BMAP-28 improves the efficacy of vancomycin in rat models of gram-positive cocci ureteral stent infection

Fiorenza Orlando, Roberto Ghiselli, Oscar Cirioni, Daniele Minardi, Linda Tomasinsig, Federico Mocchegiani, Carmela Silvestri, Barbara Skerlavaj, Alessandra Riva, Giovanni Muzzonigro, Vittorio Saba, Giorgio Scalise, Margherita Zanetti, Andrea Giacometti

Research output: Contribution to journalArticlepeer-review

Abstract

An experimental study was performed to evaluate the efficacy of BMAP-28 alone and in combination with vancomycin in animal models ureteral stent infection due to Enterococcus faecalis and Staphylococcus aureus. Study included a control group without bacterial challenge to evaluate the sterility of surgical procedure, a challenged control group that did not receive any antibiotic prophylaxis and for each bacterial strain three challenged groups that received (a) 10 mg/kg vancomycin intraperitoneally, immediately after stent implantation, (b) BMAP-28-coated ureteral stents where 0.2-cm2 sterile ureteral stents were incubated in 1 mg/l BMAP-28 solution for 30 min immediately before implantation and (c) intraperitoneal vancomycin plus BMAP-28-coated ureteral stent at the above concentrations. Experiments were performed in duplicate. Ureteral stents were explanted at day 5 following implantation and biofilm bacteria enumerated. Our data showed that rats that received intraperitoneal vancomycin showed the lowest bacterial numbers. BMAP-28 combined with vancomycin showed efficacies higher than that of each single compound. These results highlight the potential usefulness of this combination in preventing ureteral stent-associated in gram-positive infections.

Original languageEnglish
Pages (from-to)1118-1123
Number of pages6
JournalPeptides
Volume29
Issue number7
DOIs
Publication statusPublished - Jul 2008

Keywords

  • Animal model
  • Biofilm
  • Cathelicidins
  • Gram-positive cocci
  • Ureteral stent

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Physiology
  • Cellular and Molecular Neuroscience

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