Blood transfusions with high levels ofcontaminating soluble HLA-I correlate with levels of soluble CD8 in recipients' plasma; A new control factor in soluble HLA-I-mediated transfusion-modulated immunomodulation?

Massimo Ghio, Paola Contini, Gianluca Ubezio, Filippo Ansaldi, Maurizio Setti, Gino Tripodi

Research output: Contribution to journalArticlepeer-review

Abstract

Background. The cause of transfusion-related immunomodulation (TRIM) has proved tantalisingly elusive. An ever-growing body of evidence indicates that the infusion of large amounts of soluble and cell-associated antigens into a recipient can somehow induce TRIM. One soluble molecule that has been implicated in TRIM is soluble human leucocyte antigen I (sHLA-I). However, patients infused with large amounts of sHLA-I do not always and unambiguously experience TRIM. As soluble CD8 (sCD8) molecules have been shown to capable of binding membrane and soluble HLA-I molecules, we focused on sCD8 as a possible modulator of sHLA-I-mediated TRIM. Material and methods. To this aim we compared the up-regulation of circulating sCD8 in plasma from patients suffering from the same pathology, but chronically transfused with two different blood derivatives: pre- and post-storage leucodepleted red blood cells which contain low and high levels of contaminating sHLA-I, respectively. Results. Significantly larger amounts of sCD8 circulating molecules were detectable in the plasma of patients transfused with post-storage leucodepleted red blood cells whose supernatants contained significantly larger amounts of sHLA-I contaminating molecules. Conclusion. With the limitation of indirect evidence, this report introduces a new facet of the bioactivity of sCD8 as a possible modulator of sHLA-I-mediated TRIM.

Original languageEnglish
JournalBlood Transfusion
Volume12
Issue numberSUPPL.1
DOIs
Publication statusPublished - 2014

Keywords

  • SCD8.
  • SHLA-I
  • TRIM

ASJC Scopus subject areas

  • Hematology
  • Immunology and Allergy

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