Bombesin-like immunoreactivity in the avian gut and its localisation to a distinct cell type

C. M. Timson; J. M. Polak; J. Wharton; M. A. Ghatei; S. R. Bloom; L. Usellini; C. Capella; E. Solcia; M. R. Brown; A. G E Pearse

doi:10.1007/BF00496533

Bombesin-like immunoreactivity in the avian gut and its localisation to a distinct cell type

C. M. Timson, J. M. Polak, J. Wharton, M. A. Ghatei, S. R. Bloom, L. Usellini, C. Capella, E. Solcia, M. R. Brown, A. G E Pearse

IRCCS Fondazione Policlinico San Matteo

Research output: Contribution to journal › Article › peer-review

Abstract

The distribution of a bombesin-like immunoreactive peptide in the avian gastro-intestinal tract was analysed by combined radioimmunoassay and immunocytochemistry. Radioimmunoassay of tissue extracts showed that the largest quantities of bombesin-like immunoreactivity were present in the proventriculus (64.5±6.0 pmol/g) with smaller but still considerable amounts in the gizzard (40.0±6.0 pmol/g). Immunocytochemically the extractable bombesin-like immunoreactivity was localised in numerous endocrine cells. These, in the proventriculus, were found mainly in the deeper layers of the mucosa. Further study of these cells by the semi-thin/thin technique revealed the presence of characteristic secretory granules. The functional name BN is proposed for this cell type.

Original language	English
Pages (from-to)	213-221
Number of pages	9
Journal	Histochemistry
Volume	61
Issue number	2
DOIs	https://doi.org/10.1007/BF00496533
Publication status	Published - Jan 1979

ASJC Scopus subject areas

Anatomy
Medicine(all)

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10.1007/BF00496533

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@article{aa2c64c1327d4acea80705bc17a2a284,

title = "Bombesin-like immunoreactivity in the avian gut and its localisation to a distinct cell type",

abstract = "The distribution of a bombesin-like immunoreactive peptide in the avian gastro-intestinal tract was analysed by combined radioimmunoassay and immunocytochemistry. Radioimmunoassay of tissue extracts showed that the largest quantities of bombesin-like immunoreactivity were present in the proventriculus (64.5±6.0 pmol/g) with smaller but still considerable amounts in the gizzard (40.0±6.0 pmol/g). Immunocytochemically the extractable bombesin-like immunoreactivity was localised in numerous endocrine cells. These, in the proventriculus, were found mainly in the deeper layers of the mucosa. Further study of these cells by the semi-thin/thin technique revealed the presence of characteristic secretory granules. The functional name BN is proposed for this cell type.",

author = "Timson, {C. M.} and Polak, {J. M.} and J. Wharton and Ghatei, {M. A.} and Bloom, {S. R.} and L. Usellini and C. Capella and E. Solcia and Brown, {M. R.} and Pearse, {A. G E}",

year = "1979",

month = jan,

doi = "10.1007/BF00496533",

language = "English",

volume = "61",

pages = "213--221",

journal = "Zeitschrift f{\"u}r Zellforschung und Mikroskopische Anatomie. Abteilung Histochemie",

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publisher = "Springer Verlag",

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T1 - Bombesin-like immunoreactivity in the avian gut and its localisation to a distinct cell type

AU - Timson, C. M.

AU - Polak, J. M.

AU - Wharton, J.

AU - Ghatei, M. A.

AU - Bloom, S. R.

AU - Usellini, L.

AU - Capella, C.

AU - Solcia, E.

AU - Brown, M. R.

AU - Pearse, A. G E

PY - 1979/1

Y1 - 1979/1

N2 - The distribution of a bombesin-like immunoreactive peptide in the avian gastro-intestinal tract was analysed by combined radioimmunoassay and immunocytochemistry. Radioimmunoassay of tissue extracts showed that the largest quantities of bombesin-like immunoreactivity were present in the proventriculus (64.5±6.0 pmol/g) with smaller but still considerable amounts in the gizzard (40.0±6.0 pmol/g). Immunocytochemically the extractable bombesin-like immunoreactivity was localised in numerous endocrine cells. These, in the proventriculus, were found mainly in the deeper layers of the mucosa. Further study of these cells by the semi-thin/thin technique revealed the presence of characteristic secretory granules. The functional name BN is proposed for this cell type.

AB - The distribution of a bombesin-like immunoreactive peptide in the avian gastro-intestinal tract was analysed by combined radioimmunoassay and immunocytochemistry. Radioimmunoassay of tissue extracts showed that the largest quantities of bombesin-like immunoreactivity were present in the proventriculus (64.5±6.0 pmol/g) with smaller but still considerable amounts in the gizzard (40.0±6.0 pmol/g). Immunocytochemically the extractable bombesin-like immunoreactivity was localised in numerous endocrine cells. These, in the proventriculus, were found mainly in the deeper layers of the mucosa. Further study of these cells by the semi-thin/thin technique revealed the presence of characteristic secretory granules. The functional name BN is proposed for this cell type.

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JO - Zeitschrift für Zellforschung und Mikroskopische Anatomie. Abteilung Histochemie

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SN - 0948-6143

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ER -

Bombesin-like immunoreactivity in the avian gut and its localisation to a distinct cell type

Abstract

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