo, Giorgia ; Cozzoli, Eliana ; Marfe, Gabriella ; Esposito, Luca ; Ranalli, Marco ; Hmada, Dalya ; Giordano, Antonio ; Gambacurta, Alessandra. / Blood-Derived Stem Cells (BDSCs) plasticity : In vitro hepatic differentiation. In: Journal of Cellular Physiology. 2013 ; Vol. 228, No. 6. pp. 1249-1254.
@article{6eeca79139fe490eb2a86d1374ea0ebe,
title = "Blood-Derived Stem Cells (BDSCs) plasticity: In vitro hepatic differentiation",
abstract = "The limited availability of hepatic tissue suitable for the treatment of liver disease and drug research encourages the generation of hepatic-like cells from alternative sources as support for the regenerative medicine. Human blood derived stem cells (BDSCs) express surface markers and genes characteristic of pluripotent stem cells and have the ability to differentiate into different cell types, including tissues of endodermal origin (i.e., liver). Therefore they can represent a valuable source of hepatocytes for medicine. In this investigation, we exploited a fast hepatic differentiation protocol to generate hepatocyte-like cells from human BDSCs using only hepatocyte growth factor (HGF) and fibroblast growth factor-4 (FGF-4) as growth factors. The resulting cell population exhibited hepatic cell-like morphology and it was characterized with a variety of biological endpoint analyses. Here, we demonstrate how human BDSCs can be reprogrammed in hepatocyte-like cells by morphological, functional analysis, reverse transcriptase (RT)-PCR, and Western Blot assay. This study defines a fast and easy reprogramming strategy that facilitates the differentiation of human BDSCs along a hepatic lineage and provides a framework for a helpful source in the stem cells therapy and liver disorders.",
author = "Giorgia Alaimo and Eliana Cozzoli and Gabriella Marfe and Luca Esposito and Marco Ranalli and Dalya Hmada and Antonio Giordano and Alessandra Gambacurta",
year = "2013",
month = "6",
doi = "10.1002/jcp.24279",
language = "English",
volume = "228",
pages = "1249--1254",
journal = "Journal of cellular and comparative physiology",
issn = "0021-9541",
publisher = "Wiley-Liss Inc.",
number = "6",

}

TY - JOUR

T1 - Blood-Derived Stem Cells (BDSCs) plasticity

T2 - In vitro hepatic differentiation

AU - Alaimo, Giorgia

AU - Cozzoli, Eliana

AU - Marfe, Gabriella

AU - Esposito, Luca

AU - Ranalli, Marco

AU - Hmada, Dalya

AU - Giordano, Antonio

AU - Gambacurta, Alessandra

PY - 2013/6

Y1 - 2013/6

N2 - The limited availability of hepatic tissue suitable for the treatment of liver disease and drug research encourages the generation of hepatic-like cells from alternative sources as support for the regenerative medicine. Human blood derived stem cells (BDSCs) express surface markers and genes characteristic of pluripotent stem cells and have the ability to differentiate into different cell types, including tissues of endodermal origin (i.e., liver). Therefore they can represent a valuable source of hepatocytes for medicine. In this investigation, we exploited a fast hepatic differentiation protocol to generate hepatocyte-like cells from human BDSCs using only hepatocyte growth factor (HGF) and fibroblast growth factor-4 (FGF-4) as growth factors. The resulting cell population exhibited hepatic cell-like morphology and it was characterized with a variety of biological endpoint analyses. Here, we demonstrate how human BDSCs can be reprogrammed in hepatocyte-like cells by morphological, functional analysis, reverse transcriptase (RT)-PCR, and Western Blot assay. This study defines a fast and easy reprogramming strategy that facilitates the differentiation of human BDSCs along a hepatic lineage and provides a framework for a helpful source in the stem cells therapy and liver disorders.

AB - The limited availability of hepatic tissue suitable for the treatment of liver disease and drug research encourages the generation of hepatic-like cells from alternative sources as support for the regenerative medicine. Human blood derived stem cells (BDSCs) express surface markers and genes characteristic of pluripotent stem cells and have the ability to differentiate into different cell types, including tissues of endodermal origin (i.e., liver). Therefore they can represent a valuable source of hepatocytes for medicine. In this investigation, we exploited a fast hepatic differentiation protocol to generate hepatocyte-like cells from human BDSCs using only hepatocyte growth factor (HGF) and fibroblast growth factor-4 (FGF-4) as growth factors. The resulting cell population exhibited hepatic cell-like morphology and it was characterized with a variety of biological endpoint analyses. Here, we demonstrate how human BDSCs can be reprogrammed in hepatocyte-like cells by morphological, functional analysis, reverse transcriptase (RT)-PCR, and Western Blot assay. This study defines a fast and easy reprogramming strategy that facilitates the differentiation of human BDSCs along a hepatic lineage and provides a framework for a helpful source in the stem cells therapy and liver disorders.

UR - http://www.scopus.com/inward/record.url?scp=84874212963&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84874212963&partnerID=8YFLogxK

U2 - 10.1002/jcp.24279

DO - 10.1002/jcp.24279

M3 - Article

C2 - 23139117

AN - SCOPUS:84874212963

VL - 228

SP - 1249

EP - 1254

JO - Journal of cellular and comparative physiology

JF - Journal of cellular and comparative physiology

SN - 0021-9541

IS - 6

ER -