Blood orange juice inhibits fat accumulation in mice

L. Titta, M. Trinei, M. Stendardo, I. Berniakovich, K. Petroni, C. Tonelli, P. Riso, M. Porrini, S. Minucci, P. G. Pelicci, P. Rapisarda, G. Reforgiato Recupero, M. Giorgio

Research output: Contribution to journalArticle

94 Citations (Scopus)

Abstract

Objective:To analyze the effect of the juice obtained from two varieties of sweet orange (Citrus sinensis L. Osbeck), Moro (a blood orange) and Navelina (a blond orange), on fat accumulation in mice fed a standard or a high-fat diet (HFD).Methods:Obesity was induced in male C57/Bl6 mice by feeding a HFD. Moro and Navelina juices were provided instead of water. The effect of an anthocyanin-enriched extract from Moro oranges or purified cyanidin-3-glucoside (C3G) was also analyzed. Body weight and food intake were measured regularly over a 12-week period. The adipose pads were weighted and analyzed histologically; total RNA was also isolated for microarray analysis.Results: Dietary supplementation of Moro juice, but not Navelina juice significantly reduced body weight gain and fat accumulation regardless of the increased energy intake because of sugar content. Furthermore, mice drinking Moro juice were resistant to HFD-induced obesity with no alterations in food intake. Only the anthocyanin extract, but not the purified C3G, slightly affected fat accumulation. High-throughput gene expression analysis of fat tissues confirmed that Moro juice could entirely rescue the high fat-induced transcriptional reprogramming.Conclusion:Moro juice anti-obesity effect on fat accumulation cannot be explained only by its anthocyanin content. Our findings suggest that multiple components present in the Moro orange juice might act synergistically to inhibit fat accumulation.

Original languageEnglish
Pages (from-to)578-588
Number of pages11
JournalInternational Journal of Obesity
Volume34
Issue number3
DOIs
Publication statusPublished - Mar 2010

Fingerprint

Fats
Anthocyanins
High Fat Diet
Obesity
Eating
Body Weight
Microarray Analysis
Dietary Supplements
Energy Intake
Drinking
Weight Gain
Adipose Tissue
RNA
Gene Expression
Water
cyanidin 3-O-glucoside

Keywords

  • Anthocyanins
  • Anti-obesity
  • Blood orange
  • Cyanidin-3-glucoside
  • Fat accumulation
  • Orange juice

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Nutrition and Dietetics
  • Endocrinology, Diabetes and Metabolism
  • Medicine(all)

Cite this

Blood orange juice inhibits fat accumulation in mice. / Titta, L.; Trinei, M.; Stendardo, M.; Berniakovich, I.; Petroni, K.; Tonelli, C.; Riso, P.; Porrini, M.; Minucci, S.; Pelicci, P. G.; Rapisarda, P.; Recupero, G. Reforgiato; Giorgio, M.

In: International Journal of Obesity, Vol. 34, No. 3, 03.2010, p. 578-588.

Research output: Contribution to journalArticle

Titta, L, Trinei, M, Stendardo, M, Berniakovich, I, Petroni, K, Tonelli, C, Riso, P, Porrini, M, Minucci, S, Pelicci, PG, Rapisarda, P, Recupero, GR & Giorgio, M 2010, 'Blood orange juice inhibits fat accumulation in mice', International Journal of Obesity, vol. 34, no. 3, pp. 578-588. https://doi.org/10.1038/ijo.2009.266
Titta, L. ; Trinei, M. ; Stendardo, M. ; Berniakovich, I. ; Petroni, K. ; Tonelli, C. ; Riso, P. ; Porrini, M. ; Minucci, S. ; Pelicci, P. G. ; Rapisarda, P. ; Recupero, G. Reforgiato ; Giorgio, M. / Blood orange juice inhibits fat accumulation in mice. In: International Journal of Obesity. 2010 ; Vol. 34, No. 3. pp. 578-588.
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