Bone marrow concentrate and expanded mesenchymal stromal cell surnatants as cell-free approaches for the treatment of osteochondral defects in a preclinical animal model

Francesca Veronesi; Giovanna Desando; Milena Fini; Annapaola Parrilli; Roberta Lolli; Melania Maglio; Lucia Martini; Gianluca Giavaresi; Isabella Bartolotti; Brunella Grigolo; Maria Sartori

doi:10.1007/s00264-018-4202-6

Bone marrow concentrate and expanded mesenchymal stromal cell surnatants as cell-free approaches for the treatment of osteochondral defects in a preclinical animal model

Francesca Veronesi, Giovanna Desando, Milena Fini, Annapaola Parrilli, Roberta Lolli, Melania Maglio, Lucia Martini, Gianluca Giavaresi, Isabella Bartolotti, Brunella Grigolo, Maria Sartori

Istituti Ortopedici Rizzoli

Research output: Contribution to journal › Article › peer-review

Abstract

PURPOSE: To evaluate the regenerative potential of surnatants (SNs) from bone marrow concentrate (SN-BMC) and expanded mesenchymal stromal cells (SN-MSCs) loaded onto a collagen scaffold (SC) in comparison with cell-based treatments (BMC and MSCs) in an osteochondral (OC) defect model in rabbits.

METHODS: OC defects (3 × 5 mm) were created in the rabbit femoral condyles and treated with SC alone or combined with SN-BMC, SN-MSCs, BMC, and MSCs. In control groups, the defects were left untreated. At three and six months, the quality of regenerated tissue was evaluated with macroscopic, histologic, microtomographic, and immunohistochemical assessments. The production of several immunoenzymatic markers was measured in the synovial fluid.

RESULTS: All proposed treatments improved OC regeneration in comparison with untreated and SC-treated defects. Both BMC and MSCs showed a similar healing potential than their respective SNs, with the best performance exerted by BMC as demonstrated with macroscopic and histological scores and type I and II collagen results.

CONCLUSIONS: SNs loaded onto SC exerted a positive effect on OC defect regeneration, underlying the biological significance of the trophic factors, thus potentially opening new opportunities for the use of cell-free-based therapies. BMC was confirmed to be the most beneficial treatment.

Original language	English
Pages (from-to)	25-34
Number of pages	10
Journal	International Orthopaedics
Volume	43
Issue number	1
DOIs	https://doi.org/10.1007/s00264-018-4202-6
Publication status	Published - Jan 2019

Keywords

Bone marrow concentrate
Cell-free approach
In vivo model
Mesenchymal stromal cells
Osteochondral defect

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10.1007/s00264-018-4202-6

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Veronesi, F., Desando, G., Fini, M., Parrilli, A., Lolli, R., Maglio, M., Martini, L., Giavaresi, G., Bartolotti, I., Grigolo, B., & Sartori, M. (2019). Bone marrow concentrate and expanded mesenchymal stromal cell surnatants as cell-free approaches for the treatment of osteochondral defects in a preclinical animal model. International Orthopaedics, 43(1), 25-34. https://doi.org/10.1007/s00264-018-4202-6

Bone marrow concentrate and expanded mesenchymal stromal cell surnatants as cell-free approaches for the treatment of osteochondral defects in a preclinical animal model. / Veronesi, Francesca ; Desando, Giovanna ; Fini, Milena; Parrilli, Annapaola; Lolli, Roberta; Maglio, Melania ; Martini, Lucia ; Giavaresi, Gianluca; Bartolotti, Isabella; Grigolo, Brunella ; Sartori, Maria.

In: International Orthopaedics, Vol. 43, No. 1, 01.2019, p. 25-34.

Research output: Contribution to journal › Article › peer-review

Veronesi, F , Desando, G , Fini, M, Parrilli, A, Lolli, R, Maglio, M , Martini, L , Giavaresi, G, Bartolotti, I, Grigolo, B & Sartori, M 2019, 'Bone marrow concentrate and expanded mesenchymal stromal cell surnatants as cell-free approaches for the treatment of osteochondral defects in a preclinical animal model', International Orthopaedics, vol. 43, no. 1, pp. 25-34. https://doi.org/10.1007/s00264-018-4202-6

Veronesi, Francesca ; Desando, Giovanna ; Fini, Milena ; Parrilli, Annapaola ; Lolli, Roberta ; Maglio, Melania ; Martini, Lucia ; Giavaresi, Gianluca ; Bartolotti, Isabella ; Grigolo, Brunella ; Sartori, Maria. / Bone marrow concentrate and expanded mesenchymal stromal cell surnatants as cell-free approaches for the treatment of osteochondral defects in a preclinical animal model. In: International Orthopaedics. 2019 ; Vol. 43, No. 1. pp. 25-34.

