Blue laser light inhibits biofilm formation in vitro and in vivo by inducing oxidative stress

Katia Rupel, Luisa Zupin, Giulia Ottaviani, Iris Bertani, Valentina Martinelli, Davide Porrelli, Simone Vodret, Roman Vuerich, Daniel Passos da Silva, Rossana Bussani, Sergio Crovella, Matthew Parsek, Vittorio Venturi, Roberto Di Lenarda, Matteo Biasotto, Serena Zacchigna

Research output: Contribution to journalArticlepeer-review


Resolution of bacterial infections is often hampered by both resistance to conventional antibiotic therapy and hiding of bacterial cells inside biofilms, warranting the development of innovative therapeutic strategies. Here, we report the efficacy of blue laser light in eradicating Pseudomonas aeruginosa cells, grown in planktonic state, agar plates and mature biofilms, both in vitro and in vivo, with minimal toxicity to mammalian cells and tissues. Results obtained using knock-out mutants point to oxidative stress as a relevant mechanism by which blue laser light exerts its anti-microbial effect. Finally, the therapeutic potential is confirmed in a mouse model of skin wound infection. Collectively, these data set blue laser phototherapy as an innovative approach to inhibit bacterial growth and biofilm formation, and thus as a realistic treatment option for superinfected wounds.

Original languageEnglish
Article number29
Journalnpj Biofilms and Microbiomes
Issue number1
Publication statusPublished - Dec 1 2019

ASJC Scopus subject areas

  • Biotechnology
  • Microbiology
  • Applied Microbiology and Biotechnology

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