Blood glutathione as a marker of cardiac allograft vasculopathy in heart transplant recipients

Benedetta De Chiara, Riccardo Bigi, Jonica Campolo, Marina Parolini, Fabio Turazza, Gabriella Masciocco, Maria Frigerio, Cesare Fiorentini, Oberdan Parodi

Research output: Contribution to journalArticlepeer-review

Abstract

Background: Cardiac allograft vasculopathy (CAV) limits survival after heart transplantation (HTx). Between immunologic and non-immunologic factors, reactive oxygen species generation has been proposed as pathogenetic mechanism. This study was aimed at evaluating redox status in HTx recipients and verifying whether it could be independently associated with CAV. Methods: Fifty-five consecutive male HTx recipients, median [interquartile range] age 60 yr [50, 64], underwent angiography 67 months [21, 97] after HTx to assess CAV, defined as significant stenosis in ≥1 epicardial vessel or any distal vessel attenuation. All patients underwent blood sampling 89 months [67, 119] after HTx for biochemical (glucose, creatinine, total and LDL cholesterol, and cyclosporin levels) and redox evaluation [plasma reduced and total homocysteine, cysteine, cysteinylglycine, glutathione, blood reduced glutathione (GSHbl) and vitamin E]. Univariate Odds Ratios (OR) with 95% confidence interval (95% CI, highest vs. lowest quartile) were estimated on the basis of a logistic regression analysis between clinical, conventional biochemical and redox data. Only the significant variables at univariate entered into multivariate analysis. Results: CAV was documented in 15 (27%) patients. Univariate analysis showed that time from HTx to angiography (OR 3.97, 95% CI 1.15-14, p = 0.03) and GSHbl (OR 0.31, 95% CI: 0.14-0.70, p = 0.005) were significantly associated with CAV. However, multivariate analysis revealed GSHbl as the only independent predictor of CAV (OR 0.31, 95% CI: 0.13-0.74, p = 0.008). Conclusions: In HTx recipients reduced levels of GSHbl are independently associated with CAV. Given its potent intracellular scavenger properties, GSHbl may serve as a marker of antioxidant defence consumption, favouring CAV development.

Original languageEnglish
Pages (from-to)367-371
Number of pages5
JournalClinical Transplantation
Volume19
Issue number3
DOIs
Publication statusPublished - Jun 2005

Keywords

  • Antioxidants
  • Cardiac allograft vasculopathy
  • Free radicals
  • Glutathione
  • Heart transplantation

ASJC Scopus subject areas

  • Transplantation
  • Immunology

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