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title = "Bone marrow concentrate and expanded mesenchymal stromal cell surnatants as cell-free approaches for the treatment of osteochondral defects in a preclinical animal model",

abstract = "PURPOSE: To evaluate the regenerative potential of surnatants (SNs) from bone marrow concentrate (SN-BMC) and expanded mesenchymal stromal cells (SN-MSCs) loaded onto a collagen scaffold (SC) in comparison with cell-based treatments (BMC and MSCs) in an osteochondral (OC) defect model in rabbits.METHODS: OC defects (3 × 5 mm) were created in the rabbit femoral condyles and treated with SC alone or combined with SN-BMC, SN-MSCs, BMC, and MSCs. In control groups, the defects were left untreated. At three and six months, the quality of regenerated tissue was evaluated with macroscopic, histologic, microtomographic, and immunohistochemical assessments. The production of several immunoenzymatic markers was measured in the synovial fluid.RESULTS: All proposed treatments improved OC regeneration in comparison with untreated and SC-treated defects. Both BMC and MSCs showed a similar healing potential than their respective SNs, with the best performance exerted by BMC as demonstrated with macroscopic and histological scores and type I and II collagen results.CONCLUSIONS: SNs loaded onto SC exerted a positive effect on OC defect regeneration, underlying the biological significance of the trophic factors, thus potentially opening new opportunities for the use of cell-free-based therapies. BMC was confirmed to be the most beneficial treatment.",

keywords = "Bone marrow concentrate, Cell-free approach, In vivo model, Mesenchymal stromal cells, Osteochondral defect",

author = "Francesca Veronesi and Giovanna Desando and Milena Fini and Annapaola Parrilli and Roberta Lolli and Melania Maglio and Lucia Martini and Gianluca Giavaresi and Isabella Bartolotti and Brunella Grigolo and Maria Sartori",

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doi = "10.1007/s00264-018-4202-6",

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T1 - Bone marrow concentrate and expanded mesenchymal stromal cell surnatants as cell-free approaches for the treatment of osteochondral defects in a preclinical animal model

AU - Veronesi, Francesca

AU - Desando, Giovanna

AU - Fini, Milena

AU - Parrilli, Annapaola

AU - Lolli, Roberta

AU - Maglio, Melania

AU - Martini, Lucia

AU - Giavaresi, Gianluca

AU - Bartolotti, Isabella

AU - Grigolo, Brunella

AU - Sartori, Maria

PY - 2019/1

Y1 - 2019/1

N2 - PURPOSE: To evaluate the regenerative potential of surnatants (SNs) from bone marrow concentrate (SN-BMC) and expanded mesenchymal stromal cells (SN-MSCs) loaded onto a collagen scaffold (SC) in comparison with cell-based treatments (BMC and MSCs) in an osteochondral (OC) defect model in rabbits.METHODS: OC defects (3 × 5 mm) were created in the rabbit femoral condyles and treated with SC alone or combined with SN-BMC, SN-MSCs, BMC, and MSCs. In control groups, the defects were left untreated. At three and six months, the quality of regenerated tissue was evaluated with macroscopic, histologic, microtomographic, and immunohistochemical assessments. The production of several immunoenzymatic markers was measured in the synovial fluid.RESULTS: All proposed treatments improved OC regeneration in comparison with untreated and SC-treated defects. Both BMC and MSCs showed a similar healing potential than their respective SNs, with the best performance exerted by BMC as demonstrated with macroscopic and histological scores and type I and II collagen results.CONCLUSIONS: SNs loaded onto SC exerted a positive effect on OC defect regeneration, underlying the biological significance of the trophic factors, thus potentially opening new opportunities for the use of cell-free-based therapies. BMC was confirmed to be the most beneficial treatment.

AB - PURPOSE: To evaluate the regenerative potential of surnatants (SNs) from bone marrow concentrate (SN-BMC) and expanded mesenchymal stromal cells (SN-MSCs) loaded onto a collagen scaffold (SC) in comparison with cell-based treatments (BMC and MSCs) in an osteochondral (OC) defect model in rabbits.METHODS: OC defects (3 × 5 mm) were created in the rabbit femoral condyles and treated with SC alone or combined with SN-BMC, SN-MSCs, BMC, and MSCs. In control groups, the defects were left untreated. At three and six months, the quality of regenerated tissue was evaluated with macroscopic, histologic, microtomographic, and immunohistochemical assessments. The production of several immunoenzymatic markers was measured in the synovial fluid.RESULTS: All proposed treatments improved OC regeneration in comparison with untreated and SC-treated defects. Both BMC and MSCs showed a similar healing potential than their respective SNs, with the best performance exerted by BMC as demonstrated with macroscopic and histological scores and type I and II collagen results.CONCLUSIONS: SNs loaded onto SC exerted a positive effect on OC defect regeneration, underlying the biological significance of the trophic factors, thus potentially opening new opportunities for the use of cell-free-based therapies. BMC was confirmed to be the most beneficial treatment.

KW - Bone marrow concentrate

KW - Cell-free approach

KW - In vivo model

KW - Mesenchymal stromal cells

KW - Osteochondral defect

U2 - 10.1007/s00264-018-4202-6

DO - 10.1007/s00264-018-4202-6

M3 - Article

C2 - 30324310

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JO - International Orthopaedics

JF - International Orthopaedics

SN - 0341-2695

IS - 1

ER -

Bone marrow concentrate and expanded mesenchymal stromal cell surnatants as cell-free approaches for the treatment of osteochondral defects in a preclinical animal model

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Keywords

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