BMX, a novel nonreceptor tyrosine kinase gene of the BTK/ITK/TEC/ TXK family located in chromosome Xp22.2

Luca Tamagnone, Isto Lahtinen, Tuija Mustonen, Kimmo Virtaneva, Fiona Francis, Francoise Muscatelli, Riitta Alitalo, C. I Edvard Smith, Catharina Larsson, Kari Alitalo

Research output: Contribution to journalArticlepeer-review

Abstract

The Bmx sequence was identified and cloned during our search for novel tyrosine kinase genes expressed in human bone marrow cells. Bmx cDNA comprises a long open reading frame of 675 amino acids, containing one SH3, one SH2 and one tyrosine kinase domain, which are about 70% identical with Btk, Itk and Tec and somewhat less with Txk tyrosine kinase sequences. The amino terminal sequences of these four tyrosine kinases are about 40% identical and each contains a so-called pleckstrin homology domain. The 2.7 kb Bmx mRNA was expressed in endothelial cells and several human tissues by Northern blotting and an 80 kD Bmx polypeptide was detected in human endothelial cells. Immunoprecipitates of COS cells transfected with a Bmx expression vector and NIH3T3 cells expressing a Bmx retrovirus contained a tyrosyl phosphorylated Bmx polypeptide of similar molecular weight. The BMX gene was located in chromosomal band Xp22.2 between the DXS197 and DXS207 loci. Interestingly, chromosome X also contains the closest relative of BMX, the BTK gene, implicated in X-linked agammaglobulinemia. The BMX gene thus encodes a novel nonreceptor tyrosine kinase, which may play a role in the growth and differentiation of hematopoietic cells.

Original languageEnglish
Pages (from-to)3683-3688
Number of pages6
JournalOncogene
Volume9
Issue number12
Publication statusPublished - Dec 1994

ASJC Scopus subject areas

  • Cancer Research
  • Genetics
  • Molecular Biology

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