Bone cement extracts modulate the osteoprotegerin/osteoprotegerin-ligand expression in MG63 osteoblast-like cells

Donatella Granchi, Elisabetta Cenni, Lucia Savarino, Gabriela Ciapetti, Giulia Forbicini, Melania Vancini, Cecilia Maini, Nicola Baldini, Armando Giunti

Research output: Contribution to journalArticle

Abstract

The osteoprotegerin-ligand (OPG-L) has been identified as the essential factor required for osteoclastogenesis, and its effects are prevented by the osteoprotegerin (OPG). The OPG-L/OPG balance plays a crucial role in coordinating the sequence of osteoclast and osteoblast differentiation during the bone remodeling. The aim of the study was to investigate if polymethylmethacrylate-based cements are able to modulate the expression of OPG-L/OPG in MG63 cells, which are known to have high levels of OPG and inducible expression of OPG-L. Four radio-opaque cements, namely Sulfix-60®, CMW1®, CMW2® and CMW3®, were polymerized for either 1h or 7d. MG63 were incubated for 24h with culture medium only, cement extracts and 2μg/ml of human recombinant IL-1β as positive control. An RT-PCR was performed to detect the OPG and OPG-L expression, and the house-keeping gene, GAPDH, was used as a reference for the semi-quantitative analysis. An increase in the OPG-L band density was observed for all cements, and consequently, the OPG-L/OPG ratio also increased. The ability of bone cements to induce the expression of OPG-L could be a co-factor in the development of osteolysis at the bone-cement interface.

Original languageEnglish
Pages (from-to)2359-2365
Number of pages7
JournalBiomaterials
Volume23
Issue number11
DOIs
Publication statusPublished - 2002

Keywords

  • Bone cements
  • Hip prosthesis
  • Osteolysis
  • Osteoprotegerin
  • Osteoprotegerin-ligand

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering

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