Abstract
The capacity of isolated chondrocytes to join separate masses of cartilage matrix was investigated with composites implanted in subcutaneous pouches in nude mice. Slices of articular cartilage were harvested from lambs and were devitalized by cyclic freezing and thawing. The slices were then either co-cultured with viable allogeneic lamb chondrocytes (experimental) or cultured without such chondrocytes (control). Composites of three slices were constructed with use of fibrin glue and were implanted in nude mice for periods ranging from 7 to 42 days. Bonding of the experimental matrices with viable chondrocytes was achieved at 28 and 42 days, as assessed by direct examination, histology, thymidine uptake, and fluorescence. No bonding occurred in the control composites without viable chondrocytes. We conclude that devitalized cartilage matrix is a scaffold to which isolated chondrocytes can attach and begin to repopulate.
| Original language | English |
|---|---|
| Pages (from-to) | 89-95 |
| Number of pages | 7 |
| Journal | Journal of Orthopaedic Research |
| Volume | 16 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Jan 1998 |
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ASJC Scopus subject areas
- Orthopedics and Sports Medicine
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Bonding of cartilage matrices with cultured chondrocytes : An experimental model. / Peretti, G. M.; Randolph, M. A.; Caruso, E. M.; Rossetti, F.; Zaleske, D. J.
In: Journal of Orthopaedic Research, Vol. 16, No. 1, 01.1998, p. 89-95.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Bonding of cartilage matrices with cultured chondrocytes
T2 - An experimental model
AU - Peretti, G. M.
AU - Randolph, M. A.
AU - Caruso, E. M.
AU - Rossetti, F.
AU - Zaleske, D. J.
PY - 1998/1
Y1 - 1998/1
N2 - The capacity of isolated chondrocytes to join separate masses of cartilage matrix was investigated with composites implanted in subcutaneous pouches in nude mice. Slices of articular cartilage were harvested from lambs and were devitalized by cyclic freezing and thawing. The slices were then either co-cultured with viable allogeneic lamb chondrocytes (experimental) or cultured without such chondrocytes (control). Composites of three slices were constructed with use of fibrin glue and were implanted in nude mice for periods ranging from 7 to 42 days. Bonding of the experimental matrices with viable chondrocytes was achieved at 28 and 42 days, as assessed by direct examination, histology, thymidine uptake, and fluorescence. No bonding occurred in the control composites without viable chondrocytes. We conclude that devitalized cartilage matrix is a scaffold to which isolated chondrocytes can attach and begin to repopulate.
AB - The capacity of isolated chondrocytes to join separate masses of cartilage matrix was investigated with composites implanted in subcutaneous pouches in nude mice. Slices of articular cartilage were harvested from lambs and were devitalized by cyclic freezing and thawing. The slices were then either co-cultured with viable allogeneic lamb chondrocytes (experimental) or cultured without such chondrocytes (control). Composites of three slices were constructed with use of fibrin glue and were implanted in nude mice for periods ranging from 7 to 42 days. Bonding of the experimental matrices with viable chondrocytes was achieved at 28 and 42 days, as assessed by direct examination, histology, thymidine uptake, and fluorescence. No bonding occurred in the control composites without viable chondrocytes. We conclude that devitalized cartilage matrix is a scaffold to which isolated chondrocytes can attach and begin to repopulate.
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UR - http://www.scopus.com/inward/citedby.url?scp=0031969903&partnerID=8YFLogxK
U2 - 10.1002/jor.1100160115
DO - 10.1002/jor.1100160115
M3 - Article
C2 - 9565078
AN - SCOPUS:0031969903
VL - 16
SP - 89
EP - 95
JO - Journal of Orthopaedic Research
JF - Journal of Orthopaedic Research
SN - 0736-0266
IS - 1
ER -