Abstract
Background. The cause of transfusion-related immunomodulation (TRIM) has proved tantalisingly elusive. An ever-growing body of evidence indicates that the infusion of large amounts of soluble and cell-associated antigens into a recipient can somehow induce TRIM. One soluble molecule that has been implicated in TRIM is soluble human leucocyte antigen I (sHLA-I). However, patients infused with large amounts of sHLA-I do not always and unambiguously experience TRIM. As soluble CD8 (sCD8) molecules have been shown to capable of binding membrane and soluble HLA-I molecules, we focused on sCD8 as a possible modulator of sHLA-I-mediated TRIM. Material and methods. To this aim we compared the up-regulation of circulating sCD8 in plasma from patients suffering from the same pathology, but chronically transfused with two different blood derivatives: pre- and post-storage leucodepleted red blood cells which contain low and high levels of contaminating sHLA-I, respectively. Results. Significantly larger amounts of sCD8 circulating molecules were detectable in the plasma of patients transfused with post-storage leucodepleted red blood cells whose supernatants contained significantly larger amounts of sHLA-I contaminating molecules. Conclusion. With the limitation of indirect evidence, this report introduces a new facet of the bioactivity of sCD8 as a possible modulator of sHLA-I-mediated TRIM.
| Original language | English |
|---|---|
| Journal | Blood Transfusion |
| Volume | 12 |
| Issue number | SUPPL.1 |
| DOIs | |
| Publication status | Published - 2014 |
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Keywords
- SCD8.
- SHLA-I
- TRIM
ASJC Scopus subject areas
- Hematology
- Immunology and Allergy
Cite this
Blood transfusions with high levels ofcontaminating soluble HLA-I correlate with levels of soluble CD8 in recipients' plasma; A new control factor in soluble HLA-I-mediated transfusion-modulated immunomodulation? / Ghio, Massimo; Contini, Paola; Ubezio, Gianluca; Ansaldi, Filippo; Setti, Maurizio; Tripodi, Gino.
In: Blood Transfusion, Vol. 12, No. SUPPL.1, 2014.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Blood transfusions with high levels ofcontaminating soluble HLA-I correlate with levels of soluble CD8 in recipients' plasma; A new control factor in soluble HLA-I-mediated transfusion-modulated immunomodulation?
AU - Ghio, Massimo
AU - Contini, Paola
AU - Ubezio, Gianluca
AU - Ansaldi, Filippo
AU - Setti, Maurizio
AU - Tripodi, Gino
PY - 2014
Y1 - 2014
N2 - Background. The cause of transfusion-related immunomodulation (TRIM) has proved tantalisingly elusive. An ever-growing body of evidence indicates that the infusion of large amounts of soluble and cell-associated antigens into a recipient can somehow induce TRIM. One soluble molecule that has been implicated in TRIM is soluble human leucocyte antigen I (sHLA-I). However, patients infused with large amounts of sHLA-I do not always and unambiguously experience TRIM. As soluble CD8 (sCD8) molecules have been shown to capable of binding membrane and soluble HLA-I molecules, we focused on sCD8 as a possible modulator of sHLA-I-mediated TRIM. Material and methods. To this aim we compared the up-regulation of circulating sCD8 in plasma from patients suffering from the same pathology, but chronically transfused with two different blood derivatives: pre- and post-storage leucodepleted red blood cells which contain low and high levels of contaminating sHLA-I, respectively. Results. Significantly larger amounts of sCD8 circulating molecules were detectable in the plasma of patients transfused with post-storage leucodepleted red blood cells whose supernatants contained significantly larger amounts of sHLA-I contaminating molecules. Conclusion. With the limitation of indirect evidence, this report introduces a new facet of the bioactivity of sCD8 as a possible modulator of sHLA-I-mediated TRIM.
AB - Background. The cause of transfusion-related immunomodulation (TRIM) has proved tantalisingly elusive. An ever-growing body of evidence indicates that the infusion of large amounts of soluble and cell-associated antigens into a recipient can somehow induce TRIM. One soluble molecule that has been implicated in TRIM is soluble human leucocyte antigen I (sHLA-I). However, patients infused with large amounts of sHLA-I do not always and unambiguously experience TRIM. As soluble CD8 (sCD8) molecules have been shown to capable of binding membrane and soluble HLA-I molecules, we focused on sCD8 as a possible modulator of sHLA-I-mediated TRIM. Material and methods. To this aim we compared the up-regulation of circulating sCD8 in plasma from patients suffering from the same pathology, but chronically transfused with two different blood derivatives: pre- and post-storage leucodepleted red blood cells which contain low and high levels of contaminating sHLA-I, respectively. Results. Significantly larger amounts of sCD8 circulating molecules were detectable in the plasma of patients transfused with post-storage leucodepleted red blood cells whose supernatants contained significantly larger amounts of sHLA-I contaminating molecules. Conclusion. With the limitation of indirect evidence, this report introduces a new facet of the bioactivity of sCD8 as a possible modulator of sHLA-I-mediated TRIM.
KW - SCD8.
KW - SHLA-I
KW - TRIM
UR - http://www.scopus.com/inward/record.url?scp=84893971494&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84893971494&partnerID=8YFLogxK
U2 - 10.2450/2012.0199-12
DO - 10.2450/2012.0199-12
M3 - Article
C2 - 23356971
AN - SCOPUS:84893971494
VL - 12
JO - Blood Transfusion
JF - Blood Transfusion
SN - 1723-2007
IS - SUPPL.1
ER -