Blood group genotyping for Jka/Jkb, Fy a/Fyb, S/s, K/k, Kpa/Kpb, Js a/Jsb, Coa/Cob, and Lu a/Lub with microarray beads

Katerina Karpasitou, Francesca Drago, Loretta Crespiatico, Cinzia Paccapelo, Francesca Truglio, Sara Frison, Mario Scalamogna, Francesca Poli

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Abstract

BACKGROUND: Traditionally, blood group typing has been performed with serologic techniques, the classical method being the hemagglutination test. Serotyping, however, may present important limitations such as scarce availability of rare antisera, typing of recently transfused patients, and those with a positive direct antiglobulin test. Consequently, serologic tests are being complemented with molecular methods. The aim of this study was to develop a low-cost, high-throughput method for large-scale genotyping of red blood cells (RBCs). STUDY DESIGN AND METHODS: Single-nucleotide polymorphisms associated with some clinically important blood group antigens, as well as with certain rare blood antigens, were evaluated: Jka/Jkb, Fy a/Fyb, S/s, K/k, Kpa/Kpb, Js a/Jsb, Coa/Cob, and Lu a/Lub. Polymerase chain reaction (PCR)-amplified targets were detected by direct hybridization to microspheres coupled to allele-specific oligonucleotides. Cutoff values for each genotype were established with phenotyped and/or genotyped samples. RESULTS: The method was validated with a blind panel of 92 blood donor samples. The results were fully concordant with those provided by hemagglutination assays and/or sequence-specific primer (SSP)-PCR. The method was subsequently evaluated with approximately 800 blood donor and patient samples. CONCLUSION: This study presents a flexible, quick, and economical method for complete genotyping of large donor cohorts for RBC alleles.

Original languageEnglish
Pages (from-to)505-512
Number of pages8
JournalTransfusion
Volume48
Issue number3
DOIs
Publication statusPublished - Mar 2008

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ASJC Scopus subject areas

  • Hematology
  • Immunology

Cite this

Karpasitou, K., Drago, F., Crespiatico, L., Paccapelo, C., Truglio, F., Frison, S., Scalamogna, M., & Poli, F. (2008). Blood group genotyping for Jka/Jkb, Fy a/Fyb, S/s, K/k, Kpa/Kpb, Js a/Jsb, Coa/Cob, and Lu a/Lub with microarray beads. Transfusion, 48(3), 505-512. https://doi.org/10.1111/j.1537-2995.2007.01555.x